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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7904R-2 |
| Symbol |
put |
Full Name |
punt |
| CG No |
CG7904 |
Old CG No |
CG7904 |
| Synonyms |
atrII, CG7904, Punt, TGF-B, Atr-II, l(3)10460, STK-C, pun, TGF-beta, l(3)j5A5, dlhC, Tgf-r, AtrII, Atr, Atr88CD, Act-r, put, Put |
| Accession No (Link to NCBI) |
NM_169591.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ostalé CM, Esteban N, López-Varea A, de Celis JF.
Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.
G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Ma H, Zhao H, Liu F, Zhao H, Kong R, Shi L, Wei M, Li Z.
Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut.
Biol Open (2019) 8(10)
[
PubMed ID = 31628141
]
[
RRC reference
]
Xu R, Li J, Zhao H, Kong R, Wei M, Shi L, Bai G, Li Z.
Self-restrained regulation of stem cell niche activity by niche components in the Drosophila testis.
Dev Biol (2018) 439(1) 42-51
[
PubMed ID = 29679558
]
[
RRC reference
]
Le V, Anderson E, Akiyama T, Wharton KA.
Drosophila models of FOP provide mechanistic insight.
Bone (2018) 109 192-200
[
PubMed ID = 29128351
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Le VQ, Wharton KA.
Hyperactive BMP signaling induced by ALK2(R206H) requires type II receptor function in a Drosophila model for classic fibrodysplasia ossificans progressiva.
Dev Dyn (2012) 241(1) 200-14
[
PubMed ID = 22174087
]
[
RRC reference
]
Boulanger A, Farge M, Ramanoudjame C, Wharton K, Dura JM.
Drosophila motor neuron retraction during metamorphosis is mediated by inputs from TGF-β/BMP signaling and orphan nuclear receptors.
PLoS One (2012) 7(7) e40255
[
PubMed ID = 22792255
]
[
RRC reference
]
Li Z, Zhang Y, Han L, Shi L, Lin X.
Trachea-derived dpp controls adult midgut homeostasis in Drosophila.
Dev Cell (2013) 24(2) 133-43
[
PubMed ID = 23369712
]
[
RRC reference
]
Hevia CF, de Celis JF.
Activation and function of TGFβ signalling during Drosophila wing development and its interactions with the BMP pathway.
Dev Biol (2013) 377(1) 138-53
[
PubMed ID = 23485686
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATCTGCTTTATCTAACGGCG |
| Primer Seq. 3' |
aatctagaggtaccAGAAGAAGGCCCATGCCAACA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GATCTGcTTT ATCTAACGGC GCAgcNAACG CTGGTCTGCT GTCTGATTGG AATCCATGGA
0061 TCTATTTTGC CCGGAAGTCA TGGGATCATA GAATGCGAGC ACTTCGACGA GAAGATGTGC
0121 AACACAACGC AGCAATGTGA AACACGGATA GAGCACTGTA AGATGGAGGC GGATAAGTTT
0181 CCCAGCTGCT ATGTCCTTTG GTCGGTCAAC GAGACAACGG GCATCCTGCG CATCAAGATG
0241 AAGGGCTGCT TCACGGACAT GCACGAATGC AATCAGACGG AGTGCGTGAC CAGTGCAGAN
0301 CCACGGCAGG GNAAACATTC ACTTCTGCTG CTGCAAGGGA TCGCGGTGCA ATTCCAACCA
0361 GAAATATATT AAAAGCACCA CGGAGGCAAC CACACAAGTG CCCAAGGAGA AGACGCAGGA
0421 CGGCAGCAAT TTGATATACA TCTACATTGG CACCTCCGTT TTCAGCGTGC TCATGGTCAT
0481 TGTTGGCATG GGCCTTCTTC T |
| in silico PCR Fragment |
0001 GATCTGCTTT ATCTAACGGC GCAGCTAACG CTGGTCTGCT GTCTGATTGG AATCCATGGA
0061 TCTATTTTGC CCGGAAGTCA TGGGATCATA GAATGCGAGC ACTTCGACGA GAAGATGTGC
0121 AACACAACGC AGCAATGTGA AACACGGATA GAGCACTGTA AGATGGAGGC GGATAAGTTT
0181 CCCAGCTGCT ATGTCCTTTG GTCGGTCAAC GAGACAACGG GCATCCTGCG CATCAAGATG
0241 AAGGGCTGCT TCACGGACAT GCACGAATGC AATCAGACGG AGTGCGTGAC CAGTGCAGAG
0301 CCACGGCAGG G-AAACATTC ACTTCTGCTG CTGCAAGGGA TCGCGGTGCA ATTCCAACCA
0361 GAAATATATT AAAAGCACCA CGGAGGCAAC CACACAAGTG CCCAAGGAGA AGACGCAGGA
0421 CGGCAGCAAT TTGATATACA TCTACATTGG CACCTCCGTT TTCAGCGTGC TCATGGTCAT
0481 TGTTGGCATG GGCCTTCTTC T |
| Assemble Data |
7904R-2.IR_full 1 GATCTGCTTTATCTAACGGCGCAGCNAACGCTGGTCTGCTGTCTGATTGGAATCCATGGA 60
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
7904R-2.in silico 1 GATCTGCTTTATCTAACGGCGCAGCTAACGCTGGTCTGCTGTCTGATTGGAATCCATGGA 60
7904R-2.IR_full 61 TCTATTTTGCCCGGAAGTCATGGGATCATAGAATGCGAGCACTTCGACGAGAAGATGTGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 61 TCTATTTTGCCCGGAAGTCATGGGATCATAGAATGCGAGCACTTCGACGAGAAGATGTGC 120
7904R-2.IR_full 121 AACACAACGCAGCAATGTGAAACACGGATAGAGCACTGTAAGATGGAGGCGGATAAGTTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 121 AACACAACGCAGCAATGTGAAACACGGATAGAGCACTGTAAGATGGAGGCGGATAAGTTT 180
