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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8293R-2 |
| Symbol |
Iap2 |
Full Name |
Inhibitor of apoptosis 2 |
| CG No |
CG8293 |
Old CG No |
CG8293 |
| Synonyms |
CG8293, DIAP2, DIAPII, diap2, Diap2, DIAP-2, DIAP, dIAP2, IAP, dIAP, DIHA, D-IAP2, dILP, D-iap2, Ilp, Diha, Diap, Iap2 |
| Accession No (Link to NCBI) |
NM_057779.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Kleino A, Valanne S, Ulvila J, Kallio J, Myllymäki H, Enwald H, Stöven S, Poidevin M, Ueda R, Hultmark D, Lemaitre B, Rämet M.
Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway.
EMBO J (2005) 24(19) 3423-34
[
PubMed ID = 16163390
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGCCCCTGAATGCCCCAGT |
| Primer Seq. 3' |
aatctagaggtaccTAAGGGGGCCCATTTGCACAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGGCCCCTG AATGCCCCAG TTTCCGCGGA GGATCTGGTC GCCAATGGTT TCTTTGCCAC
0061 GGGAAACTGG CTGGAGGCCG AGTGCCATTT CTGCCACGTG CGCATCGACC GCTGGGAATA
0121 CGGCGATCAA GTGGCGGAGC GCCANTCGAC GCTCCTCGCC CATCTGCTCC ATGGTTCTGG
0181 CTCCCAATCA CTGCGGCAAT GTTCCCAGGA GCCAGGAGAG CGACAACAAG GGAAACAGCG
0241 TAGTGGACAG CCCGGAGTCC TGCTCTTGTC CCGATCTCTT GTTGGAGGCC AATCGCTTGG
0301 TAACTTTCAA GGACTGGCCG AATCCCAACA TNCNCGcCGC AGGCTCTGGC AAAGGCAGGT
0361 TTCTACTACC TGAACCGTCT GGATCACGTG AAGTGTGTTT GGTGCAACGG AGTGATTGCC
0421 AAGTGGGAGA AGAACGACAA TGCCTTTGAA GAGCACAAGC GCTTTTTTCC CCAATGTCCT
0481 CGTGTGCAAA TGGGCCCCCT TA |
| in silico PCR Fragment |
0001 ATGGCCCCTG AATGCCCCAG TTTCCGCGGA GGATCTGGTC GCCAATGGTT TCTTTGCCAC
0061 GGGAAACTGG CTGGAGGCCG AGTGCCATTT CTGCCACGTG CGCATCGACC GCTGGGAATA
0121 CGGCGATCAA GTGGCGGAGC GCCA-TCGCC GCTCCTCGCC CATCTGCTCC ATGGTTCTGG
0181 CTCCCAATCA CTGCGGCAAT GTTCCCAGGA GCCAGGAGAG CGACAACGAG GGAAACAGCG
0241 TAGTGGACAG CCCGGAGTCC TGCTCTTGTC CCGATCTCTT GTTGGAGGCC AATCGCTTGG
0301 TAACTTTCAA GGACTGGCCG AATCCCAACA T-CACGCCGC AGGCTCTGGC AAAGGCAGGT
0361 TTCTACTACC TGAACCGTCT GGATCACGTG AAGTGTGTTT GGTGCAACGG AGTGATTGCC
0421 AAGTGGGAGA AGAACGACAA TGCCTTTGAA GAGCACAAGC GCTTTTTTCC CCAATGTCCT
0481 CGTGTGCAAA TGGGCCCCCT TA |
| Assemble Data |
8293R-2.IR_full 1 ATGGCCCCTGAATGCCCCAGTTTCCGCGGAGGATCTGGTCGCCAATGGTTTCTTTGCCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8293R-2.in silico 1 ATGGCCCCTGAATGCCCCAGTTTCCGCGGAGGATCTGGTCGCCAATGGTTTCTTTGCCAC 60
8293R-2.IR_full 61 GGGAAACTGGCTGGAGGCCGAGTGCCATTTCTGCCACGTGCGCATCGACCGCTGGGAATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8293R-2.in silico 61 GGGAAACTGGCTGGAGGCCGAGTGCCATTTCTGCCACGTGCGCATCGACCGCTGGGAATA 120
8293R-2.IR_full 121 CGGCGATCAAGTGGCGGAGCGCCANTCGACGCTCCTCGCCCATCTGCTCCATGGTTCTGG 180
|||||||||||||||||||||||| ||| |||||||||||||||||||||||||||||||
8293R-2.in silico 121 CGGCGATCAAGTGGCGGAGCGCCA-TCGCCGCTCCTCGCCCATCTGCTCCATGGTTCTGG 180
8293R-2.IR_full 181 CTCCCAATCACTGCGGCAATGTTCCCAGGAGCCAGGAGAGCGACAACAAGGGAAACAGCG 240
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
8293R-2.in silico 181 CTCCCAATCACTGCGGCAATGTTCCCAGGAGCCAGGAGAGCGACAACGAGGGAAACAGCG 240
8293R-2.IR_full 241 TAGTGGACAGCCCGGAGTCCTGCTCTTGTCCCGATCTCTTGTTGGAGGCCAATCGCTTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8293R-2.in silico 241 TAGTGGACAGCCCGGAGTCCTGCTCTTGTCCCGATCTCTTGTTGGAGGCCAATCGCTTGG 300
8293R-2.IR_full 301 TAACTTTCAAGGACTGGCCGAATCCCAACATNCNCGCCGCAGGCTCTGGCAAAGGCAGGT 360
||||||||||||||||||||||||||||||| | ||||||||||||||||||||||||||
8293R-2.in silico 301 TAACTTTCAAGGACTGGCCGAATCCCAACAT-CACGCCGCAGGCTCTGGCAAAGGCAGGT 360
