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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8182R-1 |
| Symbol |
GalNAc-T1 |
Full Name |
GalNAc-T1 |
| CG No |
CG8182 |
Old CG No |
CG8182 |
| Synonyms |
pgant1, CG8182, GalNAc-T1 |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pharate adult |
| Map Viewer |
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Reference
[Please submit your publication]
|
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCTGCCTCGGTTCCGTTCC |
| Primer Seq. 3' |
aatctagaggtaccCTCAGCAGCACGGAGTAGGGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGCTGCCTC GGTTCCGTTC CTTTTACGGC AAACTGATCA TCTTCATCCT AGTCGCCCTC
0061 TGCTTCATCC TCTACAGCAA GGTGCAGCAG AATGGTTCAC CAGAGGAGCC ACCTGTNGCG
0121 CCACTCGTCC GGGCGGCCGC TCTGCGAGGT CATGGGCGCG AGCGGTTCGA GGCGTATAGC
0181 GACAGTGAGA ACGAGATAGC CCGACCCGCC ACCCAGTCTC CGTATGAACA AATAATCCAA
0241 CTGGATCTTC AGAAACAGAA GGTGGGACTC GGCGAACAGG GCGTGGCAGT GCATCTTTCC
0301 GGTGCCGCCA AGGAACGAGG CGATGAGATC TACAAGAAGA TTGCCCTCAA CGAGGAGCTA
0361 AGCGAGCAGT TGACCTACAA CCGGAGTGTC GGTGACCACC GGAATCCCCT GTGCGCCAAA
0421 CAGCGCTTTG ATTCCGACTC CCTGCCCACC GCCAGTGTGG TCATCATCTT CTTCAACGAA
0481 CCCTACTCCG TGCTGCTGAG |
| in silico PCR Fragment |
0001 atgctgcctc ggttccgttc cttttacggc aaactgatca tcttcatcct agtcgccctc
0061 tgcttcatcc tctacagcaa ggtgcagcag aatggttcac cagaggagcc acctgtagcg
0121 ccactcgtcc gggcggccgc tctgcgaggt catgggcgcg agcggttcga ggcgtatagc
0181 gacagtgaga acgagatagc ccgacccgcc acccagtcgc cgtacgaaca aataatccaa
0241 ctggatcttc agaaacagaa ggtgggactc ggcgaacagg gcgtggcagt gcatctttcc
0301 ggtgccgcca aggaacgagg cgatgagatc tacaagaaga ttgccctcaa cgaggagcta
0361 agcgagcagt tgacctacaa ccggagtgtc ggtgaccacc ggaatcccct gtgcgccaaa
0421 cagcgctttg attccgactc cctgcccacc gccagtgtgg tcatcatctt cttcaacgaa
0481 ccctactccg tgctgctgag |
| Assemble Data |
8182R-1.IR full 1 ATGCTGCCTCGGTTCCGTTCCTTTTACGGCAAACTGATCATCTTCATCCTAGTCGCCCTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 1 ATGCTGCCTCGGTTCCGTTCCTTTTACGGCAAACTGATCATCTTCATCCTAGTCGCCCTC 60
8182R-1.IR full 61 TGCTTCATCCTCTACAGCAAGGTGCAGCAGAATGGTTCACCAGAGGAGCCACCTGTNGCG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
8182R-1.in silico 61 TGCTTCATCCTCTACAGCAAGGTGCAGCAGAATGGTTCACCAGAGGAGCCACCTGTAGCG 120
8182R-1.IR full 121 CCACTCGTCCGGGCGGCCGCTCTGCGAGGTCATGGGCGCGAGCGGTTCGAGGCGTATAGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 121 CCACTCGTCCGGGCGGCCGCTCTGCGAGGTCATGGGCGCGAGCGGTTCGAGGCGTATAGC 180
8182R-1.IR full 181 GACAGTGAGAACGAGATAGCCCGACCCGCCACCCAGTCTCCGTATGAACAAATAATCCAA 240
|||||||||||||||||||||||||||||||||||||| ||||| |||||||||||||||
8182R-1.in silico 181 GACAGTGAGAACGAGATAGCCCGACCCGCCACCCAGTCGCCGTACGAACAAATAATCCAA 240
8182R-1.IR full 241 CTGGATCTTCAGAAACAGAAGGTGGGACTCGGCGAACAGGGCGTGGCAGTGCATCTTTCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 241 CTGGATCTTCAGAAACAGAAGGTGGGACTCGGCGAACAGGGCGTGGCAGTGCATCTTTCC 300
8182R-1.IR full 301 GGTGCCGCCAAGGAACGAGGCGATGAGATCTACAAGAAGATTGCCCTCAACGAGGAGCTA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 301 GGTGCCGCCAAGGAACGAGGCGATGAGATCTACAAGAAGATTGCCCTCAACGAGGAGCTA 360
8182R-1.IR full 361 AGCGAGCAGTTGACCTACAACCGGAGTGTCGGTGACCACCGGAATCCCCTGTGCGCCAAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 361 AGCGAGCAGTTGACCTACAACCGGAGTGTCGGTGACCACCGGAATCCCCTGTGCGCCAAA 420
