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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8328R-2 
 Symbol HLHmdelta  Full Name E(spl) region transcript mdelta 
 CG No CG8328  Old CG No CG8328 
 Synonyms mdelta, E(spl)mdelta, mdelta0.5, E(Spl)-HLH-mdelta, E(spl)mC, mC, HLHMD, E(Spl) HLH-m delta, E(spl)-mdelta, E(spl)Mdelta, E(spl) mdelta, E(spl)delta, HLH-mdelta, HLHmdelta, HLHmC, CG8328 
 Accession No (Link to NCBI) NM_079779.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCCGTTCAGGGTCAGAGATTT 
 Primer Seq. 3' 
aatctagaggtaccTATCCTCTTCTCGCGGAGACT 
 Predicted Fragment Size 
484 
 IR fragment full Seq 
0001 gcCGNTNTCA GGGTCAGAGA TTTATGACCA AGACTCAGCA TTACCGCANG gtGACCAAAC 
0061 CCCTCCTGGA GCGCAAAAGA CGCGCCCGGA TGAACCTGTA TCTGGACGAA CTCAAGGATC 
0121 TCATCGTGGA CACCATGGAT GCGCAGGGcG AACAGGTCAG CAAGCTGGAG AAAGCTGACA 
0181 TCTTAGAGCT CACCGTCAAC TATCTGAAAG CGCAACAGCA GCAGCGAGNT GGCCAATCCT 
0241 CAATCCCCAC CACCCGACCA AGTTAACCTG GACAAATTTC GGGCCGGATA CACCCAGGCT 
0301 GCCTACGAGG TCTCGCACAT ATTCTCTACG GTTCCCGGCT TGGATCTCAA ATTCGGCACC 
0361 CACCTGATGA AGCAATTGGG TCACCAGCTC AAGGACATGA AACAGGAAGA AGAAATCATC 
0421 GATATGGCCG AAGAACCAGT TAATCTAGCC GACCAAAAAC GTTCAAAGTC TCCGCGAGAA 
0481 GAGGATA 
 in silico PCR Fragment 
0001 GCCG--TTCA GGGTCAGAGA TTTATGACCA AGACTCAGCA TTACCGCAAG GTGACCAAAC 
0061 CCCTCCTGGA GCGCAAAAGA CGCGCCCGGA TGAACCTGTA TCTGGACGAA CTCAAGGATC 
0121 TCATCGTGGA CACCATGGAT GCGCAGGGCG AACAGGTCAG CAAGCTGGAG AAAGCTGACA 
0181 TCTTAGAGCT CACCGTCAAC TATCTGAAAG CGCAACAGCA GCAGCGAG-T GGCCAATCCT 
0241 CAATCCCCAC CACCCGACCA AGTTAACCTG GACAAATTTC GGGCCGGATA CACCCAGGCT 
0301 GCCTACGAGG TCTCGCACAT ATTCTCTACG GTTCCCGGCT TGGATCTCAA ATTCGGCACC 
0361 CACCTGATGA AGCAATTGGG TCACCAGCTC AAGGACATGA AACAGGAAGA AGAAATCATC 
0421 GATATGGCCG AAGAACCAGT TAATCTAGCC GACCAAAAAC GTTCAAAGTC TCCGCGAGAA 
0481 GAGGATA 
 Assemble Data 

8328R-2.IR_full       1   GCCGNTNTCAGGGTCAGAGATTTATGACCAAGACTCAGCATTACCGCANGGTGACCAAAC 60
                          ||||   ||||||||||||||||||||||||||||||||||||||||| |||||||||||
8328R-2.in silico     1   GCCG--TTCAGGGTCAGAGATTTATGACCAAGACTCAGCATTACCGCAAGGTGACCAAAC 60


8328R-2.IR_full       61  CCCTCCTGGAGCGCAAAAGACGCGCCCGGATGAACCTGTATCTGGACGAACTCAAGGATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     61  CCCTCCTGGAGCGCAAAAGACGCGCCCGGATGAACCTGTATCTGGACGAACTCAAGGATC 120


8328R-2.IR_full       121 TCATCGTGGACACCATGGATGCGCAGGGCGAACAGGTCAGCAAGCTGGAGAAAGCTGACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     121 TCATCGTGGACACCATGGATGCGCAGGGCGAACAGGTCAGCAAGCTGGAGAAAGCTGACA 180


