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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8362R-2 |
| Symbol |
nmdyn-D7 |
Full Name |
nmdyn-D7 |
| CG No |
CG8362 |
Old CG No |
CG8362 |
| Synonyms |
CG8362, nmdyn-D7 |
| Accession No (Link to NCBI) |
NM_141669.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTATGTATCCGACAAGGCCGT |
| Primer Seq. 3' |
aatctagaggtaccTGGGCGACAGTTTCACACTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTATGTATCC GACAAGGCCG TGGAAGTGTT CGACCAGCGC AACAAGCGCA CATTTCTGCG
0061 ACGCACCAAG ATCCCGGAGC TGACCCAGCG CGACTTCTTT GTGGGCTCCA AGATCAATGT
0121 CTTTGGCCGG CAGTTTGACA TTGTGGATTA TGCTGACGAC ACTACGCGGA CAAACCTAGC
0181 CAAATATCGC AAGAAGGGAT TCGTTTTGCT CAAGAACAAT ATGTGGACGA AGCACCTGGG
0241 CAAGTTCCTA AAGACTCTGA TCGACAACAA GATTAACATA AACCAGGGCA TGATGGTTCA
0301 GTTCTCTCCC AAAATGGTAA CTCAGTTTCT GTCCGGCAAG GACACGACGG ATGTCTCCTC
0361 TTCAGTCCTT ATGAACGAGC TGCTGGCTGG TCCGGCCATT AGTCTGGAGC TAATCGGCGA
0421 CAACGTAGTG GAGACCATCA AGGCCTGCGC CCAGTACAAG AGCACAGAGG CGGNAAACAC
0481 CCAGTGTGAA ACTGTCGCCC A |
| in silico PCR Fragment |
0001 CTATGTATCC GACAAGGCCG TGGAAGTGTT CGACCAGCGC AACAAGCGCA CATTTCTGCG
0061 ACGCACCAAG ATCCCGGAGC TGACCCAGCG CGACTTCTTT GTGGGCTCCA AGATCAATGT
0121 CTTTGGCCGG CAGTTTGACA TTGTGGATTA TGCTGACGAC ACTACGCGGA CAAACCTAGC
0181 CAAATATCGC AAGAAGGGAT TCGTTTTGCT CAAGAACAAT ATGTGGACGA AGCACCTGGG
0241 CAAGTTCCTA AAGACTCTGA TCGACAACAA GATTAACATA AACCAGGGCA TGATGGTTCA
0301 GTTCTCTCCC AAAATGGTAA CTCAGTTTCT GTCCGGCAAG GACACGACGG ATGTCTCCTC
0361 TTCAGTCCTT ATGAACGAGC TGCTGGCTGG TCCGGCCATT AGTCTGGAGC TAATCGGCGA
0421 CAACGTAGTG GAGACCATCA AGGCCTGCGC CCAGTACAAG AGCACAGAGG CGG-AAACAC
0481 CCAGTGTGAA ACTGTCGCCC A |
| Assemble Data |
8362R-2.IR_full 1 CTATGTATCCGACAAGGCCGTGGAAGTGTTCGACCAGCGCAACAAGCGCACATTTCTGCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 1 CTATGTATCCGACAAGGCCGTGGAAGTGTTCGACCAGCGCAACAAGCGCACATTTCTGCG 60
8362R-2.IR_full 61 ACGCACCAAGATCCCGGAGCTGACCCAGCGCGACTTCTTTGTGGGCTCCAAGATCAATGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 61 ACGCACCAAGATCCCGGAGCTGACCCAGCGCGACTTCTTTGTGGGCTCCAAGATCAATGT 120
8362R-2.IR_full 121 CTTTGGCCGGCAGTTTGACATTGTGGATTATGCTGACGACACTACGCGGACAAACCTAGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 121 CTTTGGCCGGCAGTTTGACATTGTGGATTATGCTGACGACACTACGCGGACAAACCTAGC 180
8362R-2.IR_full 181 CAAATATCGCAAGAAGGGATTCGTTTTGCTCAAGAACAATATGTGGACGAAGCACCTGGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 181 CAAATATCGCAAGAAGGGATTCGTTTTGCTCAAGAACAATATGTGGACGAAGCACCTGGG 240
8362R-2.IR_full 241 CAAGTTCCTAAAGACTCTGATCGACAACAAGATTAACATAAACCAGGGCATGATGGTTCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 241 CAAGTTCCTAAAGACTCTGATCGACAACAAGATTAACATAAACCAGGGCATGATGGTTCA 300
8362R-2.IR_full 301 GTTCTCTCCCAAAATGGTAACTCAGTTTCTGTCCGGCAAGGACACGACGGATGTCTCCTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 301 GTTCTCTCCCAAAATGGTAACTCAGTTTCTGTCCGGCAAGGACACGACGGATGTCTCCTC 360
8362R-2.IR_full 361 TTCAGTCCTTATGAACGAGCTGCTGGCTGGTCCGGCCATTAGTCTGGAGCTAATCGGCGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8362R-2.in silico 361 TTCAGTCCTTATGAACGAGCTGCTGGCTGGTCCGGCCATTAGTCTGGAGCTAATCGGCGA 420
8362R-2.IR_full 421 CAACGTAGTGGAGACCATCAAGGCCTGCGCCCAGTACAAGAGCACAGAGGCGGNAAACAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
