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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8370R-2 
 Symbol CG8370  Full Name CG8370 
 CG No CG8370  Old CG No CG8370 
 Synonyms CG8370 
 Accession No (Link to NCBI) NM_137229.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCGTTCTCAGACGGTGACACT 
 Primer Seq. 3' 
aatctagaggtaccCGCTGTGATTGCCAGCTGTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCGTTCTCAG ACGGTGACAC TATTCAATCA GCACTCTAAT CGAACGCTCC AGCTGAACGC 
0061 GNGTGGCCGG ACCAAGTCCG GCGTTCTACA GCAGCTTCCT GGGCACGAGA GAGGTGCCGC 
0121 CGCAAGGCAA TACAACTTTC AACGTGGTGT TCCTACCGCG CCAACTAgGC GCCATTGCCG 
0181 CCGACCTTCT CATTCACACC TCCTTCGGCC AGGCCGAGTT GGCGGTACAG GGCGAGGGCA 
0241 GTGAGTGCCC GTACCGCCTA AAGCCGTTGG TGGGCATTAA GGCGCCCATG AATGCGACAC 
0301 TCACGCCAGA GATTCACATG TACANACCCG CACGAGCGTC CGCTGCAGAT ACTTGAGATA 
0361 TACAGCAGCG GCGGCGAGTT TCAGTTAGAG TTGCCCAGCG GTGGCTCGGA GGGCCCACAA 
0421 AATCTGTGGA AAATTCCTCC ACACACTTTA AAGCCGGTCA TTAGGATATC ATTTCACGGC 
0481 cgCACAGCTG GCAATCACAG CG 
 in silico PCR Fragment 
0001 GCGTTCTCAG ACGGTGACAC TATTCAATCA ACACTCCAAT CGAACGCTCC AGCTGAACGC 
0061 G-GTGGCCGG ACCAAGTCCG GCGTTCTACA GCAGCTTCCT GGGCACGAGA GAGGTGCCGC 
0121 CGCAAGGCAA TACAACTTTC AACGTGGTGT TCCTACCGCG CCAACTAGGC GCCATTGCCG 
0181 CCGACCTTCT CATTCACACC TCCTTCGGCC AGGCCGAGTT GGCGGTACAG GGCGAGGGCA 
0241 GTGAGTGCCC GTACCGCCTA AAGCCGTTGG TGGGCATTAA GGCGCCCATG AATGCGACAC 
0301 TCACGCCAGA GATTCACATG TACA-ACCCG CACGAGCGGC CGCTGCAGAT ACTTGAGATA 
0361 TACAGCAGCG GCGGCGAGTT TCAGTTAGAG TTGCCCAGCG GTGGCTCGGA GGGCCCACAA 
0421 AATCTGTGGA AAATTCCTCC ACACACTTTA AAGCCGGTCA TTAGGATATC ATTTCACGGC 
0481 CGCACAGCTG GCAATCACAG CG 
 Assemble Data 

8370R-2.IR_full       1   GCGTTCTCAGACGGTGACACTATTCAATCAGCACTCTAATCGAACGCTCCAGCTGAACGC 60
                          |||||||||||||||||||||||||||||| ||||| |||||||||||||||||||||||
8370R-2.in silico     1   GCGTTCTCAGACGGTGACACTATTCAATCAACACTCCAATCGAACGCTCCAGCTGAACGC 60


8370R-2.IR_full       61  GNGTGGCCGGACCAAGTCCGGCGTTCTACAGCAGCTTCCTGGGCACGAGAGAGGTGCCGC 120
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     61  G-GTGGCCGGACCAAGTCCGGCGTTCTACAGCAGCTTCCTGGGCACGAGAGAGGTGCCGC 120


8370R-2.IR_full       121 CGCAAGGCAATACAACTTTCAACGTGGTGTTCCTACCGCGCCAACTAGGCGCCATTGCCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     121 CGCAAGGCAATACAACTTTCAACGTGGTGTTCCTACCGCGCCAACTAGGCGCCATTGCCG 180


8370R-2.IR_full       181 CCGACCTTCTCATTCACACCTCCTTCGGCCAGGCCGAGTTGGCGGTACAGGGCGAGGGCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     181 CCGACCTTCTCATTCACACCTCCTTCGGCCAGGCCGAGTTGGCGGTACAGGGCGAGGGCA 240


