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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8302R-3 |
| Symbol |
Cyp4aa1 |
Full Name |
Cyp4aa1 |
| CG No |
CG8302 |
Old CG No |
CG8302 |
| Synonyms |
4aa1, CG8302, Cyp4aa1 |
| Accession No (Link to NCBI) |
NM_137223.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGAGATCGGTTCTCCTCTCG |
| Primer Seq. 3' |
aatctagaggtaccTTCTTGATCGGCACACCCAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGAGATCGG TTCTCCTCTC GCTNAGACTC ACGGGTCCGC CATCGCTGCC ATTTCTGGGC
0061 AACTGCATGC TGGTCACGGA TAAGGACTTG ATGCGCAGAT GTGCCGGCAA gGCCNTCGAT
0121 CTATATGGAT CTCTGGTGCG GATCTGGGTG CTGCTCTTTC CCTTCTTTGC TGTCCTGGAG
0181 CCGGAGGATC TGCAGGTGAT ACTCTCCTCA AAGAAGCACA CCAACAAGGT CTTCTTCTAC
0241 CGACTGATGC ACAATTTCCT GGGTGATGGG CTGATAACCA GCAGTGGTTC CAAGTGGAGC
0301 AATCATCGAC GGCTCATCCA GCCAGCGTTT CATCACAATC TGCTGGAGAA GTTCATCGAC
0361 ACCTTTGTGG ATGCCTCGCA ATCGTTGTAC GAGAATCTGG ATGCCGAAGc CGTGGGAACG
0421 GAGATAAATA TTGCCAAGTA CGTGAACAAC TGTGTACTGG ACATACTAAA TGAGGCCGTT
0481 TTGGGTGTGC CGATCAAGAA |
| in silico PCR Fragment |
0001 TTGAGATCGG TTCTCCTCTC GCTGAGACTC ACGGGTCCGC CATCGCTGCC ATTTCTGGGC
0061 AACTGCATGC TGGTCACGGA TAAGGACTTG ATGCGCAGAT GTGCCGGCAA GGCCTTCGAT
0121 CTATATGGAT CTCTGGTGCG GATCTGGGTG CTGCTCTTTC CCTTCTTTGC TGTCCTGGAG
0181 CCGGAGGATC TGCAGGTGAT ACTCTCCTCG AAGAAGCACA CCAACAAGGT CTTCTTCTAC
0241 CGACTGATGC ACAATTTCCT GGGTGATGGG CTGATAACCA GCAGTGGTTC CAAGTGGAGC
0301 AATCATCGAC GGCTCATCCA GCCAGCGTTT CATCACAATC TGCTGGAGAA GTTCATCGAC
0361 ACCTTTGTGG ATGCCTCGCA ATCGTTGTAC GAGAATCTGG ATGCCGAAGC CGTGGGAACG
0421 GAGATAAATA TTGCCAAGTA CGTGAACAAC TGTGTACTGG ACATACTAAA TGAGGCCGTT
0481 TTGGGTGTGC CGATCAAGAA |
| Assemble Data |
8302R-3.IR_full 1 TTGAGATCGGTTCTCCTCTCGCTNAGACTCACGGGTCCGCCATCGCTGCCATTTCTGGGC 60
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
8302R-3.in silico 1 TTGAGATCGGTTCTCCTCTCGCTGAGACTCACGGGTCCGCCATCGCTGCCATTTCTGGGC 60
8302R-3.IR_full 61 AACTGCATGCTGGTCACGGATAAGGACTTGATGCGCAGATGTGCCGGCAAGGCCNTCGAT 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
8302R-3.in silico 61 AACTGCATGCTGGTCACGGATAAGGACTTGATGCGCAGATGTGCCGGCAAGGCCTTCGAT 120
8302R-3.IR_full 121 CTATATGGATCTCTGGTGCGGATCTGGGTGCTGCTCTTTCCCTTCTTTGCTGTCCTGGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8302R-3.in silico 121 CTATATGGATCTCTGGTGCGGATCTGGGTGCTGCTCTTTCCCTTCTTTGCTGTCCTGGAG 180
8302R-3.IR_full 181 CCGGAGGATCTGCAGGTGATACTCTCCTCAAAGAAGCACACCAACAAGGTCTTCTTCTAC 240
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
8302R-3.in silico 181 CCGGAGGATCTGCAGGTGATACTCTCCTCGAAGAAGCACACCAACAAGGTCTTCTTCTAC 240
8302R-3.IR_full 241 CGACTGATGCACAATTTCCTGGGTGATGGGCTGATAACCAGCAGTGGTTCCAAGTGGAGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8302R-3.in silico 241 CGACTGATGCACAATTTCCTGGGTGATGGGCTGATAACCAGCAGTGGTTCCAAGTGGAGC 300
8302R-3.IR_full 301 AATCATCGACGGCTCATCCAGCCAGCGTTTCATCACAATCTGCTGGAGAAGTTCATCGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8302R-3.in silico 301 AATCATCGACGGCTCATCCAGCCAGCGTTTCATCACAATCTGCTGGAGAAGTTCATCGAC 360
8302R-3.IR_full 361 ACCTTTGTGGATGCCTCGCAATCGTTGTACGAGAATCTGGATGCCGAAGCCGTGGGAACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8302R-3.in silico 361 ACCTTTGTGGATGCCTCGCAATCGTTGTACGAGAATCTGGATGCCGAAGCCGTGGGAACG 420
