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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7845R-2 
 Symbol CG7845  Full Name CG7845 
 CG No CG7845  Old CG No CG7845 
 Synonyms CG7845 
 Accession No (Link to NCBI) NM_136356.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAACTACACGGCGAAGCACGAA 
 Primer Seq. 3' 
aatctagaggtaccATCTGCTTACCGTCGGCATTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AACTACACGG CGAAGCNCGA ACTGTATGTG GGAACACACA CGGGCTCCTT TAAACACCTG 
0061 GTGCCTGCAT CCGAGAAGAC CCCTTATGGA CAAAGCAACC TGAGTGATCT GTGCGCCCTG 
0121 GATAAGGATT CCCGCGTCAC ATCTCTGGCC TTTGGCAACG ACGATCAGAC GGAGATCCTT 
0181 CTAGGCCGGG CCAAGGACGC CGTGGAGATT CACTCCTTCA CAGAAAAGGG ACATTCTAGG 
0241 AACACAGTAT CCTTCAATGC TGCGCCAATT GTTGGTCTGG CCCGATACAA TGACAAGCTT 
0301 ATTGCCGGCA TTGGCAACGG AGAGATACAG AGCCTGGAGC TAGAGGCGAA CGACGAGTCC 
0361 GAGCCCGTGG TTATCGCCAC GGGTGACCAA ATGGACCACC TGCGCCAGTG CGCCCAAGTC 
0421 AAAAGCATTG TAGCAACTGG CGGCAAAGAG CGTCAAAACA ATCTAAAGGT CTACGATTTA 
0481 AATGCCGACG GTAAGCAGAT  
 in silico PCR Fragment 
0001 AACTACACGG CGAAGCACGA ACTGTATGTG GGAACACACA CGGGCTCCTT TAAACACCTG 
0061 GTGCCTGCAT CCGAGAAGAC CCCTTATGGA CAAAGCAACC TGAGTGATCT GTGCGCCCTG 
0121 GATAAGGATT CCCGCGTCAC ATCTCTGGCC TTTGGCAACG ACGATCAGAC GGAGATCCTT 
0181 CTAGGCCGGG CCAAGGACGC CGTGGAGATT CACTCCTTCA CAGAAAAGGG ACATTCTAGG 
0241 AACACAGTAT CCTTCAATGC TGCGCCAATT GTTGGTCTGG CCCGATACAA TGACAAGCTT 
0301 ATTGCCGGCA TTGGCAACGG AGAGATACAG AGCCTGGAGC TAGAGGCGAA CGACGAGTCC 
0361 GAGCCCGTGG TTATCGCCAC GGGTGACCAA ATGGACCACC TGCGCCAGTG CGCCCAAGTC 
0421 AAAAGCATTG TAGCAACTGG CGGCAAAGAG CGTCAAAACA ATCTAAAGGT CTACGATTTA 
0481 AATGCCGACG GTAAGCAGAT  
 Assemble Data 

7845R-2.IR_full       1   AACTACACGGCGAAGCNCGAACTGTATGTGGGAACACACACGGGCTCCTTTAAACACCTG 60
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     1   AACTACACGGCGAAGCACGAACTGTATGTGGGAACACACACGGGCTCCTTTAAACACCTG 60


7845R-2.IR_full       61  GTGCCTGCATCCGAGAAGACCCCTTATGGACAAAGCAACCTGAGTGATCTGTGCGCCCTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     61  GTGCCTGCATCCGAGAAGACCCCTTATGGACAAAGCAACCTGAGTGATCTGTGCGCCCTG 120


7845R-2.IR_full       121 GATAAGGATTCCCGCGTCACATCTCTGGCCTTTGGCAACGACGATCAGACGGAGATCCTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     121 GATAAGGATTCCCGCGTCACATCTCTGGCCTTTGGCAACGACGATCAGACGGAGATCCTT 180


7845R-2.IR_full       181 CTAGGCCGGGCCAAGGACGCCGTGGAGATTCACTCCTTCACAGAAAAGGGACATTCTAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     181 CTAGGCCGGGCCAAGGACGCCGTGGAGATTCACTCCTTCACAGAAAAGGGACATTCTAGG 240


7845R-2.IR_full       241 AACACAGTATCCTTCAATGCTGCGCCAATTGTTGGTCTGGCCCGATACAATGACAAGCTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     241 AACACAGTATCCTTCAATGCTGCGCCAATTGTTGGTCTGGCCCGATACAATGACAAGCTT 300