7904R-2.IR_full 181 CCCAGCTGCTATGTCCTTTGGTCGGTCAACGAGACAACGGGCATCCTGCGCATCAAGATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 181 CCCAGCTGCTATGTCCTTTGGTCGGTCAACGAGACAACGGGCATCCTGCGCATCAAGATG 240
7904R-2.IR_full 241 AAGGGCTGCTTCACGGACATGCACGAATGCAATCAGACGGAGTGCGTGACCAGTGCAGAN 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 241 AAGGGCTGCTTCACGGACATGCACGAATGCAATCAGACGGAGTGCGTGACCAGTGCAGAG 300
7904R-2.IR_full 301 CCACGGCAGGGNAAACATTCACTTCTGCTGCTGCAAGGGATCGCGGTGCAATTCCAACCA 360
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 301 CCACGGCAGGG-AAACATTCACTTCTGCTGCTGCAAGGGATCGCGGTGCAATTCCAACCA 360
7904R-2.IR_full 361 GAAATATATTAAAAGCACCACGGAGGCAACCACACAAGTGCCCAAGGAGAAGACGCAGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 361 GAAATATATTAAAAGCACCACGGAGGCAACCACACAAGTGCCCAAGGAGAAGACGCAGGA 420
7904R-2.IR_full 421 CGGCAGCAATTTGATATACATCTACATTGGCACCTCCGTTTTCAGCGTGCTCATGGTCAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7904R-2.in silico 421 CGGCAGCAATTTGATATACATCTACATTGGCACCTCCGTTTTCAGCGTGCTCATGGTCAT 480
7904R-2.IR_full 481 TGTTGGCATGGGCCTTCTTCT 501
|||||||||||||||||||||
7904R-2.in silico 481 TGTTGGCATGGGCCTTCTTCT 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_169591.1 |
CG7904-RA (put), mRNA |
| 0.62 |
3 |
3 |
3 |
37 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0.2 |
1 |
6 |
3 |
7 |
NM_165676.1 |
CG11804-RB, transcript variant B (ced-6), mRNA |
| 0.2 |
1 |
6 |
3 |
7 |
NM_136644.2 |
CG11804-RC, transcript variant C (ced-6), mRNA |
| 0.2 |
1 |
6 |
3 |
7 |
NM_165675.1 |
CG11804-RA, transcript variant A (ced-6), mRNA |
| 0 |
0 |
3 |
5 |
5 |
NM_169203.1 |
CG11094-RB, transcript variant B (dsx), mRNA |
| 0 |
0 |
3 |
5 |
5 |
NM_079548.4 |
CG11094-RC, transcript variant C (dsx), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_135275.2 |
CG5958-RA (CG5958), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_143459.1 |
CG1964-RA (Kul), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079044.1 |
CG17876-RA (Amy-d), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_080421.3 |
CG18730-RA (Amy-p), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132267.4 |
CG12109-RB (Caf1-180), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_079939.2 |
CG5580-RA, transcript variant A (sbb), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206161.1 |
CG5580-RB, transcript variant B (sbb), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206162.1 |
CG5580-RC, transcript variant C (sbb), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136457.2 |
CG1358-RA, transcript variant A (CG1358), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165545.1 |
CG1358-RB, transcript variant B (CG1358), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165546.1 |
CG1358-RC, transcript variant C (CG1358), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_078501.2 |
CG5905-RA, transcript variant A (Nep1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_167059.1 |
CG5905-RB, transcript variant B (Nep1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057842.2 |
CG16858-RA (vkg), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141045.1 |
CG7632-RA (CG7632), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_170168.1 |
CG6863-RB, transcript variant B (tok), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_057531.3 |
CG6863-RA, transcript variant A (tok), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206758.1 |
CG9177-RC, transcript variant C (eIF5), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132870.3 |
CG9177-RB, transcript variant B (eIF5), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206757.1 |
CG9177-RD, transcript variant D (eIF5), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206754.1 |
CG9177-RG, transcript variant G (eIF5), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_206756.1 |
CG9177-RE, transcript variant E (eIF5), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167477.1 |
CG9177-RA, transcript variant A (eIF5), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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