NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7971R-2

Symbol CG7971 Full Name CG7971

CG No CG7971 Old CG No CG7971

Synonyms unnamed, anon-EST:fe1A7, BcDNA:GH04236, CG7971, anon-fast-evolving-1A7

Accession No (Link to NCBI) NM_139385.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGGACAGGGCAAAACAGAT

Primer Seq. 3'

aatctagaggtaccTGCGCGACCTTTTCTTCTTTG

Predicted Fragment Size

IR fragment full Seq

0001 ATGGGACAGG GCAAAACAGA TCTGGCCAAA GATGAATTCG GACGCGTAgc TGNCTCGCGA 
0061 CACCCATCAA ATAGCCGAGG CTCAACAGCA GAAGAACGCC AGGCTGCGCG AGGCCTTCAA 
0121 CATATCCGAG TACTTTGTCG AGGGCAGCAG CNTTCGACGG TGATCGCAAG GCGAAGGAGG 
0181 ACCTGGCCAA AAGCGTGGCT CTCCAAAAGG AACTGGACGC CCAGCGCGAG AGcCTGGCAG 
0241 CGGCGGCAGc TGCCGCAGCN AGCCGGCANA GGACNAAGGA GACCGGGAAN GCGGTATGCC 
0301 CTAGTGCGTA CTCCTTCCCG CGAACGGGAT CGCGATGCTG GCGATGCGGC AGCCAATGGC 
0361 GACGAACGCG ATCATGTCTC CAAGACGGAC AAAAAGAAGC GCAAGAAGCG CGCCAGAGAG 
0421 AGTTCGGCCA GTCCTGAGCG CAAGAAGAAG AAGAAGTCGA AGAAGCACAA GAAAGAGAGT 
0481 AAGTCAAAGA AGAAAAGGTC GCGCA

in silico PCR Fragment

0001 ATGGGACAGG GCAAAACAGA TCTGGCCAAA GATGAATTCG GACGCGTAGC TG-CTCGCGA 
0061 CACCCATCAA ATAGCCGAGG CTCAACAGCA GAAGAACGCC AGGCTGCGCG AGGCCTTCAA 
0121 CATATCCGAG TACTTTGTCG AGGGCAGCAG C-TTCGACGG TGATCGCAAG GCGAAGGAGG 
0181 ACCTGGCCAA AAGCGTGGCT CTCCAAAAGG AACTGGACGC CCAGCGCGAG AGCCTGGCAG 
0241 CGGCGGCAGC TGCCGCAGC- AGCCGGCA-A GGAC-AAGGA GACCGGGAA- GCGGTATGCC 
0301 CTAGTGCGTA CTCCTTCCCG CGAACGGGAT CGCGATGCTG GCGATGCGGC AGCCAATGGC 
0361 GACGAACGCG ATCATGTCTC CAAGACGGAC AAGAAGAAGC GCAAGAAGCG CGCCAGAGAG 
0421 AGTTCGGCCA GTCCTGAGCG CAAGAAGAAG AAGAAGTCGA AGAAGCACAA GAAAGAGAGT 
0481 AAGTCAAAGA AGAAAAGGTC GCGCA

Assemble Data


7971R-2.IR_full       1   ATGGGACAGGGCAAAACAGATCTGGCCAAAGATGAATTCGGACGCGTAGCTGNCTCGCGA 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
7971R-2.in silico     1   ATGGGACAGGGCAAAACAGATCTGGCCAAAGATGAATTCGGACGCGTAGCTG-CTCGCGA 60


7971R-2.IR_full       61  CACCCATCAAATAGCCGAGGCTCAACAGCAGAAGAACGCCAGGCTGCGCGAGGCCTTCAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7971R-2.in silico     61  CACCCATCAAATAGCCGAGGCTCAACAGCAGAAGAACGCCAGGCTGCGCGAGGCCTTCAA 120


7971R-2.IR_full       121 CATATCCGAGTACTTTGTCGAGGGCAGCAGCNTTCGACGGTGATCGCAAGGCGAAGGAGG 180
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
7971R-2.in silico     121 CATATCCGAGTACTTTGTCGAGGGCAGCAGC-TTCGACGGTGATCGCAAGGCGAAGGAGG 180


7971R-2.IR_full       181 ACCTGGCCAAAAGCGTGGCTCTCCAAAAGGAACTGGACGCCCAGCGCGAGAGCCTGGCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7971R-2.in silico     181 ACCTGGCCAAAAGCGTGGCTCTCCAAAAGGAACTGGACGCCCAGCGCGAGAGCCTGGCAG 240


