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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8455R-3 |
| Symbol |
CG8455 |
Full Name |
CG8455 |
| CG No |
CG8455 |
Old CG No |
CG8455 |
| Synonyms |
CG8455 |
| Accession No (Link to NCBI) |
NM_164798.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTGGCGGACTTTGTGGTGCT |
| Primer Seq. 3' |
aatctagaggtaccCCTGGGTGCAAAACATGCAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTGGCGGAC TTTGTGGTGC TGCAAAAGTG CAAGTGGCCG GAGATAAAGC GAAAGAAGTA
0061 TGTGGACGAT CCGCTTAGGG CAATGATTCT AGCGGATCCN GCATCTGCTG GGACCGCATC
0121 GTGGTCACTG GCTGGACAAG CTCTACCGGG AGTGGCACAT GACGCGGGCC TTTCAGGCGG
0181 CATCACGTCT GTTCCAGCCC GACGTGGTCT TCGTCTTGGG CGATTTGTTC GNCGAGGGCG
0241 ACATGGTCAG CNACAAGCAG TTcCAGGAAT ATGTGTGGCG CTACCTGAAG ATGTTCCACC
0301 TACCACCGGG AATTNCGCTC ATCAGTGTCG CGGGAAATCA CGACGTGGGC TTCCATTACA
0361 AAATGCATCC CTTCTTTATG TCGCGTTTTG AGAGTTATCT GAACAACTCC TCGGTCAATT
0421 TGTACACCAT TAAACAAATC CATTTTGTGG TGATAAATTC GATGGCTATG GAAGGTGATG
0481 GTTGCATGTT TTGCACCCAG G |
| in silico PCR Fragment |
0001 TGTGGCGGAC TTTGTGGTGC TGCAAAAGTG CAAGTGGCCG GAGATAAAGC GAAAGAAGTA
0061 TGTGGACGAT CCGCTTAGGG CAATGATTCT AGCGGATCC- GCATCTGCTG GGACCGCATC
0121 GTGGTCACTG GCTGGACAAG CTCTACCGGG AGTGGCACAT GACGCGGGCC TTTCAGGCGG
0181 CATCACGTCT GTTCCAGCCC GACGTGGTCT TCGTCTTGGG CGATTTGTTC GACGAGGGCG
0241 ACATGGTCAG CGACAAGCAG TTCCAGGAAT ATGTGTGGCG CTACCTGAAG ATGTTCCACC
0301 TACCACCGGG AATTCCGCTC ATCAGTGTCG CGGGAAATCA TGACGTGGGC TTCCATTACA
0361 AAATGCATCC CTTCTTTATG TCGCGTTTTG AGAGTTATCT GAACAACTCC TCGGTCAATT
0421 TGTACACCAT TAAACAAATC CATTTTGTGG TGATAAATTC GATGGCTATG GAAGGTGATG
0481 GTTGCATGTT TTGCACCCAG G |
| Assemble Data |
8455R-3.IR_full 1 TGTGGCGGACTTTGTGGTGCTGCAAAAGTGCAAGTGGCCGGAGATAAAGCGAAAGAAGTA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8455R-3.in silico 1 TGTGGCGGACTTTGTGGTGCTGCAAAAGTGCAAGTGGCCGGAGATAAAGCGAAAGAAGTA 60
8455R-3.IR_full 61 TGTGGACGATCCGCTTAGGGCAATGATTCTAGCGGATCCNGCATCTGCTGGGACCGCATC 120
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
8455R-3.in silico 61 TGTGGACGATCCGCTTAGGGCAATGATTCTAGCGGATCC-GCATCTGCTGGGACCGCATC 120
8455R-3.IR_full 121 GTGGTCACTGGCTGGACAAGCTCTACCGGGAGTGGCACATGACGCGGGCCTTTCAGGCGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8455R-3.in silico 121 GTGGTCACTGGCTGGACAAGCTCTACCGGGAGTGGCACATGACGCGGGCCTTTCAGGCGG 180
8455R-3.IR_full 181 CATCACGTCTGTTCCAGCCCGACGTGGTCTTCGTCTTGGGCGATTTGTTCGNCGAGGGCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
8455R-3.in silico 181 CATCACGTCTGTTCCAGCCCGACGTGGTCTTCGTCTTGGGCGATTTGTTCGACGAGGGCG 240
8455R-3.IR_full 241 ACATGGTCAGCNACAAGCAGTTCCAGGAATATGTGTGGCGCTACCTGAAGATGTTCCACC 300
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
8455R-3.in silico 241 ACATGGTCAGCGACAAGCAGTTCCAGGAATATGTGTGGCGCTACCTGAAGATGTTCCACC 300
8455R-3.IR_full 301 TACCACCGGGAATTNCGCTCATCAGTGTCGCGGGAAATCACGACGTGGGCTTCCATTACA 360
|||||||||||||| ||||||||||||||||||||||||| |||||||||||||||||||
8455R-3.in silico 301 TACCACCGGGAATTCCGCTCATCAGTGTCGCGGGAAATCATGACGTGGGCTTCCATTACA 360
8455R-3.IR_full 361 AAATGCATCCCTTCTTTATGTCGCGTTTTGAGAGTTATCTGAACAACTCCTCGGTCAATT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8455R-3.in silico 361 AAATGCATCCCTTCTTTATGTCGCGTTTTGAGAGTTATCTGAACAACTCCTCGGTCAATT 420
