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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7846R-1 |
| Symbol |
Arp8 |
Full Name |
Actin-related protein 8 |
| CG No |
CG7846 |
Old CG No |
CG7846 |
| Synonyms |
Arp8, CG7846, dArp8 |
| Accession No (Link to NCBI) |
NM_133023.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCAACGATCCCGAGCCAGC |
| Primer Seq. 3' |
aatctagaggtaccAGTCGCAGGATACGGTCGTCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGCAACGAT CCCGAGCCAG CAGCACCAGC AGTGGCCGGC ATCCGgcGCC GcCNCCAATT
0061 GGGCTGCCCC AGcCGCAggc GCAGCCACTG GAGGCGCCAA AGATTATCGT CATCCATCCT
0121 GGATCGCAGC ACTTGAGGAT CGGCAGGGCG GCCGACCTTA ATCCGCTGAC CCTGCTGCAC
0181 GCGGTGGCCN TACAGGAGGC GTCCGGGGGC CAGCGATAGG CCGCNATCAT GATCCGCTGC
0241 TGCCGCCGCT GGACAACGTC AACTCAAACT CCGGCCTGAT GGTGGAGTTC GAGGAGCAGC
0301 GCCTGGTGGT GTCGCGCATT CTGCAGCACT GCGTCGTCGA TAACAAAAAC CGGTTGCGGG
0361 TGGCCACTCC GCCGCAGCAA CTGGCCCACT TCAACCGGAG CAGTCAGGCG GAGAAGGTGC
0421 CGGCGCCCAG TgGCCAGATG GCGGACGAGC CCTGGCTGGA TCGCGAGGCA CCCGTGCTGT
0481 TCGACGACCG TATCCTGCGA NTGGTACCTC T |
| in silico PCR Fragment |
0001 ATGCAACGAT CCCGAGCCAG CAGCACCAGC AGTGGCCGGC ATCCGGCGCC GCCGCCAATT
0061 GGGCTGCCCC AGCCGCAGGC GCAGCCACTG GAGGCGCCAA AGATTATCGT CATCCATCCT
0121 GGATCGCAGC ACTTGAGGAT CGGCAGGGCG GCCGACCTTA ATCCGCTGAC CCTGCTGCAC
0181 GCGGTGGCC- TACAGGAGGC GTCCGGGGGC CAGCGATAGG CCGC-ATCAT GATCCGCTGC
0241 TGCCGCCGCT GGACAACGTC AACTCAAACT CCGGCCTGAT GGTGGAGTTC GAGGAGCAGC
0301 GCCTGGTGGT GTCGCGCATT CTGCAGCACT GCGTCGTCGA TAACAAAAAC CGGTTGCGGG
0361 TGGCCACTCC GCCGCAGCAA CTGGCCCACT TCAACCGGAG CAGTCAGGCG GAGAAGGTGC
0421 CGGCGCCCAG TGGCCAGATG GCGGACGAGC CCTGGCTGGA TCGCGAGGCA CCCGTGCTGT
0481 TCGACGACCG TATCCTGCGA ---------C T |
| Assemble Data |
7846R-1.IR_full 1 ATGCAACGATCCCGAGCCAGCAGCACCAGCAGTGGCCGGCATCCGGCGCCGCCNCCAATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
7846R-1.in silico 1 ATGCAACGATCCCGAGCCAGCAGCACCAGCAGTGGCCGGCATCCGGCGCCGCCGCCAATT 60
7846R-1.IR_full 61 GGGCTGCCCCAGCCGCAGGCGCAGCCACTGGAGGCGCCAAAGATTATCGTCATCCATCCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 61 GGGCTGCCCCAGCCGCAGGCGCAGCCACTGGAGGCGCCAAAGATTATCGTCATCCATCCT 120
7846R-1.IR_full 121 GGATCGCAGCACTTGAGGATCGGCAGGGCGGCCGACCTTAATCCGCTGACCCTGCTGCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 121 GGATCGCAGCACTTGAGGATCGGCAGGGCGGCCGACCTTAATCCGCTGACCCTGCTGCAC 180
7846R-1.IR_full 181 GCGGTGGCCNTACAGGAGGCGTCCGGGGGCCAGCGATAGGCCGCNATCATGATCCGCTGC 240
||||||||| |||||||||||||||||||||||||||||||||| |||||||||||||||
7846R-1.in silico 181 GCGGTGGCC-TACAGGAGGCGTCCGGGGGCCAGCGATAGGCCGC-ATCATGATCCGCTGC 240
7846R-1.IR_full 241 TGCCGCCGCTGGACAACGTCAACTCAAACTCCGGCCTGATGGTGGAGTTCGAGGAGCAGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 241 TGCCGCCGCTGGACAACGTCAACTCAAACTCCGGCCTGATGGTGGAGTTCGAGGAGCAGC 300
7846R-1.IR_full 301 GCCTGGTGGTGTCGCGCATTCTGCAGCACTGCGTCGTCGATAACAAAAACCGGTTGCGGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 301 GCCTGGTGGTGTCGCGCATTCTGCAGCACTGCGTCGTCGATAACAAAAACCGGTTGCGGG 360
7846R-1.IR_full 361 TGGCCACTCCGCCGCAGCAACTGGCCCACTTCAACCGGAGCAGTCAGGCGGAGAAGGTGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 361 TGGCCACTCCGCCGCAGCAACTGGCCCACTTCAACCGGAGCAGTCAGGCGGAGAAGGTGC 420