8362R-2.in silico 421 CAACGTAGTGGAGACCATCAAGGCCTGCGCCCAGTACAAGAGCACAGAGGCGG-AAACAC 480
8362R-2.IR_full 481 CCAGTGTGAAACTGTCGCCCA 501
|||||||||||||||||||||
8362R-2.in silico 481 CCAGTGTGAAACTGTCGCCCA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_141669.2 |
CG8362-RA (nmdyn-D7), mRNA |
| 0.62 |
3 |
2 |
3 |
5 |
NM_078564.2 |
CG1594-RA (hop), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_142072.2 |
CG9922-RA (CG9922), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_164457.1 |
CG3166-RA, transcript variant A (aop), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_078731.2 |
CG3166-RB, transcript variant B (aop), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_167687.1 |
CG11943-RB, transcript variant B (CG11943), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_134518.2 |
CG11943-RA, transcript variant A (CG11943), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_143379.2 |
CG1647-RA (CG1647), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_057915.3 |
CG9749-RA (Abi), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135018.1 |
CG2976-RA (CG2976), mRNA |
| 0 |
0 |
1 |
9 |
7 |
NM_136864.2 |
CG13185-RA (CG13185), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_135408.2 |
CG9466-RA (CG9466), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_057592.3 |
CG4265-RA (Uch), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_142090.1 |
CG9616-RA (CG9616), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143064.1 |
CG6369-RA (Smg6), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137975.1 |
CG30183-RA (CG30183), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_144347.2 |
CG12149-RA (c12.2), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_142590.1 |
CG4465-RA (CG4465), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206511.1 |
CG7675-RC, transcript variant C (CG7675), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_164519.1 |
CG3558-RB, transcript variant B (CG3558), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_143807.2 |
CG3558-RA, transcript variant A (CG3558), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_142460.2 |
CG7675-RB, transcript variant B (CG7675), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_169809.2 |
CG7675-RA, transcript variant A (CG7675), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_137086.2 |
CG8257-RA (CG8257), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142307.1 |
CG12783-RA (CG12783), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135124.1 |
CG14001-RA (bchs), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_139910.2 |
CG8209-RA (CG8209), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_138105.1 |
CG13594-RA (CG13594), mRNA |
| 0 |
0 |
0 |
3 |
13 |
NM_139424.1 |
CG11814-RA (CG11814), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_132252.3 |
CG1474-RA (Es2), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
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| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
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| |
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| VB |
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