8182R-1.IR full 421 CAGCGCTTTGATTCCGACTCCCTGCCCACCGCCAGTGTGGTCATCATCTTCTTCAACGAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8182R-1.in silico 421 CAGCGCTTTGATTCCGACTCCCTGCCCACCGCCAGTGTGGTCATCATCTTCTTCAACGAA 480
8182R-1.IR full 481 CCCTACTCCGTGCTGCTGAG 500
||||||||||||||||||||
8182R-1.in silico 481 CCCTACTCCGTGCTGCTGAG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137199.2 |
GalNAc-T1 CG8182-RA, transcript variant A (GalNAc-T1), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_166099.1 |
GalNAc-T1 CG8182-RB, transcript variant B (GalNAc-T1), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_133012.2 |
CG32560-RA (CG32560), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_135228.1 |
CG11321-RA, transcript variant A (CG11321), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_164709.1 |
CG11321-RB, transcript variant B (CG11321), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_140518.1 |
Argonaute 2 CG7439-RB, transcript variant B (AGO2), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168626.1 |
Argonaute 2 CG7439-RC, transcript variant C (AGO2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_140472.1 |
CG13466-RA (CG13466), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057242.3 |
Kinesin heavy chain CG7765-RA (Khc), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_143343.2 |
CG4963-RA (CG4963), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_134501.1 |
CG14227-RA (CG14227), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_140067.1 |
CG3654-RD (CG3654), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079108.2 |
benign gonial cell neoplasm CG30170-RA (bgcn), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001038869.1 |
CG33958-RA (CG33958), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140333.2 |
CG4069-RA (CG4069), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142663.3 |
CG15695-RA (CG15695), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_133011.2 |
CG8173-RA (CG8173), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079824.2 |
Dopamine receptor 2 CG18741-RB, transcript variant B (DopR2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_170420.1 |
Dopamine receptor 2 CG18741-RA, transcript variant A (DopR2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079671.2 |
stripe CG7847-RA, transcript variant A (sr), mRNA |
| 0 |
0 |
1 |
1 |
3 |
NM_136739.1 |
CG11825-RA (CG11825), mRNA |
| 0 |
0 |
0 |
6 |
15 |
NM_139492.2 |
polypeptide GalNAc transferase 6 CG2103-RA, transcript variant A (pgant6), mRNA |
| 0 |
0 |
0 |
6 |
15 |
NM_167966.1 |
polypeptide GalNAc transferase 6 CG2103-RB, transcript variant B (pgant6), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_164539.1 |
polypeptide GalNAc transferase 4 CG31956-RA (pgant4), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_170281.1 |
CG5476-RA (CG5476), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_170279.2 |
CG6447-RA, transcript variant A (CG6447), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_170280.1 |
CG6447-RB, transcript variant B (CG6447), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_144077.2 |
CG18675-RA (CG18675), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132239.1 |
CG1632-RA (CG1632), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_057401.3 |
aurora CG3068-RA (aur), mRNA |
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