NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8190R-2

Symbol eIF2B-gamma Full Name eIF2B-gamma

CG No CG8190 Old CG No CG8190

Synonyms CG8190, eIF2Bgamma, eIF2B-gamma

Accession No (Link to NCBI) NM_137202.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGGTGGGTCCATATCCTCTG

Primer Seq. 3'

aatctagaggtaccAACCTTCCGCGGTTCTTGAGC

Predicted Fragment Size

IR fragment full Seq

0001 CCGgTGGGTC CATATCCTCT GAGTTTGGTA TCCCTTAAAT CTGCTGCAAC AACACAACTT 
0061 TACAGAGGTC ATTGTTGTGG TACTGGAGCA AGAAAAACTA GAGATCCAAT CTGCTCTGGA 
0121 AAACACTCCC TTAAAGCTGA GACTGGACTA TGCCACGATT CCTAGTGACG GGGATTTCGG 
0181 AACTGCCGAC AGTCTGCGCT ACATATACGA TAAGATCAAG TCGGACTTTC TGGTGGTCAG 
0241 CTGCGACCTG GTCAGCAATG TCAGCCTATA TCCGCTGATC AACAAGTTTC GGGAACATGA 
0301 TGCCGCCCTT GCAATGCTCC TATTTCCCAG CGGATTTGAG TCGGACGTTG TTATGCCCGG 
0361 TCCCAAAAGC AAGCACAAAC CGGAAAGGGA TTTGATTGGC ATCCATGCGG CCACTCAGAG 
0421 ATTGGCTTTC GTATCCGCCG CCAGTGACTG CGAGGAAACA CTCAACATCC AGCGACATCT 
0481 GCTCAAGAAC CGCGGAAGGT T

in silico PCR Fragment

0001 CCGGTGGGTC CATATCCTCT GA-TTTGGTA TCCCTTAAAT CTGCTGCAAC AACACAACTT 
0061 TACAGAGGTC ATTGTTGTGG TACTGGAGCA AGAAAAACTA GAGATCCAAT CTGCTCTGGA 
0121 AAACACTCCC TTAAAGCTGA GACTGGACTA TGCCACGATT CCTAGTGACG GGGATTTCGG 
0181 AACTGCCGAC AGTCTGCGCT ACATATACGA TAAGATCAAG TCGGACTTTC TGGTGGTCAG 
0241 CTGCGACCTG GTCAGCAATG TCAGCCTATA TCCGCTGATC AACAAGTTTC GGGAACATGA 
0301 TGCCGCCCTT GCAATGCTCC TATTTCCCAG CGGATTTGAG TCGGACGTTG TTATGCCCGG 
0361 TCCCAAAAGC AAGCACAAAC CGGAAAGGGA TTTGATTGGC ATCCATGCGG CCACTCAGAG 
0421 ATTGGCTTTC GTATCCGCCG CCAGTGACTG CGAGGAAACA CTCAACATCC AGCGACATCT 
0481 GCTCAAGAAC CGCGGAAGGT T

Assemble Data


8190R-2.IR_full       1   CCGGTGGGTCCATATCCTCTGAGTTTGGTATCCCTTAAATCTGCTGCAACAACACAACTT 60
                          |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
8190R-2.in silico     1   CCGGTGGGTCCATATCCTCTGA-TTTGGTATCCCTTAAATCTGCTGCAACAACACAACTT 60


8190R-2.IR_full       61  TACAGAGGTCATTGTTGTGGTACTGGAGCAAGAAAAACTAGAGATCCAATCTGCTCTGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     61  TACAGAGGTCATTGTTGTGGTACTGGAGCAAGAAAAACTAGAGATCCAATCTGCTCTGGA 120


8190R-2.IR_full       121 AAACACTCCCTTAAAGCTGAGACTGGACTATGCCACGATTCCTAGTGACGGGGATTTCGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     121 AAACACTCCCTTAAAGCTGAGACTGGACTATGCCACGATTCCTAGTGACGGGGATTTCGG 180


8190R-2.IR_full       181 AACTGCCGACAGTCTGCGCTACATATACGATAAGATCAAGTCGGACTTTCTGGTGGTCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     181 AACTGCCGACAGTCTGCGCTACATATACGATAAGATCAAGTCGGACTTTCTGGTGGTCAG 240


