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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8056R-3 |
| Symbol |
Krn |
Full Name |
Keren |
| CG No |
CG32179 |
Old CG No |
CG8056 |
| Synonyms |
keren/spitz2, keren, Ker, CT19073, gritz, CG8056, CG32179, Krn, s-krn |
| Accession No (Link to NCBI) |
NM_079405.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Zhang P, Holowatyj AN, Roy T, Pronovost SM, Marchetti M, Liu H, Ulrich CM, Edgar BA.
An SH3PX1-Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling.
Dev Cell (2019) 49(4) 574-589.e5
[
PubMed ID = 31006650
]
[
RRC reference
]
Duncan OF, Granat L, Ranganathan R, Singh VK, Mazaud D, Fanto M, Chambers D, Ballard CG, Bateman JM.
Ras-ERK-ETS inhibition alleviates neuronal mitochondrial dysfunction by reprogramming mitochondrial retrograde signaling.
PLoS Genet (2018) 14(7) e1007567
[
PubMed ID = 30059502
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Xu N, Wang SQ, Tan D, Gao Y, Lin G, Xi R.
EGFR, Wingless and JAK/STAT signaling cooperatively maintain Drosophila intestinal stem cells.
Dev Biol (2011) 354(1) 31-43
[
PubMed ID = 21440535
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGATCTGCTGCTGCTTGCCAC |
| Primer Seq. 3' |
aatctagaggtaccCCTCGTTCTGACAGCCACCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGATCTGCTG CTGCTTGCCA CCGCTTTAAT CGGCGCTTAC CTACCGCTCA CCGCCGCCTG
0061 TTCCTCGCGT GCCATCGCTA AGCCGCGGCC AACAGCTGCA CCGATCCTGC CGCCGGATAA
0121 TGTGGAAATA TCCACAACGC CGAGACCGAA TGTCACCTTC CCGATCTTCG CCTGTCCACC
0181 GACCTATGTC GCCTGGTATT GCCTGAACGA TGGCACCTGC TTTACGGTGA AGATCCACAA
0241 TGAAATCCTA TACAACTGCG AATGTGCGCT GGGATTTATG GGTCCGAGGT GCGAGTACAA
0301 GGAGATTGAT GGCTCGTACC TGCCAACCAG GAACCGTGTG ATGCTAGAGA AGGCCAGCAT
0361 TGTTAGCGGA GCAACGCTGG CCCTTCTGTT TATGGCCATG TGCTGTGTGG TTTTGTATCT
0421 GCGACACGAG AAGCTGCAAA AACAAAAGCT GCACGACAGC ACCACAACCA CAACGACCGA
0481 CGGTGGCTGT CAGAACGAGG |
| in silico PCR Fragment |
0001 GGATCTGCTG CTGCTTGCCA CCGCTTTAAT CGGCGCTTAC CTACCGCTCA CCGCCGCCTG
0061 TTCCTCGCGT GCCATCGCTA AGCCGCGGCC AACAGCTGCA CCGATCCTGC CGCCGGATAA
0121 TGTGGAAATA TCCACAACGC CGAGACCGAA TGTCACCTTC CCGATCTTCG CCTGTCCACC
0181 GACCTATGTC GCCTGGTATT GCCTGAACGA TGGCACCTGC TTTACGGTGA AGATCCACAA
0241 TGAAATCCTA TACAACTGCG AATGTGCGCT GGGATTTATG GGTCCGAGGT GCGAGTACAA
0301 GGAGATTGAT GGCTCGTACC TGCCAACCAG GAACCGTGTG ATGCTAGAGA AGGCCAGCAT
0361 TGTTAGCGGA GCAACGCTGG CCCTTCTGTT TATGGCCATG TGCTGTGTGG TTTTGTATCT
0421 GCGACACGAG AAGCTGCAAA AACAAAAGCT GCACGACAGC ACCACAACCA CAACGACCGA
0481 CGGTGGCTGT CAGAACGAGG |
| Assemble Data |
8056R-3.IR_full 1 GGATCTGCTGCTGCTTGCCACCGCTTTAATCGGCGCTTACCTACCGCTCACCGCCGCCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 1 GGATCTGCTGCTGCTTGCCACCGCTTTAATCGGCGCTTACCTACCGCTCACCGCCGCCTG 60
8056R-3.IR_full 61 TTCCTCGCGTGCCATCGCTAAGCCGCGGCCAACAGCTGCACCGATCCTGCCGCCGGATAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 61 TTCCTCGCGTGCCATCGCTAAGCCGCGGCCAACAGCTGCACCGATCCTGCCGCCGGATAA 120
8056R-3.IR_full 121 TGTGGAAATATCCACAACGCCGAGACCGAATGTCACCTTCCCGATCTTCGCCTGTCCACC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 121 TGTGGAAATATCCACAACGCCGAGACCGAATGTCACCTTCCCGATCTTCGCCTGTCCACC 180
8056R-3.IR_full 181 GACCTATGTCGCCTGGTATTGCCTGAACGATGGCACCTGCTTTACGGTGAAGATCCACAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 181 GACCTATGTCGCCTGGTATTGCCTGAACGATGGCACCTGCTTTACGGTGAAGATCCACAA 240
8056R-3.IR_full 241 TGAAATCCTATACAACTGCGAATGTGCGCTGGGATTTATGGGTCCGAGGTGCGAGTACAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 241 TGAAATCCTATACAACTGCGAATGTGCGCTGGGATTTATGGGTCCGAGGTGCGAGTACAA 300