8293R-2.IR_full 361 TTCTACTACCTGAACCGTCTGGATCACGTGAAGTGTGTTTGGTGCAACGGAGTGATTGCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8293R-2.in silico 361 TTCTACTACCTGAACCGTCTGGATCACGTGAAGTGTGTTTGGTGCAACGGAGTGATTGCC 420
8293R-2.IR_full 421 AAGTGGGAGAAGAACGACAATGCCTTTGAAGAGCACAAGCGCTTTTTTCCCCAATGTCCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8293R-2.in silico 421 AAGTGGGAGAAGAACGACAATGCCTTTGAAGAGCACAAGCGCTTTTTTCCCCAATGTCCT 480
8293R-2.IR_full 481 CGTGTGCAAATGGGCCCCCTTA 502
||||||||||||||||||||||
8293R-2.in silico 481 CGTGTGCAAATGGGCCCCCTTA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_176182.1 |
CG8293-RB, transcript variant B (Iap2), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_057779.3 |
CG8293-RA, transcript variant A (Iap2), mRNA |
| 0 |
0 |
8 |
3 |
2 |
NM_057575.2 |
CG8704-RA (dpn), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_164653.1 |
CG31646-RA (CG31646), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_136533.1 |
CG11641-RA (pdm3), mRNA |
| 0 |
0 |
2 |
7 |
2 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_080094.2 |
CG9696-RA, transcript variant A (dom), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_166446.1 |
CG9696-RD, transcript variant D (dom), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_176244.1 |
CG9696-RE, transcript variant E (dom), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_166270.1 |
CG5738-RB, transcript variant B (lolal), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_080039.2 |
CG5738-RA, transcript variant A (lolal), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_166271.1 |
CG5738-RC, transcript variant C (lolal), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_166272.1 |
CG5738-RD, transcript variant D (lolal), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_168777.1 |
CG6874-RB, transcript variant B (l(3)neo26), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_140804.2 |
CG6874-RA, transcript variant A (l(3)neo26), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142863.1 |
CG6726-RA, transcript variant A (CG6726), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_170048.1 |
CG6726-RB, transcript variant B (CG6726), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078562.2 |
CG1639-RA (l(1)10Bb), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_132792.2 |
CG6227-RA (CG6227), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_143344.2 |
CG5520-RA (Gp93), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NR_001327.1 |
CG5520-RA (Gp93), mRNA, mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078847.2 |
CG7595-RB, transcript variant B (ck), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057944.3 |
CG12249-RB, transcript variant B (mira), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165099.1 |
CG7595-RA, transcript variant A (ck), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057943.3 |
CG12249-RA, transcript variant A (mira), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_144348.2 |
CG12135-RA (c12.1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079446.2 |
CG8637-RA (trc), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_132249.1 |
CG1440-RA (CG1440), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_057618.2 |
CG3399-RA, transcript variant A (capu), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_164567.1 |
CG3399-RB, transcript variant B (capu), mRNA |
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