NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8380R-4

Symbol DAT Full Name Dopamine transporter

CG No CG8380 Old CG No CG8380

Synonyms CG8380, fmn, DAT

Accession No (Link to NCBI) NM_079039.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATCAAAGACGCCCACGCCAC

Primer Seq. 3'

aatctagaggtaccATGGATTCCCTCGCTGCGGTT

Predicted Fragment Size

IR fragment full Seq

0001 AATCAAAGAC GCCCACGCCA CACGATAACG ATAACAATAG CATCAGCGAC GAGCGCGAAA 
0061 CATGGAGCGG CAAAGTGGAT TTTTTATTAT CGGTTATTGG ATTCGCCGTC GATTTAGCAA 
0121 ACGTCTGGAG ATTTCCCTAT TTGTGCTACA AAAATGGCGG TGGCGCTTTT CTTGTGCCCT 
0181 ACGGCATTAT GTTGGTGGTC GGTGGCATTC CTCTGTTCTA TATGGAATTG GCCCTGGGTC 
0241 AGCACAATCG TAAGGGTGCC ATAACCTGCT GGGGTCGCTT GGTGCCCCTC TTCAAAGGAA 
0301 TTGGATATGC CGTGGTGCTG ATAGCCTTCT ATGTGGACTT CTATTACAAT GTGATTATTG 
0361 CCTGGTCGTT GCGTTTCTTT TTTGCATCCT TCACCAACTC GCTGCCTTGG ACGTCGTGTA 
0421 ATAATATTTG GAACACACCA AATTGTAGAC CGTTTGAGAG CCAAAATGCA TCTCGTGTTC 
0481 CGGTTATTGG TAACTATAGT GACTTGTATG CCATGGGAAA ATCAAAGCCT GCTCTACAAT 
0541 GAGACATATA TGAATGGTTC GAGCTTGGGA TACGTCAGCG GGTTGGCCAT GTGGAGGGTT 
0601 TTCAGTCCGC AGCATCGGAA TATTTTAACC GCTACATTTT TGGAGCTGAA CCGCAGCGAG 
0661 GGAATCCAT

in silico PCR Fragment

0001 AATCAAAGAC GCCCACGCCA CACGATAACG ATAACAATAG CATCAGCGAC GAGCGCGAAA 
0061 CATGGAGCGG CAAAGTGGAT TTTTTATTAT CGGTTATTGG ATTCGCCGTC GATTTAGCAA 
0121 ACGTCTGGAG ATTTCCCTAT TTGTGCTACA AAAATGGCGG TGGCGCTTTT CTTGTGCCCT 
0181 ACGGCATTAT GTTGGTGGTC GGTGGCATTC CTCTGTTCTA TATGGAATTG GCCCTGGGTC 
0241 AGCACAATCG TAAGGGTGCC ATAACCTGCT GGGGTCGCTT GGTGCCCCTC TTCAAAGGAA 
0301 TTGGATATGC CGTGGTGCTG ATAGCCTTCT ATGTGGACTT CTATTACAAT GTGATTATTG 
0361 CCTGGTCGTT GCGTTTCTTT TTTGCATCCT TCACCAACTC GCTGCCTTGG ACGTCGTGTA 
0421 ATAATATTTG GAACACACCA AATTGTAGAC CGTTTGAGAG CCAAAATGCA TCTCGTGTTC 
0481 CGGTTATTGG TAACTATAGT GACTTGTATG CCATGGG-AA ATCAAAGCCT GCTCTACAAT 
0541 GAGACATATA TGAATGGTTC GAGCTT-GGA TACGTCAGC- GGTTGGCCAT GTGGAGGGTT 
0601 TTCAGTCCGC AGCATCGGAA TATTTTAACC GCTACA-TTT TGGAGCTGAA CCGCAGCGAG 
0661 GGAATCCAT

Assemble Data


8380R-4.IR_full       1   AATCAAAGACGCCCACGCCACACGATAACGATAACAATAGCATCAGCGACGAGCGCGAAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     1   AATCAAAGACGCCCACGCCACACGATAACGATAACAATAGCATCAGCGACGAGCGCGAAA 60


8380R-4.IR_full       61  CATGGAGCGGCAAAGTGGATTTTTTATTATCGGTTATTGGATTCGCCGTCGATTTAGCAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     61  CATGGAGCGGCAAAGTGGATTTTTTATTATCGGTTATTGGATTCGCCGTCGATTTAGCAA 120


8380R-4.IR_full       121 ACGTCTGGAGATTTCCCTATTTGTGCTACAAAAATGGCGGTGGCGCTTTTCTTGTGCCCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     121 ACGTCTGGAGATTTCCCTATTTGTGCTACAAAAATGGCGGTGGCGCTTTTCTTGTGCCCT 180


8380R-4.IR_full       181 ACGGCATTATGTTGGTGGTCGGTGGCATTCCTCTGTTCTATATGGAATTGGCCCTGGGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     181 ACGGCATTATGTTGGTGGTCGGTGGCATTCCTCTGTTCTATATGGAATTGGCCCTGGGTC 240


8380R-4.IR_full       241 AGCACAATCGTAAGGGTGCCATAACCTGCTGGGGTCGCTTGGTGCCCCTCTTCAAAGGAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     241 AGCACAATCGTAAGGGTGCCATAACCTGCTGGGGTCGCTTGGTGCCCCTCTTCAAAGGAA 300


