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NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7843R-4 
 Symbol CG7843  Full Name CG7843 
 CG No CG7843  Old CG No CG7843 
 Synonyms anon-WO0118547.166, CG7843 
 Accession No (Link to NCBI) NM_136359.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGGTGATTCCGACGATGAG 
 Primer Seq. 3' 
aatctagaggtaccTCGTCCTGGGTGTCGAGAAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGGTGATT CCGACGATGA GTACGACCGC AAGCGACGGG ACAAATTCCG CGGGGAGCGC 
0061 GAGAGTTACC GTACGGAACG CCGGGATGAC CGCCGGCCGG TGGGcGGCTC TNGCCGGTGC 
0121 CCGCGACGAG TgGGCGGAGC GCAATCCGTT CAGGGGCGGA GCTTCCGCAG GCGGAGGAGG 
0181 AGCTCGCCAC AGACCAGACT ACAGCGACTA CCGTGGCCCG GGAGCTAGAC CNACGCTATG 
0241 GGTCACCCGT CCGTGATTTG CCCCCAGCCA AGCGTATGCG CCCGGACTGG GGGGACGGCG 
0301 ATGTGCGCGC CAATCCCCGC TTTGGGGGCT ATGATCCGTA TTTGATGCAG GCCTGGAACG 
0361 ACCACTACCA GTCGATGCAT TCGGCCTACT CCCCACGGTG GCCATGCACC ACCTGTTTNC 
0421 GGGAGTCCAT AGGCGGCGGC GGAGGCGACA CCCTTACCCA ACCAGCCATG CTCAACCTGA 
0481 AGCAGTTTCT CGACACCCAG GACGA 
 in silico PCR Fragment 
0001 ATGGGTGATT CCGACGATGA GTACGACCGC AAGCGACGGG ACAAATTCCG CGGGGAGCGC 
0061 GAGAGTTACC GTACGGAACG CCGGGATGAC CGCCGGCCGG TGGGCGGCTC T-GCCGGTGC 
0121 CCGCGACGAG TGGGCGGAGC GCAATCCGTT CAGGGGCGGA GCTTCCGCAG GCGGAGGAGG 
0181 AGCTCGCCAC AGACCAGACT ACAGCGACTA CCGTGGCCCG GGAGCTAGAC C-ACGCTATG 
0241 GGTCACCCGT CCGTGATTTG CCCCCAGCCA AGCGTATGCG CCCGGACTGG GGGGACGGCG 
0301 ATGTGCGCGC CAATCCCCGC TTTGGGGGCT ATGATCCGTA TTTGATGCAG GCCTGGAACG 
0361 ACCACTACCA GTCGATGCAT TCGGCCTACT -CCCACGGTG GCCATGCACC ACCTGTT--C 
0421 GGGAGTCCAT AGGCGGCGGC GGAGGCGACA CCCTTACCCA ACCAGCCATG CTCAACCTGA 
0481 AGCAGTTTCT CGACACCCAG GACGA 
 Assemble Data 

7843R-4.IR_full       1   ATGGGTGATTCCGACGATGAGTACGACCGCAAGCGACGGGACAAATTCCGCGGGGAGCGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7843R-4.in silico     1   ATGGGTGATTCCGACGATGAGTACGACCGCAAGCGACGGGACAAATTCCGCGGGGAGCGC 60


7843R-4.IR_full       61  GAGAGTTACCGTACGGAACGCCGGGATGACCGCCGGCCGGTGGGCGGCTCTNGCCGGTGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
7843R-4.in silico     61  GAGAGTTACCGTACGGAACGCCGGGATGACCGCCGGCCGGTGGGCGGCTCT-GCCGGTGC 120


7843R-4.IR_full       121 CCGCGACGAGTGGGCGGAGCGCAATCCGTTCAGGGGCGGAGCTTCCGCAGGCGGAGGAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7843R-4.in silico     121 CCGCGACGAGTGGGCGGAGCGCAATCCGTTCAGGGGCGGAGCTTCCGCAGGCGGAGGAGG 180


7843R-4.IR_full       181 AGCTCGCCACAGACCAGACTACAGCGACTACCGTGGCCCGGGAGCTAGACCNACGCTATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
7843R-4.in silico     181 AGCTCGCCACAGACCAGACTACAGCGACTACCGTGGCCCGGGAGCTAGACC-ACGCTATG 240


7843R-4.IR_full       241 GGTCACCCGTCCGTGATTTGCCCCCAGCCAAGCGTATGCGCCCGGACTGGGGGGACGGCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7843R-4.in silico     241 GGTCACCCGTCCGTGATTTGCCCCCAGCCAAGCGTATGCGCCCGGACTGGGGGGACGGCG 300


