|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8444R-1 |
| Symbol |
CG8444 |
Full Name |
CG8444 |
| CG No |
CG8444 |
Old CG No |
CG8444 |
| Synonyms |
CG8444 |
| Accession No (Link to NCBI) |
NM_141619.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N.
An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity.
Proc Natl Acad Sci U S A (2020) 117(1) 464-471
[
PubMed ID = 31852821
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAACCGTCCCAAGGCAATCAG |
| Primer Seq. 3' |
aatctagaggtaccGCATCAGGACATTCGAGGGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAACCGTCCC AAGGCAATCA GTTTCAAGGG CAACGATGCA CTGGAAAGCC ACTACGTgGG
0061 TGATGTACTC TACGCTTCCA TGGGCAAcNC CGTNCAGCGG CGATNACCAA CTGGAACGGA
0121 CTGACCATCA ACGATCCCTT CAACCTGGCC AAGGGCGTCA TTCTGGTGCA TGTCCAGGGC
0181 ATTGGTCACG TGACCACCGC CGGCAACGTC AAGACCTACG AGCTGACAGG CTCCGGAACG
0241 GACGCCTCTC TGAACGCCCT GGCCGCCGAA CTGGAGGCAG CCAACGAGCC CGTCTGCGAT
0301 ATTAACTTTG AGCAGTTCGA CGATGGCGTT CAAGCCTGGA AGTCCTGCTT TGGTGATTTC
0361 GAGGCCCCAG CCGNCAAGCC CACCAAGCAC CTGAACCCCT CTCTGCACAC CGCTGACAAG
0421 CAGTTCCTCC AGGAGGTTGG ATTCATCAAC AGTGCCGCCG ATCATCTGGC TGAGATGGCC
0481 AAGCCCTCNG AATGTCCTGA TGC |
| in silico PCR Fragment |
0001 GAACCGTCCC AAGGCAATCA GTTTCAAGGG CAACGATGCA CTGGAAAGCC ACTACGTGGG
0061 TGATGTACTC TACGCTTCCA TGGGCAACGC CGT-CAGCGG CGAT-ACCAA CTGGAACGGA
0121 CTGACCATCA ACGATCCCTT CAACCTGGCC AAGGGCGTCA TTCTGGTGCA TGTCCAGGGC
0181 ATTGGTCACG TGACCACCGC CGGCAACGTC AAGACCTACG AGCTGACAGG CTCCGGAACG
0241 GACGCCTCTC TGAACGCCCT GGCCGCCGAA CTGGAGGCAG CCAACGAGCC CGTCTGCGAT
0301 ATTAACTTTG AGCAGTTCGA CGATGGCGTT CAAGCCTGGA AGTCCTGCTT TGGTGATTTC
0361 GAGGCCCCAG CCGCCAAGCC CACCAAGCAC CTGAACCCCT CTCTGCACAC CGCTGACAAG
0421 CAGTTCCTCC AGGAGGTTGG ATTCATCAAC AGTGCCGCCG ATCATCTGGC TGAGATGGCC
0481 AAGCCCTC-G AATGTCCTGA TGC |
| Assemble Data |
8444R-1.IR_full 1 AACCGTCCCAAGGCAATCAGTTTCAAGGGCAACGATGCACTGGAAAGCCACTACGTGGG 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 1 AACCGTCCCAAGGCAATCAGTTTCAAGGGCAACGATGCACTGGAAAGCCACTACGTGGG 59
8444R-1.IR_full 61 TGATGTACTCTACGCTTCCATGGGCAACNCCGTNCAGCGGCGATNACCAACTGGAACGGA 119
|||||||||||||||||||||||||||| |||| |||||||||| |||||||||||||||
8444R-1.in silico 61 TGATGTACTCTACGCTTCCATGGGCAACGCCGT-CAGCGGCGAT-ACCAACTGGAACGGA 119
8444R-1.IR_full 121 CTGACCATCAACGATCCCTTCAACCTGGCCAAGGGCGTCATTCTGGTGCATGTCCAGGGC 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 121 CTGACCATCAACGATCCCTTCAACCTGGCCAAGGGCGTCATTCTGGTGCATGTCCAGGGC 179
8444R-1.IR_full 181 ATTGGTCACGTGACCACCGCCGGCAACGTCAAGACCTACGAGCTGACAGGCTCCGGAACG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 181 ATTGGTCACGTGACCACCGCCGGCAACGTCAAGACCTACGAGCTGACAGGCTCCGGAACG 239
8444R-1.IR_full 241 GACGCCTCTCTGAACGCCCTGGCCGCCGAACTGGAGGCAGCCAACGAGCCCGTCTGCGAT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 241 GACGCCTCTCTGAACGCCCTGGCCGCCGAACTGGAGGCAGCCAACGAGCCCGTCTGCGAT 299
8444R-1.IR_full 301 ATTAACTTTGAGCAGTTCGACGATGGCGTTCAAGCCTGGAAGTCCTGCTTTGGTGATTTC 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 301 ATTAACTTTGAGCAGTTCGACGATGGCGTTCAAGCCTGGAAGTCCTGCTTTGGTGATTTC 359
8444R-1.IR_full 361 GAGGCCCCAGCCGNCAAGCCCACCAAGCACCTGAACCCCTCTCTGCACACCGCTGACAAG 419
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 361 GAGGCCCCAGCCGCCAAGCCCACCAAGCACCTGAACCCCTCTCTGCACACCGCTGACAAG 419