8056R-3.IR_full 301 GGAGATTGATGGCTCGTACCTGCCAACCAGGAACCGTGTGATGCTAGAGAAGGCCAGCAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 301 GGAGATTGATGGCTCGTACCTGCCAACCAGGAACCGTGTGATGCTAGAGAAGGCCAGCAT 360
8056R-3.IR_full 361 TGTTAGCGGAGCAACGCTGGCCCTTCTGTTTATGGCCATGTGCTGTGTGGTTTTGTATCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 361 TGTTAGCGGAGCAACGCTGGCCCTTCTGTTTATGGCCATGTGCTGTGTGGTTTTGTATCT 420
8056R-3.IR_full 421 GCGACACGAGAAGCTGCAAAAACAAAAGCTGCACGACAGCACCACAACCACAACGACCGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8056R-3.in silico 421 GCGACACGAGAAGCTGCAAAAACAAAAGCTGCACGACAGCACCACAACCACAACGACCGA 480
8056R-3.IR_full 481 CGGTGGCTGTCAGAACGAGG 500
||||||||||||||||||||
8056R-3.in silico 481 CGGTGGCTGTCAGAACGAGG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079405.3 |
CG32179-RA (Krn), mRNA |
| 0 |
0 |
3 |
3 |
13 |
NM_132912.1 |
CG4521-RA (mthl1), mRNA |
| 0 |
0 |
3 |
2 |
16 |
NM_169453.1 |
CG10095-RA (dpr15), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_132932.2 |
CG9634-RA (CG9634), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136347.1 |
CG14471-RB, transcript variant B (CG14471), mRNA |
| 0 |
0 |
1 |
9 |
8 |
NM_206324.1 |
CG33267-RA (CG33267), mRNA |
| 0 |
0 |
1 |
4 |
19 |
NM_205928.1 |
CG8086-RD, transcript variant D (CG8086), mRNA |
| 0 |
0 |
1 |
4 |
19 |
NM_205929.1 |
CG8086-RC, transcript variant C (CG8086), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_142903.2 |
CG10183-RA (CG10183), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_137672.1 |
CG15225-RA (CG15225), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_135892.2 |
CG4185-RA (NC2beta), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001014642.1 |
CG33518-RA (mun), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132086.1 |
CG15894-RA (CG15894), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_137238.2 |
CG8403-RA (SP2353), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001042904.1 |
CG15148-RB, transcript variant B (btv), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001042905.1 |
CG15148-RC, transcript variant C (btv), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136013.1 |
CG15148-RA, transcript variant A (btv), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140476.2 |
CG5392-RA (CG5392), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132793.2 |
CG9164-RA, transcript variant A (CG9164), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167440.1 |
CG9164-RC, transcript variant C (CG9164), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080529.2 |
CG9854-RA, transcript variant A (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166372.1 |
CG9854-RB, transcript variant B (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043097.1 |
CG9854-RC, transcript variant C (hrg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136177.2 |
CG10730-RA (CG10730), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_133004.1 |
CG8211-RA (CG8211), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132428.1 |
CG17333-RA (CG17333), mRNA |
| 0 |
0 |
1 |
1 |
2 |
NM_140735.1 |
CG6311-RB (CG6311), mRNA |
| 0 |
0 |
0 |
45 |
70 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
0 |
20 |
119 |
NM_170227.2 |
CG31439-RA (CG31439), mRNA |
| 0 |
0 |
0 |
8 |
5 |
NM_001043241.1 |
CG14713-RB (CG14713), mRNA |
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