7843R-4.IR_full       301 ATGTGCGCGCCAATCCCCGCTTTGGGGGCTATGATCCGTATTTGATGCAGGCCTGGAACG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7843R-4.in silico     301 ATGTGCGCGCCAATCCCCGCTTTGGGGGCTATGATCCGTATTTGATGCAGGCCTGGAACG 360


7843R-4.IR_full       361 ACCACTACCAGTCGATGCATTCGGCCTACTCCCCACGGTGGCCATGCACCACCTGTTTNC 420
                          |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||  |
7843R-4.in silico     361 ACCACTACCAGTCGATGCATTCGGCCTACT-CCCACGGTGGCCATGCACCACCTGTT--C 420


7843R-4.IR_full       421 GGGAGTCCATAGGCGGCGGCGGAGGCGACACCCTTACCCAACCAGCCATGCTCAACCTGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7843R-4.in silico     421 GGGAGTCCATAGGCGGCGGCGGAGGCGACACCCTTACCCAACCAGCCATGCTCAACCTGA 480


7843R-4.IR_full       481 AGCAGTTTCTCGACACCCAGGACGA 505
                          |||||||||||||||||||||||||
7843R-4.in silico     481 AGCAGTTTCTCGACACCCAGGACGA 505



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_136359.2  CG7843-RA, transcript variant A (CG7843), mRNA 
100   482  0  0  4  NM_165459.1  CG7843-RD, transcript variant D (CG7843), mRNA 
100   482  0  0  4  NM_165458.1  CG7843-RC, transcript variant C (CG7843), mRNA 
93.98   453  2  2  6  NM_165460.1  CG7843-RB, transcript variant B (CG7843), mRNA 
0.2   1  2  4  4  NM_078845.3  CG15274-RA (GABA-B-R1), mRNA 
0   0  4  5  3  NM_140943.2  CG32227-RA (CG32227), mRNA 
0   0  4  4  2  NM_132086.1  CG15894-RA (CG15894), mRNA 
0   0  4  2  9  NM_134517.2  CG15618-RA (CG15618), mRNA 
0   0  4  2  8  NM_139462.1  CG13800-RA (CG13800), mRNA 
0   0  2  2  8  NM_078544.2  CG1689-RA (lz), mRNA 
0   0  2  2  4  NM_131990.1  CG12732-RA (CG12732), mRNA 
0   0  1  5  6  NM_167330.2  CG17762-RD, transcript variant D (tomosyn), mRNA 
0   0  1  5  6  NM_167329.2  CG17762-RC, transcript variant C (tomosyn), mRNA 
0   0  1  5  6  NM_167328.2  CG17762-RA, transcript variant A (tomosyn), mRNA 
0   0  1  5  6  NM_132591.3  CG17762-RB, transcript variant B (tomosyn), mRNA 
0   0  1  5  3  NM_140925.2  CG7323-RA (CG7323), mRNA 
0   0  1  3  6  NM_134527.1  CG15322-RA (CG15322), mRNA 
0   0  1  3  5  NM_079864.2  CG1470-RA (Gycbeta100B), mRNA 
0   0  1  3  4  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
0   0  1  3  3  NM_169948.1  CG17299-RC, transcript variant C (SNF4Agamma), mRNA 
0   0  1  3  3  NM_169949.1  CG17299-RE, transcript variant E (SNF4Agamma), mRNA 
0   0  1  3  3  NM_001043273.1  CG17299-RJ, transcript variant J (SNF4Agamma), mRNA 
0   0  1  3  3  NM_080509.2  CG17299-RB, transcript variant B (SNF4Agamma), mRNA 
0   0  1  3  3  NM_169946.1  CG17299-RA, transcript variant A (SNF4Agamma), mRNA 
0   0  1  3  3  NM_165827.1  CG13204-RB, transcript variant B (CG13204), mRNA 
0   0  1  3  3  NM_136834.1  CG13204-RA, transcript variant A (CG13204), mRNA 
0   0  1  3  3  NM_001043272.1  CG17299-RK, transcript variant K (SNF4Agamma), mRNA 
0   0  1  3  3  NM_001043271.1  CG17299-RL, transcript variant L (SNF4Agamma), mRNA 
0   0  1  3  3  NM_169950.1  CG17299-RG, transcript variant G (SNF4Agamma), mRNA 
0   0  1  3  3  NM_001043274.1  CG17299-RI, transcript variant I (SNF4Agamma), mRNA 

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