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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7890R-1 |
| Symbol |
Hs3st-B |
Full Name |
Heparan sulfate 3-O sulfotransferase-B |
| CG No |
CG7890 |
Old CG No |
CG7890 |
| Synonyms |
Hs3st-B, CG7890, dHs3st-B |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
Goda E, Kamiyama S, Uno T, Yoshida H, Ueyama M, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S.
Identification and characterization of a novel Drosophila 3'-phosphoadenosine 5'-phosphosulfate transporter.
J Biol Chem (2006) 281(39) 28508-17
[
PubMed ID = 16873373
]
[
RRC reference
]
Ueyama M, Takemae H, Ohmae Y, Yoshida H, Toyoda H, Ueda R, Nishihara S.
Functional analysis of proteoglycan galactosyltransferase II RNA interference mutant flies.
J Biol Chem (2008) 283(10) 6076-84
[
PubMed ID = 18165227
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCACCATGGAGAAGACGCCC |
| Primer Seq. 3' |
aatctagaggtaccGGATCTATGTGCGGATGGTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
|
| Assemble Data |
7890R-1.IR full 1 GATCACCATGGAGAAGACGCCCAGCTACTTCGTCACCAAGGAGGTGCCGCAGCGCGTCTA 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 1 -ATCACCATGGAGAAGACGCCCAGCTACTTCGTCACCAAGGAGGTGCCGCAGCGCGTCTA 60
7890R-1.IR full 61 CCACATGAATCCGGCAACAAAATTACTGATTGTGGTGCGGGATCCGGTGACGCGGGCCAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 61 CCACATGAATCCGGCAACAAAATTACTGATTGTGGTGCGGGATCCGGTGACGCGGGCCAT 120
7890R-1.IR full 121 CTCCGACTACACACAGGCGGCGAGCAAGAAGGCGGACATGAAGCTCTTCGAGCAGCTGGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 121 CTCCGACTACACACAGGCGGCGAGCAAGAAGGCGGACATGAAGCTCTTCGAGCAGCTGGC 180
7890R-1.IR full 181 CTTCGTCAACGGTAGCTACTCGGTGGTGGACACCAACTGGGGCCCGGTGAAGATCGGCGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 181 CTTCGTCAACGGTAGCTACTCGGTGGTGGACACCAACTGGGGCCCGGTGAAGATCGGCGT 240
7890R-1.IR full 241 CTATGCACGCTATCTGGAGCGCTGGCTGCTCTACTTTCCGCTCTCGCAGCTGCTCTTCAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 241 CTATGCACGCTATCTGGAGCGCTGGCTGCTCTACTTTCCGCTCTCGCAGCTGCTCTTCAT 300
7890R-1.IR full 301 CAGCGGCGAGCGNCTGATCATGGATCCCGCCTACGAGATTGGACGCGTACAGGACTTCCT 360
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
7890R-1.in silico 301 CAGCGGCGAGCGGCTGATCATGGATCCCGCCTACGAGATTGGACGCGTACAGGACTTCCT 360
7890R-1.IR full 361 CGGTCTGAAGCGCGTGGTCACCGAGAAGCACTTCTACTTCAATGCCACCAAGGGCTTCCC 420
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7890R-1.in silico 361 CGGTCTGAAGCGCGTGGTCACCGAGAAGCACTTCTACTTCAATGCCACCAAGGGCTTCCC 420
7890R-1.IR full 421 CTGCCTCTTCAAGTCGGAGGCACGCTCCACGCCCCACTGTCTGGGTAAGACCAAGNGGCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
7890R-1.in silico 421 CTGCCTCTTCAAGTCGGAGGCACGCTCCACGCCCCACTGTCTGGGTAAGACCAAGGGGCG 480
7890R-1.IR full 481 CAACCATCCGCACATAGATCC 501
|||||||||||||||||||||
7890R-1.in silico 481 CAACCATCCGCACATAGATCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_133142.1 |
CG7890-RA (CG7890), mRNA |
| 0.62 |
3 |
8 |
3 |
7 |
NM_168288.1 |
CG32029-RA (CG32029), mRNA |
| 0.2 |
1 |
3 |
6 |
7 |
NM_176325.1 |
Ral guanine nucleotide exchange factor 2 CG8865-RB, transcript variant B (Rgl), mRNA |
| 0.2 |
1 |
3 |
6 |
7 |
NM_176327.1 |
Ral guanine nucleotide exchange factor 2 CG8865-RD, transcript variant D (Rgl), mRNA |
| 0.2 |
1 |
3 |
6 |
7 |
NM_176326.1 |
Ral guanine nucleotide exchange factor 2 CG8865-RC, transcript variant C (Rgl), mRNA |
| 0.2 |
1 |
3 |
3 |
3 |
NM_079958.2 |
Ral guanine nucleotide exchange factor 2 CG8865-RA, transcript variant A (Rgl), mRNA |
| 0 |
0 |
4 |
4 |
4 |
NM_167244.1 |
Transport and Golgi organization 5 CG32675-RB, transcript variant B (Tango5), mRNA |
| 0 |
0 |
4 |
4 |
4 |
NM_167242.1 |
Transport and Golgi organization 5 CG32675-RA, transcript variant A (Tango5), mRNA |
| 0 |
0 |
4 |
4 |
4 |
NM_167243.1 |
Transport and Golgi organization 5 CG32675-RC, transcript variant C (Tango5), mRNA |
| 0 |
0 |
3 |
6 |
7 |
NM_166535.1 |
plexus CG4444-RA (px), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_080048.2 |
Lobe CG10109-RA (L), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167085.1 |
CG3168-RB, transcript variant B (CG3168), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132117.1 |
CG3168-RA, transcript variant A (CG3168), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167086.1 |
CG3168-RC, transcript variant C (CG3168), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141591.1 |
CG11966-RA (CG11966), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079427.2 |
UDP-glucose-glycoprotein glucosyltransferase CG6850-RA (Ugt), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132991.1 |
CG12432-RA (CG12432), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136527.1 |
CG14755-RA (CG14755), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_137051.1 |
CG13337-RA (CG13337), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_137621.2 |
CG10444-RA (CG10444), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137992.1 |
CG5549-RA (CG5549), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168367.1 |
CG32046-RA, transcript variant A (CG32046), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168366.2 |
CG32046-RB, transcript variant B (CG32046), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043104.1 |
ion transport peptide CG13586-RB, transcript variant B (itp), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_138087.3 |
ion transport peptide CG13586-RA, transcript variant A (itp), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_135409.1 |
CG9468-RA (CG9468), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143096.2 |
CG11851-RA (CG11851), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142088.2 |
CG9631-RA (CG9631), mRNA |
| 0 |
0 |
0 |
17 |
31 |
NM_176229.1 |
CG33147-RA (CG33147), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_143262.1 |
CG14254-RA (CG14254), mRNA |
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