NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8151R-1

Symbol Tfb1 Full Name Tfb1

CG No CG8151 Old CG No CG8151

Synonyms TFB1, CG8151, l(2)06949, Dmp62, l(2)06950, Tfb1

Accession No (Link to NCBI) NM_166048.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTACAAGAAGGGCGACGGCAC

Primer Seq. 3'

aatctagaggtaccAGCCGTCTGTCTGCGGCTTTA

Predicted Fragment Size

IR fragment full Seq

0001 GTACAAGAAG GGCGACGGCA CGCTCTACGT AATGAATGAG CGTGTGGCCT GGATGGCGGA 
0061 ACACCGGGAC ACGGNAACAG TCTCCCATCG TTATGCGGAT ATCAAGACTC AAAAGATATC 
0121 TCCTGAGGGC AAGCCCAAGG TGCAGCTGCA AGTGGTTCTT CACGACGGCA ACACATCGAC 
0181 CTTCCACTTC GTCAACCGCC AGGGACAGGC CGCAATGCTT GCCGACAGGG ATCAAGGTCA 
0241 AGGAGCTATT GCAGCAACTG CTTCCCAACT TCAAGCGGAA GGTGGACAAA GACCTGGAAG 
0301 ACAAGAACCG CATCCTTGTT GAGAATCCCA ACCTGCTGCA ACTCTACAAG GACCTTGTCA 
0361 TAACCAAAGT CCTAACCAGC GATGAGTTCT ggGCTGACGC ATGCCAAGGA TCACGCCCTT 
0421 AAGAAAATGG GCAGATCCCA GGAGATCGGT GTTTCTGGCG CCTTTCTGGC TGACATAAAG 
0481 CCGCAGACAG ACGGCT

in silico PCR Fragment

0001 GTACAAGAAG GGCGACGGCA CGCTCTACGT AATGAATGAG CGTGTGGCCT GGATGGCGGA 
0061 ACACCGGGAC ACGGTAACAG TCTCCCATCG TTATGCGGAT ATCAAGACTC AAAAGATATC 
0121 TCCTGAGGGC AAGCCCAAGG TGCAGCTGCA AGTGGTTCTT CACGACGGCA ACACATCGAC 
0181 CTTCCACTTC GTCAACCGCC AGGGACAGGC CGCAATGCTT GCCGACAGGG A-CAAGGTCA 
0241 AGGAGCTATT GCAGCAACTG CTTCCCAACT TCAAGCGGAA GGTGGACAAA GACCTGGAAG 
0301 ACAAGAACCG CATCCTTGTT GAGAATCCCA ACCTGCTGCA ACTCTACAAG GACCTTGTCA 
0361 TAACCAAAGT CCTAACCAGC GATGAGTTCT GGGCT-ACGC ATGCCAAGGA TCACGCCCTT 
0421 AAGAAAATGG GCAGATCCCA GGAGATCGGT GTTTCTGGCG CCTTTCTGGC TGACATAAAG 
0481 CCGCAGACAG ACGGCT

Assemble Data


8151R-1.IR_full       1   GTACAAGAAGGGCGACGGCACGCTCTACGTAATGAATGAGCGTGTGGCCTGGATGGCGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     1   GTACAAGAAGGGCGACGGCACGCTCTACGTAATGAATGAGCGTGTGGCCTGGATGGCGGA 60


8151R-1.IR_full       61  ACACCGGGACACGGNAACAGTCTCCCATCGTTATGCGGATATCAAGACTCAAAAGATATC 120
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     61  ACACCGGGACACGGTAACAGTCTCCCATCGTTATGCGGATATCAAGACTCAAAAGATATC 120


8151R-1.IR_full       121 TCCTGAGGGCAAGCCCAAGGTGCAGCTGCAAGTGGTTCTTCACGACGGCAACACATCGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     121 TCCTGAGGGCAAGCCCAAGGTGCAGCTGCAAGTGGTTCTTCACGACGGCAACACATCGAC 180


8151R-1.IR_full       181 CTTCCACTTCGTCAACCGCCAGGGACAGGCCGCAATGCTTGCCGACAGGGATCAAGGTCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
8151R-1.in silico     181 CTTCCACTTCGTCAACCGCCAGGGACAGGCCGCAATGCTTGCCGACAGGGA-CAAGGTCA 240