8302R-3.IR_full 421 GAGATAAATATTGCCAAGTACGTGAACAACTGTGTACTGGACATACTAAATGAGGCCGTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8302R-3.in silico 421 GAGATAAATATTGCCAAGTACGTGAACAACTGTGTACTGGACATACTAAATGAGGCCGTT 480
8302R-3.IR_full 481 TTGGGTGTGCCGATCAAGAA 500
||||||||||||||||||||
8302R-3.in silico 481 TTGGGTGTGCCGATCAAGAA 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137223.1 |
CG8302-RA (Cyp4aa1), mRNA |
| 0.2 |
1 |
2 |
3 |
4 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079866.2 |
CG1744-RA (chp), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140340.1 |
CG10971-RA, transcript variant A (CG10971), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_168513.1 |
CG10971-RB, transcript variant B (CG10971), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_139791.1 |
CG10144-RA (CG10144), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143059.1 |
CG13646-RA (CG13646), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142420.1 |
CG14315-RA (CG14315), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_135513.3 |
CG5722-RA (NPC1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169278.2 |
CG9356-RB, transcript variant B (CG9356), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141632.1 |
CG9356-RA, transcript variant A (CG9356), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135420.2 |
CG13101-RA, transcript variant A (CG13101), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_164841.1 |
CG13101-RB, transcript variant B (CG13101), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140043.3 |
CG4347-RA, transcript variant A (UGP), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168325.2 |
CG4347-RC, transcript variant C (UGP), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_140097.1 |
CG6749-RA (CG6749), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_078517.2 |
CG2175-RA, transcript variant A (dec-1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167133.1 |
CG2175-RC, transcript variant C (dec-1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167132.1 |
CG2175-RB, transcript variant B (dec-1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_136120.1 |
CG13085-RA (CG13085), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_079216.3 |
CG10541-RA (Tektin-C), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_143496.3 |
CG15523-RA (CG15523), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132345.1 |
CG12141-RA, transcript variant A (Aats-lys), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167196.1 |
CG12141-RB, transcript variant B (Aats-lys), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_170029.1 |
CG31161-RA (CG31161), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169249.1 |
CG31100-RA (CG31100), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142759.1 |
CG7084-RA, transcript variant A (CG7084), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169999.1 |
CG7084-RB, transcript variant B (CG7084), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_133049.1 |
CG5988-RA (upd2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079295.2 |
CG6539-RA (Dhh1), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
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