8380R-4.IR_full       301 TTGGATATGCCGTGGTGCTGATAGCCTTCTATGTGGACTTCTATTACAATGTGATTATTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     301 TTGGATATGCCGTGGTGCTGATAGCCTTCTATGTGGACTTCTATTACAATGTGATTATTG 360


8380R-4.IR_full       361 CCTGGTCGTTGCGTTTCTTTTTTGCATCCTTCACCAACTCGCTGCCTTGGACGTCGTGTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     361 CCTGGTCGTTGCGTTTCTTTTTTGCATCCTTCACCAACTCGCTGCCTTGGACGTCGTGTA 420


8380R-4.IR_full       421 ATAATATTTGGAACACACCAAATTGTAGACCGTTTGAGAGCCAAAATGCATCTCGTGTTC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8380R-4.in silico     421 ATAATATTTGGAACACACCAAATTGTAGACCGTTTGAGAGCCAAAATGCATCTCGTGTTC 480


8380R-4.IR_full       481 CGGTTATTGGTAACTATAGTGACTTGTATGCCATGGGAAAATCAAAGCCTGCTCTACAAT 540
                          ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8380R-4.in silico     481 CGGTTATTGGTAACTATAGTGACTTGTATGCCATGGG-AAATCAAAGCCTGCTCTACAAT 540


8380R-4.IR_full       541 GAGACATATATGAATGGTTCGAGCTTGGGATACGTCAGCGGGTTGGCCATGTGGAGGGTT 600
                          |||||||||||||||||||||||||| |||||||||||| ||||||||||||||||||||
8380R-4.in silico     541 GAGACATATATGAATGGTTCGAGCTT-GGATACGTCAGC-GGTTGGCCATGTGGAGGGTT 600


8380R-4.IR_full       601 TTCAGTCCGCAGCATCGGAATATTTTAACCGCTACATTTTTGGAGCTGAACCGCAGCGAG 660
                          |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
8380R-4.in silico     601 TTCAGTCCGCAGCATCGGAATATTTTAACCGCTACA-TTTTGGAGCTGAACCGCAGCGAG 660


8380R-4.IR_full       661 GGAATCCAT 669
                          |||||||||
8380R-4.in silico     661 GGAATCCAT 669

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	647	0	0	2	NM_079039.2	CG8380-RA (DAT), mRNA
1.54	10	7	13	12	NM_166779.2	CG1732-RB, transcript variant B (CG1732), mRNA
1.54	10	7	13	12	NM_143673.1	CG1732-RA, transcript variant A (CG1732), mRNA
0	0	3	3	3	NM_139658.2	CG11353-RB, transcript variant B (CG11353), mRNA
0	0	3	3	3	NM_168093.1	CG11353-RA, transcript variant A (CG11353), mRNA
0	0	2	3	6	NM_079984.2	CG7400-RA, transcript variant A (Fatp), mRNA
0	0	2	3	6	NM_164934.1	CG7400-RB, transcript variant B (Fatp), mRNA
0	0	2	3	4	NM_165871.1	CG8850-RB, transcript variant B (CG8850), mRNA
0	0	2	3	4	NM_136911.2	CG8850-RA, transcript variant A (CG8850), mRNA
0	0	2	3	2	NM_132120.1	CG14438-RA, transcript variant A (CG14438), mRNA
0	0	2	3	2	NM_167090.1	CG14438-RB, transcript variant B (CG14438), mRNA
0	0	2	2	2	NM_132701.2	CG11092-RA (CG11092), mRNA
0	0	1	4	4	NM_137841.2	CG4046-RA (RpS16), mRNA
0	0	1	3	7	NM_137449.2	CG5661-RA (Sema-5c), mRNA
0	0	1	3	4	NM_137806.2	CG5465-RA (MED16), mRNA
0	0	1	3	4	NM_142291.2	CG14896-RA (CG14896), mRNA
0	0	1	3	2	NM_079258.2	CG5939-RA, transcript variant A (Prm), mRNA
0	0	1	3	2	NM_168291.1	CG5939-RC, transcript variant C (Prm), mRNA
0	0	1	3	2	NM_168290.1	CG5939-RB, transcript variant B (Prm), mRNA
0	0	1	3	2	NM_168292.1	CG5939-RD, transcript variant D (Prm), mRNA
0	0	1	3	2	NM_165000.1	CG31759-RA, transcript variant A (CG31759), mRNA
0	0	1	3	2	NM_165001.1	CG31759-RB, transcript variant B (CG31759), mRNA
0	0	1	2	3	NM_143505.2	CG7920-RA, transcript variant A (CG7920), mRNA
0	0	0	5	11	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	5	9	NM_139970.2	CG6983-RA (CG6983), mRNA
0	0	0	5	6	NM_078843.4	CG3479-RB, transcript variant B (osp), mRNA
0	0	0	5	6	NM_165089.2	CG3479-RA, transcript variant A (osp), mRNA
0	0	0	5	4	NM_135088.2	CG6907-RA (CG6907), mRNA
0	0	0	5	3	NM_140070.2	CG3424-RA, transcript variant A (path), mRNA
0	0	0	5	3	NM_168349.1	CG3424-RC, transcript variant C (path), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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