8151R-1.IR_full       241 AGGAGCTATTGCAGCAACTGCTTCCCAACTTCAAGCGGAAGGTGGACAAAGACCTGGAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     241 AGGAGCTATTGCAGCAACTGCTTCCCAACTTCAAGCGGAAGGTGGACAAAGACCTGGAAG 300


8151R-1.IR_full       301 ACAAGAACCGCATCCTTGTTGAGAATCCCAACCTGCTGCAACTCTACAAGGACCTTGTCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     301 ACAAGAACCGCATCCTTGTTGAGAATCCCAACCTGCTGCAACTCTACAAGGACCTTGTCA 360


8151R-1.IR_full       361 TAACCAAAGTCCTAACCAGCGATGAGTTCTGGGCTGACGCATGCCAAGGATCACGCCCTT 420
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
8151R-1.in silico     361 TAACCAAAGTCCTAACCAGCGATGAGTTCTGGGCT-ACGCATGCCAAGGATCACGCCCTT 420


8151R-1.IR_full       421 AAGAAAATGGGCAGATCCCAGGAGATCGGTGTTTCTGGCGCCTTTCTGGCTGACATAAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8151R-1.in silico     421 AAGAAAATGGGCAGATCCCAGGAGATCGGTGTTTCTGGCGCCTTTCTGGCTGACATAAAG 480


8151R-1.IR_full       481 CCGCAGACAGACGGCT 496
                          ||||||||||||||||
8151R-1.in silico     481 CCGCAGACAGACGGCT 496

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	476	0	0	0	NM_137113.2	CG8151-RB, transcript variant B (Tfb1), mRNA
100	476	0	0	0	NM_166048.1	CG8151-RA, transcript variant A (Tfb1), mRNA
100	476	0	0	0	NM_166049.1	CG8151-RC, transcript variant C (Tfb1), mRNA
0	0	3	2	2	NM_168980.1	CG11100-RC, transcript variant C (Mes2), mRNA
0	0	3	2	2	NM_168979.2	CG11100-RB, transcript variant B (Mes2), mRNA
0	0	3	2	2	NM_130488.2	CG3156-RA (CG3156), mRNA
0	0	2	4	6	NM_136000.2	CG6453-RA (CG6453), mRNA
0	0	1	8	5	NM_140020.2	CG5068-RA (CG5068), mRNA
0	0	1	5	7	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	1	3	8	NM_142524.2	CG6040-RA (CG6040), mRNA
0	0	1	3	3	NM_001043036.1	CG17800-PAA (Dscam), mRNA
0	0	1	3	2	NM_206138.1	CG15920-RB, transcript variant B (resilin), mRNA
0	0	1	3	2	NM_137313.2	CG15920-RA, transcript variant A (resilin), mRNA
0	0	1	3	2	NM_142805.3	CG6921-RB, transcript variant B (CG6921), mRNA
0	0	1	3	2	NM_170022.1	CG6921-RA, transcript variant A (CG6921), mRNA
0	0	1	3	2	NM_170023.1	CG6921-RC, transcript variant C (CG6921), mRNA
0	0	1	2	5	NM_176302.1	CG6282-RB, transcript variant B (CG6282), mRNA
0	0	1	2	5	NM_139986.2	CG6282-RA, transcript variant A (CG6282), mRNA
0	0	1	2	4	NM_135222.2	CG11188-RA (CG11188), mRNA
0	0	1	2	2	NM_164716.1	CG31632-RA (CG31632), mRNA
0	0	0	7	8	NM_168387.1	CG6711-RA (Taf2), mRNA
0	0	0	6	4	NM_166671.1	CG30164-RA (CG30164), mRNA
0	0	0	5	4	NM_080257.2	CG6815-RA (bor), mRNA
0	0	0	5	3	NM_001032265.1	CG4844-RA, transcript variant A (CG4844), mRNA
0	0	0	5	3	NM_001032264.1	CG4844-RB, transcript variant B (CG4844), mRNA
0	0	0	5	3	NM_001032266.1	CG18431-RA, transcript variant A (CG18431), mRNA
0	0	0	5	2	NM_167449.1	CG6340-RD, transcript variant D (CG6340), mRNA
0	0	0	5	2	NM_132806.2	CG6340-RB, transcript variant B (CG6340), mRNA
0	0	0	5	2	NM_132926.1	CG13008-RA, transcript variant A (CG13008), mRNA
0	0	0	5	2	NM_167537.1	CG13008-RB, transcript variant B (CG13008), mRNA

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