7846R-1.IR_full 421 CGGCGCCCAGTGGCCAGATGGCGGACGAGCCCTGGCTGGATCGCGAGGCACCCGTGCTGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7846R-1.in silico 421 CGGCGCCCAGTGGCCAGATGGCGGACGAGCCCTGGCTGGATCGCGAGGCACCCGTGCTGT 480
7846R-1.IR_full 481 TCGACGACCGTATCCTGCGANTGGTACCTCT 511
|||||||||||||||||||| ||
7846R-1.in silico 481 TCGACGACCGTATCCTGCGA---------CT 511
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_133023.2 |
CG7846-RA (CG7846), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_170294.1 |
CG6348-RB, transcript variant B (ro), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_079797.2 |
CG6348-RA, transcript variant A (ro), mRNA |
| 0 |
0 |
3 |
8 |
10 |
NM_167340.1 |
CG32648-RA (Pde9), mRNA |
| 0 |
0 |
3 |
6 |
20 |
NM_132004.2 |
CG4136-RA (CG4136), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_079416.2 |
CG4166-RA (not), mRNA |
| 0 |
0 |
2 |
10 |
8 |
NM_078545.2 |
CG12653-RA (btd), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_132650.1 |
CG10617-RA (CG10617), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_167218.1 |
CG32685-RC (CG32685), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_078946.3 |
CG2328-RA (eve), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_134555.1 |
CG15454-RA (CG15454), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_140423.2 |
CG8783-RA, transcript variant A (CG8783), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_168570.1 |
CG8783-RB, transcript variant B (CG8783), mRNA |
| 0 |
0 |
2 |
3 |
24 |
NM_057326.4 |
CG12212-RA, transcript variant A (peb), mRNA |
| 0 |
0 |
2 |
3 |
24 |
NM_001014722.1 |
CG12212-RB, transcript variant B (peb), mRNA |
| 0 |
0 |
2 |
3 |
19 |
NM_167487.1 |
CG32577-RA (disco-r), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001043277.1 |
CG31163-RD, transcript variant D (CG31163), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_143645.1 |
CG1976-RA (RhoGAP100F), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137594.2 |
CG11018-RA (CG11018), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_137729.1 |
CG9752-RA (CG9752), mRNA |
| 0 |
0 |
2 |
2 |
20 |
NM_080026.2 |
CG10847-RB, transcript variant B (enc), mRNA |
| 0 |
0 |
2 |
2 |
20 |
NM_206270.1 |
CG10847-RA, transcript variant A (enc), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_167686.1 |
CG11940-RB, transcript variant B (CG11940), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_001031910.1 |
CG11566-RA (CG11566), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_134519.1 |
CG11940-RA, transcript variant A (CG11940), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_001031911.1 |
CG33670-RA (CG33670), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_001031888.1 |
CG33691-RB, transcript variant B (CG33691), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_001031885.1 |
CG33692-RB, transcript variant B (CG33692), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_001031887.1 |
CG33691-RC, transcript variant C (CG33691), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_001031889.1 |
CG33691-RA, transcript variant A (CG33691), mRNA |
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