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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8210R-2 |
| Symbol |
Vha14 |
Full Name |
Vacuolar H[+] ATPase 14kD subunit |
| CG No |
CG8210 |
Old CG No |
CG8210 |
| Synonyms |
unnamed, V-ATPase, vha14, ATPase, CG8210, Vha14 |
| Accession No (Link to NCBI) |
NM_057621.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76
[
PubMed ID = 26551273
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTCTGCACTCGGCAATCAAG |
| Primer Seq. 3' |
aatctagaggtaccCACCAGATCCTCCGGATTGAA |
| Predicted Fragment Size |
366 |
| IR fragment full Seq |
0001 GNTCTGCACT CGGCAATCAA GGNAAAACTG ATCAGCGTTA TCGGCGACGA GGACACCTGT
0061 GTGGGCTTTC TGCTCGGCGG AGTGGGCGAG ATCAACAAGA ATCGCCATCC CAACTNTATG
0121 GTGGTCGACA AAAATACGGC CGTCAGCGAA CTGGAGGACT GTTTCAAGCG TTTCCTTAAG
0181 CGGGACGATA TCGACATCAT TCTAATCAAC CAGAACTNGC GCCGAGCTTA TTCGTCATGT
0241 GATCGATGCC CATACGTCGC CCGTGCCCGC TGTTTTGGAG ATTCCCTCCA AGGACCATCC
0301 GTACGACGCC AGCAAGGACT CCATTTCTGC GTCGCgNCCG CGGCATGTTC AATCCGGAGG
0361 ATCTGGTG |
| in silico PCR Fragment |
0001 GCTCTGCACT CGGCAATCAA GGGAAAACTG ATCAGCGTTA TCGGCGACGA GGACACCTGT
0061 GTGGGCTTTC TGCTCGGCGG AGTGGGCGAG ATCAACAAGA ATCGCCATCC CAACTTTATG
0121 GTGGTCGACA AAAATACGGC CGTCAGCGAA CTGGAGGACT GTTTCAAGCG TTTCCTTAAG
0181 CGGGACGATA TCGACATCAT TCTAATCAAC CAGAACT-GC GCCGAGCTTA TTCGTCATGT
0241 GATCGATGCC CATACGTCGC CCGTGCCCGC TGTTTTGGAG ATTCCCTCCA AGGACCATCC
0301 GTACGACGCC AGCAAGGACT CCA-TTCTGC GTCGCGCCCG CGGCATGTTC AATCCGGAGG
0361 ATCTGGTG |
| Assemble Data |
8210R-2.IR_full 1 GNTCTGCACTCGGCAATCAAGGNAAAACTGATCAGCGTTATCGGCGACGAGGACACCTGT 60
| |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
8210R-2.in silico 1 GCTCTGCACTCGGCAATCAAGGGAAAACTGATCAGCGTTATCGGCGACGAGGACACCTGT 60
8210R-2.IR_full 61 GTGGGCTTTCTGCTCGGCGGAGTGGGCGAGATCAACAAGAATCGCCATCCCAACTNTATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
8210R-2.in silico 61 GTGGGCTTTCTGCTCGGCGGAGTGGGCGAGATCAACAAGAATCGCCATCCCAACTTTATG 120
8210R-2.IR_full 121 GTGGTCGACAAAAATACGGCCGTCAGCGAACTGGAGGACTGTTTCAAGCGTTTCCTTAAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8210R-2.in silico 121 GTGGTCGACAAAAATACGGCCGTCAGCGAACTGGAGGACTGTTTCAAGCGTTTCCTTAAG 180
8210R-2.IR_full 181 CGGGACGATATCGACATCATTCTAATCAACCAGAACTNGCGCCGAGCTTATTCGTCATGT 240
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8210R-2.in silico 181 CGGGACGATATCGACATCATTCTAATCAACCAGAACT-GCGCCGAGCTTATTCGTCATGT 240
8210R-2.IR_full 241 GATCGATGCCCATACGTCGCCCGTGCCCGCTGTTTTGGAGATTCCCTCCAAGGACCATCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8210R-2.in silico 241 GATCGATGCCCATACGTCGCCCGTGCCCGCTGTTTTGGAGATTCCCTCCAAGGACCATCC 300
8210R-2.IR_full 301 GTACGACGCCAGCAAGGACTCCATTTCTGCGTCGCGNCCGCGGCATGTTCAATCCGGAGG 360
||||||||||||||||||||||| |||||||||||| |||||||||||||||||||||||
8210R-2.in silico 301 GTACGACGCCAGCAAGGACTCCA-TTCTGCGTCGCGCCCGCGGCATGTTCAATCCGGAGG 360
8210R-2.IR_full 361 ATCTGGTG 368
||||||||
8210R-2.in silico 361 ATCTGGTG 368
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
348 |
0 |
0 |
0 |
NM_057621.3 |
CG8210-RA (Vha14), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057576.3 |
CG6407-RA (Wnt5), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_167402.1 |
CG1517-RC, transcript variant C (na), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_167401.1 |
CG1517-RB, transcript variant B (na), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141676.2 |
CG12950-RA (CG12950), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164845.2 |
CG31708-RA, transcript variant A (CG31708), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164846.2 |
CG31708-RB, transcript variant B (CG31708), mRNA |
| 0 |
0 |
1 |
2 |
17 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168638.2 |
CG6117-RB, transcript variant B (Pka-C3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079373.2 |
CG6117-RA, transcript variant A (Pka-C3), mRNA |
| 0 |
0 |
0 |
11 |
18 |
NM_141357.1 |
CG1076-RA (CG1076), mRNA |
| 0 |
0 |
0 |
8 |
6 |
NM_141576.1 |
CG11775-RA (CG11775), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_058029.3 |
CG4882-RA (CG4882), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_142259.2 |
CG10278-RA (GATAe), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_001032076.1 |
CG33694-RA, transcript variant A (cana), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_001032080.1 |
CG33695-RA, transcript variant A (CG33695), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001043332.1 |
CG2005-RE, transcript variant E (Ptp99A), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164640.1 |
CG14021-RA, transcript variant A (CG14021), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135079.4 |
CG14021-RB, transcript variant B (CG14021), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079325.2 |
CG5433-RA (Klc), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141353.1 |
CG15580-RA, transcript variant A (CG15580), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169124.1 |
CG15580-RB, transcript variant B (CG15580), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_130606.2 |
CG3573-RA (CG3573), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_143362.2 |
CG14064-RA (beat-VI), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_078604.3 |
CG32592-RA (hiw), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_001032231.1 |
CG18003-RA, transcript variant A (CG18003), mRNA |
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