7845R-2.IR_full       301 ATTGCCGGCATTGGCAACGGAGAGATACAGAGCCTGGAGCTAGAGGCGAACGACGAGTCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     301 ATTGCCGGCATTGGCAACGGAGAGATACAGAGCCTGGAGCTAGAGGCGAACGACGAGTCC 360


7845R-2.IR_full       361 GAGCCCGTGGTTATCGCCACGGGTGACCAAATGGACCACCTGCGCCAGTGCGCCCAAGTC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     361 GAGCCCGTGGTTATCGCCACGGGTGACCAAATGGACCACCTGCGCCAGTGCGCCCAAGTC 420


7845R-2.IR_full       421 AAAAGCATTGTAGCAACTGGCGGCAAAGAGCGTCAAAACAATCTAAAGGTCTACGATTTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7845R-2.in silico     421 AAAAGCATTGTAGCAACTGGCGGCAAAGAGCGTCAAAACAATCTAAAGGTCTACGATTTA 480


7845R-2.IR_full       481 AATGCCGACGGTAAGCAGAT 500
                          ||||||||||||||||||||
7845R-2.in silico     481 AATGCCGACGGTAAGCAGAT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136356.2  CG7845-RA (CG7845), mRNA 
0.2   1  2  3  5  NM_170085.2  CG31414-RA (CG31414), mRNA 
0   0  2  5  2  NM_141360.1  CG1077-RA (CG1077), mRNA 
0   0  2  4  4  NM_136725.3  CG12910-RA (CG12910), mRNA 
0   0  1  3  4  NM_165703.1  CG1519-RA, transcript variant A (Prosalpha7), mRNA 
0   0  1  3  4  NM_078944.4  CG1519-RB, transcript variant B (Prosalpha7), mRNA 
0   0  1  3  3  NM_079840.2  CG7938-RA (Sry-beta), mRNA 
0   0  1  3  2  NM_132802.2  CG9201-RB, transcript variant B (Grip128), mRNA 
0   0  1  3  2  NM_001038757.1  CG9201-RC, transcript variant C (Grip128), mRNA 
0   0  1  2  4  NM_141881.1  CG18547-RA (CG18547), mRNA 
0   0  1  2  4  NM_136774.1  CG12325-RA (CG12325), mRNA 
0   0  1  2  3  NM_137901.1  CG9896-RA (CG9896), mRNA 
0   0  0  5  3  NM_165287.1  CG15825-RA, transcript variant A (CG15825), mRNA 
0   0  0  5  3  NM_136115.2  CG15825-RB, transcript variant B (CG15825), mRNA 
0   0  0  5  2  NM_138144.2  CG2765-RA (CG2765), mRNA 
0   0  0  4  3  NM_165638.1  CG8229-RA, transcript variant A (CG8229), mRNA 
0   0  0  4  3  NM_136588.2  CG8229-RC, transcript variant C (CG8229), mRNA 
0   0  0  4  3  NM_136634.2  CG1975-RA (Rep2), mRNA 
0   0  0  4  3  NM_057425.2  CG9450-RA (tud), mRNA 
0   0  0  4  2  NM_166262.1  CG5756-RB, transcript variant B (CG5756), mRNA 
0   0  0  4  2  NM_141067.2  CG7186-RA (SAK), mRNA 
0   0  0  4  2  NM_137448.2  CG5756-RA, transcript variant A (CG5756), mRNA 
0   0  0  3  9  NM_168023.1  CG11526-RA, transcript variant A (CG11526), mRNA 
0   0  0  3  9  NM_139549.1  CG11526-RB, transcript variant B (CG11526), mRNA 
0   0  0  3  6  NM_167218.1  CG32685-RC (CG32685), mRNA 
0   0  0  3  5  NM_001031924.1  CG9130-RB, transcript variant B (CG9130), mRNA 
0   0  0  3  5  NM_001031925.1  CG9130-RA, transcript variant A (CG9130), mRNA 
0   0  0  3  5  NM_001031921.1  CG9133-RD, transcript variant D (CG9133), mRNA 
0   0  0  3  5  NM_001031923.1  CG9133-RC, transcript variant C (CG9133), mRNA 
0   0  0  3  5  NM_001031922.1  CG9133-RB, transcript variant B (CG9133), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
++ 
DB
++ 
DC
++ 
DM
+ 
VM
+ 
VB
+- 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
+ 
VB
++ 
Dcompartment   Penetrance
++     

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