|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7923R-2 |
| Symbol |
Fad2 |
Full Name |
Fad2 |
| CG No |
CG7923 |
Old CG No |
CG7923 |
| Synonyms |
fad2, CG7923, fad, Fad2 |
| Accession No (Link to NCBI) |
NM_143709.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGAATCCGATGTCGAGACAGT |
| Primer Seq. 3' |
aatctagaggtaccGATCTGCCTCCAGATCGGATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGAATCCGAT GTCGAGACAG TAGACGGTGG ATTGTCGACG GAGCTAAGCA ATCGGAAGAC
0061 CACCGATGGA AGTAAACTGG AGTTGGTATG GGTCAATATA GTGCTGTTCG TGATCCTTCA
0121 CATATCTTCG CTATATGGAG TCTGGCTGCT CTTCACTTCG GCCACGTGGA CAACGGTCGT
0181 GCTCTTTTGG CCAACGGTTG TGGTGACCAT TTTGGGAGTT TCCGGCGGTG CCCATCGTTT
0241 GTgGGCTCAT CGCACATTCA AGGCGAATAC ANCCGCTCAA GCTGATCTTT TTGTTCCTGA
0301 ACACTTTGGC CTTCCAGGAT GCGGTCTACT ATTGGGCCAG GGATCATCGC GTCCACCACA
0361 AGTATACGGA AACTGATGCG GATCCGTACA ATTCGCAACG TGGTTGGTTC TTTGCCCACA
0421 TCGGTTGGTt gTGCTGCCGC AAGCATCCGG AGGTTGTGGA GAAGGGCAAG CAAATAGATC
0481 TATNCGATCT GGAGGCAGAT C |
| in silico PCR Fragment |
0001 CGAATCCGAT GTCGAGACAG TAGACGGTGG ATTGTCGACG GAGCTAAGCA ATCGGAAGAC
0061 CACCGATGGA AGTAAACTGG AGTTGGTATG GGTCAATATA GTGCTGTTCG TGATCCTTCA
0121 CATATCTTCG CTATATGGAG TCTGGCTGCT CTTCACTTCG GCCACGTGGA CAACGGTCGT
0181 GCTCTTTTGG CCAACGGTTG TGGTGACCAT TTTGGGAGTT TCCGGCGGTG CCCATCGTTT
0241 GTGGGCTCAT CGCACATTCA AGGCGAATAC A-CCGCTCAA GCTGATCTTT TTGTTCCTGA
0301 ACACTTTGGC CTTTCAGGAT GCGGTCTACT ATTGGGCCCG GGATCATCGC GTCCACCACA
0361 AGTATACGGA AACTGATGCG GATCCGTACA ATTCGCAACG TGGTTGGTTC TTTGCCCACA
0421 TCGGTTGGTT GTGCTGCCGC AAGCATCCGG AGGTTGTGGA GAAGGGCAAG CAAATAGATC
0481 TATCCGATCT GGAGGCAGAT C |
| Assemble Data |
7923R-2.IR_full 1 CGAATCCGATGTCGAGACAGTAGACGGTGGATTGTCGACGGAGCTAAGCAATCGGAAGAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 1 CGAATCCGATGTCGAGACAGTAGACGGTGGATTGTCGACGGAGCTAAGCAATCGGAAGAC 60
7923R-2.IR_full 61 CACCGATGGAAGTAAACTGGAGTTGGTATGGGTCAATATAGTGCTGTTCGTGATCCTTCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 61 CACCGATGGAAGTAAACTGGAGTTGGTATGGGTCAATATAGTGCTGTTCGTGATCCTTCA 120
7923R-2.IR_full 121 CATATCTTCGCTATATGGAGTCTGGCTGCTCTTCACTTCGGCCACGTGGACAACGGTCGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 121 CATATCTTCGCTATATGGAGTCTGGCTGCTCTTCACTTCGGCCACGTGGACAACGGTCGT 180
7923R-2.IR_full 181 GCTCTTTTGGCCAACGGTTGTGGTGACCATTTTGGGAGTTTCCGGCGGTGCCCATCGTTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 181 GCTCTTTTGGCCAACGGTTGTGGTGACCATTTTGGGAGTTTCCGGCGGTGCCCATCGTTT 240
7923R-2.IR_full 241 GTGGGCTCATCGCACATTCAAGGCGAATACANCCGCTCAAGCTGATCTTTTTGTTCCTGA 300
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
7923R-2.in silico 241 GTGGGCTCATCGCACATTCAAGGCGAATACA-CCGCTCAAGCTGATCTTTTTGTTCCTGA 300
7923R-2.IR_full 301 ACACTTTGGCCTTCCAGGATGCGGTCTACTATTGGGCCAGGGATCATCGCGTCCACCACA 360
||||||||||||| |||||||||||||||||||||||| |||||||||||||||||||||
7923R-2.in silico 301 ACACTTTGGCCTTTCAGGATGCGGTCTACTATTGGGCCCGGGATCATCGCGTCCACCACA 360
7923R-2.IR_full 361 AGTATACGGAAACTGATGCGGATCCGTACAATTCGCAACGTGGTTGGTTCTTTGCCCACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 361 AGTATACGGAAACTGATGCGGATCCGTACAATTCGCAACGTGGTTGGTTCTTTGCCCACA 420