8328R-2.IR_full       181 TCTTAGAGCTCACCGTCAACTATCTGAAAGCGCAACAGCAGCAGCGAGNTGGCCAATCCT 240
                          |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
8328R-2.in silico     181 TCTTAGAGCTCACCGTCAACTATCTGAAAGCGCAACAGCAGCAGCGAG-TGGCCAATCCT 240


8328R-2.IR_full       241 CAATCCCCACCACCCGACCAAGTTAACCTGGACAAATTTCGGGCCGGATACACCCAGGCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     241 CAATCCCCACCACCCGACCAAGTTAACCTGGACAAATTTCGGGCCGGATACACCCAGGCT 300


8328R-2.IR_full       301 GCCTACGAGGTCTCGCACATATTCTCTACGGTTCCCGGCTTGGATCTCAAATTCGGCACC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     301 GCCTACGAGGTCTCGCACATATTCTCTACGGTTCCCGGCTTGGATCTCAAATTCGGCACC 360


8328R-2.IR_full       361 CACCTGATGAAGCAATTGGGTCACCAGCTCAAGGACATGAAACAGGAAGAAGAAATCATC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     361 CACCTGATGAAGCAATTGGGTCACCAGCTCAAGGACATGAAACAGGAAGAAGAAATCATC 420


8328R-2.IR_full       421 GATATGGCCGAAGAACCAGTTAATCTAGCCGACCAAAAACGTTCAAAGTCTCCGCGAGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8328R-2.in silico     421 GATATGGCCGAAGAACCAGTTAATCTAGCCGACCAAAAACGTTCAAAGTCTCCGCGAGAA 480


8328R-2.IR_full       481 GAGGATA 487
                          |||||||
8328R-2.in silico     481 GAGGATA 487



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   466  0  0  0  NM_079779.2  CG8328-RA (HLHmdelta), mRNA 
0   0  3  8  23  NM_079785.2  CG8346-RA (HLHm3), mRNA 
0   0  3  2  3  NM_166569.1  CG12781-RB, transcript variant B (nahoda), mRNA 
0   0  3  2  3  NM_079091.2  CG12781-RA, transcript variant A (nahoda), mRNA 
0   0  3  2  3  NM_057534.3  CG3242-RA (sob), mRNA 
0   0  2  4  13  NM_080505.2  CG8361-RA (HLHm7), mRNA 
0   0  2  4  8  NM_001043265.1  CG34139-RA (CG34139), mRNA 
0   0  1  7  11  NM_142975.2  CG5669-RA (CG5669), mRNA 
0   0  1  6  6  NM_137075.2  CG18568-RA (CG18568), mRNA 
0   0  1  4  11  NM_168892.1  CG18023-RB, transcript variant B (Eip78C), mRNA 
0   0  1  4  11  NM_079471.2  CG18023-RA, transcript variant A (Eip78C), mRNA 
0   0  1  4  8  NM_136982.2  CG3850-RA (sug), mRNA 
0   0  1  4  4  NM_166342.1  CG11961-RA, transcript variant A (CG11961), mRNA 
0   0  1  4  4  NM_137569.3  CG11961-RB, transcript variant B (CG11961), mRNA 
0   0  1  4  3  NM_133104.2  CG7282-RA (CG7282), mRNA 
0   0  1  4  2  NM_136147.2  CG10364-RA (msb1l), mRNA 
0   0  1  3  4  NM_001038769.1  CG14617-RC, transcript variant C (CG14617), mRNA 
0   0  1  3  2  NM_140952.1  CG5585-RA (CG5585), mRNA 
0   0  1  3  2  NM_078764.2  CG11607-RA (H2.0), mRNA 
0   0  1  2  16  NM_080364.3  CG5461-RA, transcript variant A (bun), mRNA 
0   0  1  2  16  NM_001042894.1  CG5461-RF, transcript variant F (bun), mRNA 
0   0  1  2  12  NM_132525.1  CG15740-RA (CG15740), mRNA 
0   0  1  2  8  NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   0  1  2  6  NM_206405.1  CG33286-RA (CG33286), mRNA 
0   0  1  2  5  NM_141644.2  CG9379-RA (by), mRNA 
0   0  1  2  4  NM_001014497.1  CG2225-RF, transcript variant F (CG2225), mRNA 
0   0  1  2  4  NM_165393.2  CG2225-RB, transcript variant B (CG2225), mRNA 
0   0  1  2  4  NM_165392.2  CG2225-RA, transcript variant A (CG2225), mRNA 
0   0  1  2  4  NM_135019.1  CG15627-RA (CG15627), mRNA 
0   0  1  2  4  NM_206020.2  CG2225-RE, transcript variant E (CG2225), mRNA 

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