7971R-2.IR_full       241 CGGCGGCAGCTGCCGCAGCNAGCCGGCANAGGACNAAGGAGACCGGGAANGCGGTATGCC 300
                          ||||||||||||||||||| |||||||| ||||| |||||||||||||| ||||||||||
7971R-2.in silico     241 CGGCGGCAGCTGCCGCAGC-AGCCGGCA-AGGAC-AAGGAGACCGGGAA-GCGGTATGCC 300


7971R-2.IR_full       301 CTAGTGCGTACTCCTTCCCGCGAACGGGATCGCGATGCTGGCGATGCGGCAGCCAATGGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7971R-2.in silico     301 CTAGTGCGTACTCCTTCCCGCGAACGGGATCGCGATGCTGGCGATGCGGCAGCCAATGGC 360


7971R-2.IR_full       361 GACGAACGCGATCATGTCTCCAAGACGGACAAAAAGAAGCGCAAGAAGCGCGCCAGAGAG 420
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
7971R-2.in silico     361 GACGAACGCGATCATGTCTCCAAGACGGACAAGAAGAAGCGCAAGAAGCGCGCCAGAGAG 420


7971R-2.IR_full       421 AGTTCGGCCAGTCCTGAGCGCAAGAAGAAGAAGAAGTCGAAGAAGCACAAGAAAGAGAGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7971R-2.in silico     421 AGTTCGGCCAGTCCTGAGCGCAAGAAGAAGAAGAAGTCGAAGAAGCACAAGAAAGAGAGT 480


7971R-2.IR_full       481 AAGTCAAAGAAGAAAAGGTCGCGCA 505
                          |||||||||||||||||||||||||
7971R-2.in silico     481 AAGTCAAAGAAGAAAAGGTCGCGCA 505

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	481	0	10	33	NM_001031932.1	CG7971-RD, transcript variant D (CG7971), mRNA
100	481	0	10	33	NM_139385.3	CG7971-RA, transcript variant A (CG7971), mRNA
100	481	0	10	33	NM_167909.2	CG7971-RC, transcript variant C (CG7971), mRNA
94.59	455	1	10	17	NM_167910.2	CG7971-RB, transcript variant B (CG7971), mRNA
0.83	4	5	12	94	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0.83	4	5	12	94	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0.83	4	2	14	46	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0.2	1	6	18	102	NM_079671.2	CG7847-RA, transcript variant A (sr), mRNA
0.2	1	6	18	94	NM_169786.1	CG7847-RB, transcript variant B (sr), mRNA
0.2	1	5	19	64	NM_167387.1	CG32611-RB (CG32611), mRNA
0.2	1	5	10	84	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
0.2	1	5	10	83	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA
0.2	1	5	10	83	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA
0.2	1	5	10	73	NM_169260.1	CG9755-RB, transcript variant B (pum), mRNA
0.2	1	5	10	73	NM_176427.1	CG9755-RE, transcript variant E (pum), mRNA
0.2	1	5	9	11	NM_165425.2	CG2682-RB, transcript variant B (d4), mRNA
0.2	1	5	9	11	NM_136319.3	CG2682-RA, transcript variant A (d4), mRNA
0.2	1	5	9	11	NM_165424.2	CG2682-RC, transcript variant C (d4), mRNA
0.2	1	4	5	9	NM_206288.1	CG33277-RA (CG33277), mRNA
0.2	1	3	16	22	NM_143402.2	CG1420-RA (CG1420), mRNA
0.2	1	2	5	2	NM_079297.2	CG6502-RA (E(z)), mRNA
0.2	1	2	2	40	NM_206303.1	CG6964-RD, transcript variant D (Gug), mRNA
0.2	1	2	2	38	NM_176300.1	CG6964-RC, transcript variant C (Gug), mRNA
0.2	1	2	2	38	NM_079249.2	CG6964-RB, transcript variant B (Gug), mRNA
0.2	1	2	2	38	NM_145106.1	CG6964-RA, transcript variant A (Gug), mRNA
0	0	11	5	9	NM_206492.1	CG33332-RA (CG33332), mRNA
0	0	9	3	18	NM_167608.1	CG7178-RG, transcript variant G (wupA), mRNA
0	0	7	14	46	NM_132246.2	CG10555-RA (CG10555), mRNA
0	0	7	3	11	NM_143223.1	CG14238-RA (CG14238), mRNA
0	0	6	9	49	NM_168037.1	CG32264-RA, transcript variant A (CG32264), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance
++

Back