8444R-1.IR_full 421 CAGTTCCTCCAGGAGGTTGGATTCATCAACAGTGCCGCCGATCATCTGGCTGAGATGGCC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8444R-1.in silico 421 CAGTTCCTCCAGGAGGTTGGATTCATCAACAGTGCCGCCGATCATCTGGCTGAGATGGCC 479
8444R-1.IR_full 481 AAGCCCTCNGAATGTCCTGATGC 502
|||||||| ||||||||||||||
8444R-1.in silico 481 AAGCCCTC-GAATGTCCTGATGC 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
3 |
9 |
NM_141619.2 |
CG8444-RA (CG8444), mRNA |
| 0.41 |
2 |
7 |
2 |
3 |
NM_080212.1 |
CG5832-RA (Hmx), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_164653.1 |
CG31646-RA (CG31646), mRNA |
| 0 |
0 |
2 |
8 |
8 |
NM_078723.2 |
CG3022-RA, transcript variant A (GABA-B-R3), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_057619.3 |
CG3710-RA (TfIIS), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_143153.2 |
CG4719-RA (tankyrase), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_141508.1 |
CG7910-RA (CG7910), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_139719.2 |
CG10592-RA (CG10592), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_140196.2 |
CG7600-RA (CG7600), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_078653.1 |
CG18572-RA, transcript variant A (r), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_134495.2 |
CG12234-RA (Ranbp21), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_079507.2 |
CG2530-RA (corto), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206710.1 |
CG1372-RB, transcript variant B (yl), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_078596.2 |
CG1372-RA, transcript variant A (yl), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135896.3 |
CG4168-RA (CG4168), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_206765.1 |
CG18572-RB, transcript variant B (r), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169844.1 |
CG31149-RA (CG31149), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_134631.2 |
CG12446-RA (CG12446), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001042886.1 |
CG34109-RB (CG34109), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166571.1 |
CG30193-RA, transcript variant A (CG30193), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079204.1 |
CG7525-RA (Tie), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166575.1 |
CG30193-RF, transcript variant F (CG30193), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166574.1 |
CG30193-RE, transcript variant E (CG30193), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135123.2 |
CG9021-RA (CG9021), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166576.1 |
CG30193-RD, transcript variant D (CG30193), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166572.1 |
CG30193-RB, transcript variant B (CG30193), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166573.1 |
CG30193-RC, transcript variant C (CG30193), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136855.1 |
CG13193-RA (CG13193), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165525.1 |
CG11141-RA, transcript variant A (CG11141), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136438.2 |
CG11141-RB, transcript variant B (CG11141), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
|