7923R-2.IR_full 421 TCGGTTGGTTGTGCTGCCGCAAGCATCCGGAGGTTGTGGAGAAGGGCAAGCAAATAGATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7923R-2.in silico 421 TCGGTTGGTTGTGCTGCCGCAAGCATCCGGAGGTTGTGGAGAAGGGCAAGCAAATAGATC 480
7923R-2.IR_full 481 TATNCGATCTGGAGGCAGATC 501
||| |||||||||||||||||
7923R-2.in silico 481 TATCCGATCTGGAGGCAGATC 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143709.2 |
CG7923-RA (Fad2), mRNA |
| 1.03 |
5 |
2 |
2 |
3 |
NM_137750.1 |
CG10321-RA (CG10321), mRNA |
| 0 |
0 |
8 |
33 |
53 |
NM_141944.1 |
CG5925-RA (desat2), mRNA |
| 0 |
0 |
7 |
19 |
31 |
NM_169467.1 |
CG5887-RD, transcript variant D (desat1), mRNA |
| 0 |
0 |
7 |
19 |
31 |
NM_169465.1 |
CG5887-RB, transcript variant B (desat1), mRNA |
| 0 |
0 |
7 |
19 |
31 |
NM_169468.1 |
CG5887-RE, transcript variant E (desat1), mRNA |
| 0 |
0 |
7 |
19 |
31 |
NM_169466.1 |
CG5887-RC, transcript variant C (desat1), mRNA |
| 0 |
0 |
7 |
19 |
31 |
NM_144474.1 |
CG5887-RA, transcript variant A (desat1), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132376.1 |
CG2974-RA (CG2974), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_079114.1 |
CG11290-RA (enok), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_144370.2 |
CG17932-RA, transcript variant A (Ugt36Bc), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_165193.1 |
CG17932-RB, transcript variant B (Ugt36Bc), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_140979.1 |
CG18281-RA (CG18281), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_140980.1 |
CG17637-RA (CG17637), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137172.1 |
CG8090-RA (CG8090), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164317.2 |
CG1107-RA, transcript variant A (auxillin), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_139769.1 |
CG10467-RA (CG10467), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141181.2 |
CG1107-RB, transcript variant B (auxillin), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168387.1 |
CG6711-RA (Taf2), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_176041.1 |
CG33090-RB (CG33090), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_168665.1 |
CG13035-RB, transcript variant B (CG13035), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140635.1 |
CG13035-RA, transcript variant A (CG13035), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_136094.1 |
CG10493-RA (CG10493), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135621.1 |
CG12517-RA (CG12517), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164950.1 |
CG31723-RA (CG31723), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169842.1 |
CG31295-RA (CG31295), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_057646.2 |
CG10844-RD, transcript variant D (Rya-r44F), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_057645.2 |
CG10844-RC, transcript variant C (Rya-r44F), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_057644.2 |
CG10844-RB, transcript variant B (Rya-r44F), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_057492.3 |
CG4114-RA (ex), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| ++ |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
|