8455R-3.IR_full 421 TGTACACCATTAAACAAATCCATTTTGTGGTGATAAATTCGATGGCTATGGAAGGTGATG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8455R-3.in silico 421 TGTACACCATTAAACAAATCCATTTTGTGGTGATAAATTCGATGGCTATGGAAGGTGATG 480
8455R-3.IR_full 481 GTTGCATGTTTTGCACCCAGG 501
|||||||||||||||||||||
8455R-3.in silico 481 GTTGCATGTTTTGCACCCAGG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135347.1 |
CG8455-RB, transcript variant B (CG8455), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_164798.1 |
CG8455-RA, transcript variant A (CG8455), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_142493.2 |
CG7717-RA, transcript variant A (Mekk1), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_169833.1 |
CG7717-RB, transcript variant B (Mekk1), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_142597.2 |
CG12254-RA (MED25), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_165465.1 |
CG1765-RB, transcript variant B (EcR), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164828.2 |
CG17834-RB, transcript variant B (CG17834), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_167636.1 |
CG32543-RA (CG32543), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132959.2 |
CG8949-RA (CG8949), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166064.1 |
CG30480-RA (CG30480), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140067.1 |
CG3654-RD (CG3654), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_001032080.1 |
CG33695-RA, transcript variant A (CG33695), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_001032076.1 |
CG33694-RA, transcript variant A (cana), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_080254.2 |
CG6392-RA (cmet), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_001014748.1 |
CG4805-RB, transcript variant B (ppk28), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_132941.2 |
CG4805-RA, transcript variant A (ppk28), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_168449.1 |
CG32076-RA (Alg10), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_176752.1 |
CG6835-RC, transcript variant C (GS), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167590.1 |
CG32495-RA, transcript variant A (CG32495), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_165595.1 |
CG30373-RA (CG30373), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079567.2 |
CG18802-RA (alpha-Man-II), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132719.2 |
CG32604-RA, transcript variant A (l(1)G0007), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001014485.1 |
CG33515-RA (CG33515), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001042853.1 |
CG12473-RA, transcript variant A (stnB), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001042854.1 |
CG12473-RB, transcript variant B (stnB), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_166161.1 |
CG30100-RA (CG30100), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080312.2 |
CG7803-RA, transcript variant A (z), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001038738.1 |
CG7803-RB, transcript variant B (z), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001042851.1 |
CG12500-RA, transcript variant A (stnA), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001042852.1 |
CG12500-RB, transcript variant B (stnA), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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