8190R-2.IR_full       241 CTGCGACCTGGTCAGCAATGTCAGCCTATATCCGCTGATCAACAAGTTTCGGGAACATGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     241 CTGCGACCTGGTCAGCAATGTCAGCCTATATCCGCTGATCAACAAGTTTCGGGAACATGA 300


8190R-2.IR_full       301 TGCCGCCCTTGCAATGCTCCTATTTCCCAGCGGATTTGAGTCGGACGTTGTTATGCCCGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     301 TGCCGCCCTTGCAATGCTCCTATTTCCCAGCGGATTTGAGTCGGACGTTGTTATGCCCGG 360


8190R-2.IR_full       361 TCCCAAAAGCAAGCACAAACCGGAAAGGGATTTGATTGGCATCCATGCGGCCACTCAGAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     361 TCCCAAAAGCAAGCACAAACCGGAAAGGGATTTGATTGGCATCCATGCGGCCACTCAGAG 420


8190R-2.IR_full       421 ATTGGCTTTCGTATCCGCCGCCAGTGACTGCGAGGAAACACTCAACATCCAGCGACATCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8190R-2.in silico     421 ATTGGCTTTCGTATCCGCCGCCAGTGACTGCGAGGAAACACTCAACATCCAGCGACATCT 480


8190R-2.IR_full       481 GCTCAAGAACCGCGGAAGGTT 501
                          |||||||||||||||||||||
8190R-2.in silico     481 GCTCAAGAACCGCGGAAGGTT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137202.2	CG8190-RA (eIF2B-gamma), mRNA
0	0	2	4	5	NM_170228.1	CG4548-RA, transcript variant A (XNP), mRNA
0	0	2	4	5	NM_143141.2	CG4548-RB, transcript variant B (XNP), mRNA
0	0	2	2	5	NM_057743.3	CG18290-RA, transcript variant A (Act87E), mRNA
0	0	1	6	3	NM_142974.2	CG17786-RA (CG17786), mRNA
0	0	1	3	3	NM_137690.2	CG10543-RA, transcript variant A (CG10543), mRNA
0	0	1	3	2	NM_166416.1	CG10543-RB, transcript variant B (CG10543), mRNA
0	0	1	3	2	NM_132774.1	CG9072-RA (CG9072), mRNA
0	0	1	3	2	NM_176241.1	CG10543-RC, transcript variant C (CG10543), mRNA
0	0	1	3	2	NM_170531.3	CG1447-RA, transcript variant A (Ptx1), mRNA
0	0	1	3	2	NM_206591.1	CG1447-RC, transcript variant C (Ptx1), mRNA
0	0	1	2	3	NM_079617.3	CG7855-RA (timeout), mRNA
0	0	0	7	29	NM_079919.2	CG5700-RB (prc), mRNA
0	0	0	6	2	NM_176734.1	CG33206-RA, transcript variant A (l(1)G0168), mRNA
0	0	0	4	7	NM_132084.2	CG12219-RA (CG12219), mRNA
0	0	0	4	6	NM_176712.1	CG33181-RA (CG33181), mRNA
0	0	0	4	5	NM_001043114.1	CG33484-RD, transcript variant D (zormin), mRNA
0	0	0	4	5	NM_001031967.1	CG7749-RB, transcript variant B (fat2), mRNA
0	0	0	4	5	NM_140914.2	CG7749-RA, transcript variant A (fat2), mRNA
0	0	0	4	5	NM_078611.2	CG8288-RA (mRpL3), mRNA
0	0	0	4	5	NM_164774.1	CG7115-RA, transcript variant A (CG7115), mRNA
0	0	0	4	4	NM_001043112.1	CG33484-RB, transcript variant B (zormin), mRNA
0	0	0	4	4	NM_206240.1	CG33484-RA, transcript variant A (zormin), mRNA
0	0	0	4	4	NM_001043113.1	CG33484-RC, transcript variant C (zormin), mRNA
0	0	0	4	3	NM_133132.1	CG14194-RA (CG14194), mRNA
0	0	0	4	3	NM_135547.1	CG5056-RA (CG5056), mRNA
0	0	0	4	3	NM_139775.1	CG13296-RA (CG13296), mRNA
0	0	0	4	3	NM_168290.1	CG5939-RB, transcript variant B (Prm), mRNA
0	0	0	4	3	NM_079258.2	CG5939-RA, transcript variant A (Prm), mRNA
0	0	0	4	2	NM_168291.1	CG5939-RC, transcript variant C (Prm), mRNA

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