8370R-2.IR_full       241 GTGAGTGCCCGTACCGCCTAAAGCCGTTGGTGGGCATTAAGGCGCCCATGAATGCGACAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     241 GTGAGTGCCCGTACCGCCTAAAGCCGTTGGTGGGCATTAAGGCGCCCATGAATGCGACAC 300


8370R-2.IR_full       301 TCACGCCAGAGATTCACATGTACANACCCGCACGAGCGTCCGCTGCAGATACTTGAGATA 360
                          |||||||||||||||||||||||| ||||||||||||| |||||||||||||||||||||
8370R-2.in silico     301 TCACGCCAGAGATTCACATGTACA-ACCCGCACGAGCGGCCGCTGCAGATACTTGAGATA 360


8370R-2.IR_full       361 TACAGCAGCGGCGGCGAGTTTCAGTTAGAGTTGCCCAGCGGTGGCTCGGAGGGCCCACAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     361 TACAGCAGCGGCGGCGAGTTTCAGTTAGAGTTGCCCAGCGGTGGCTCGGAGGGCCCACAA 420


8370R-2.IR_full       421 AATCTGTGGAAAATTCCTCCACACACTTTAAAGCCGGTCATTAGGATATCATTTCACGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     421 AATCTGTGGAAAATTCCTCCACACACTTTAAAGCCGGTCATTAGGATATCATTTCACGGC 480


8370R-2.IR_full       481 CGCACAGCTGGCAATCACAGCG 502
                          ||||||||||||||||||||||
8370R-2.in silico     481 CGCACAGCTGGCAATCACAGCG 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137229.2  CG8370-RA (CG8370), mRNA 
0   0  2  4  15  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  2  2  4  NM_137029.2  CG17034-RD, transcript variant D (CG17034), mRNA 
0   0  2  2  4  NM_165984.1  CG17034-RA, transcript variant A (CG17034), mRNA 
0   0  2  2  4  NM_170624.1  CG17034-RB, transcript variant B (CG17034), mRNA 
0   0  2  2  4  NM_170625.1  CG17034-RC, transcript variant C (CG17034), mRNA 
0   0  1  3  3  NM_057526.3  CG5920-RA (sop), mRNA 
0   0  1  2  3  NM_132206.1  CG2253-RA (Upf2), mRNA 
0   0  1  2  3  NM_142930.2  CG10198-RA (Nup98), mRNA 
0   0  1  2  2  NM_166223.1  CG6556-RA (cnk), mRNA 
0   0  1  2  2  NM_165204.1  CG31784-RB, transcript variant B (CG31784), mRNA 
0   0  1  2  2  NM_165203.1  CG31784-RA, transcript variant A (CG31784), mRNA 
0   0  0  5  5  NM_140212.1  CG7543-RA (CG7543), mRNA 
0   0  0  4  5  NM_170286.1  CG31079-RA (CG31079), mRNA 
0   0  0  4  4  NM_079155.2  CG9102-RA (bab2), mRNA 
0   0  0  4  4  NM_169657.1  CG5166-RB, transcript variant B (Atx2), mRNA 
0   0  0  4  4  NM_142209.2  CG5166-RA, transcript variant A (Atx2), mRNA 
0   0  0  4  4  NM_169658.2  CG5166-RC, transcript variant C (Atx2), mRNA 
0   0  0  4  4  NM_142361.2  CG5319-RA (CG5319), mRNA 
0   0  0  4  3  NM_001038850.1  CG9027-RC, transcript variant C (CG9027), mRNA 
0   0  0  4  3  NM_166152.1  CG8448-RA, transcript variant A (mrj), mRNA 
0   0  0  4  3  NM_168898.1  CG32436-RA (CG32436), mRNA 
0   0  0  4  3  NM_166154.1  CG8448-RC, transcript variant C (mrj), mRNA 
0   0  0  4  3  NM_166153.1  CG8448-RB, transcript variant B (mrj), mRNA 
0   0  0  4  3  NM_166155.1  CG8448-RD, transcript variant D (mrj), mRNA 
0   0  0  4  2  NM_057519.3  CG17998-RA (Gprk2), mRNA 
0   0  0  4  2  NM_135402.2  CG9296-RA (CG9296), mRNA 
0   0  0  3  8  NM_143656.1  CG1674-RB, transcript variant B (CG1674), mRNA 
0   0  0  3  7  NM_136983.2  CG17019-RA (CG17019), mRNA 
0   0  0  3  7  NM_176031.1  CG33119-RA (CG33119), mRNA 

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