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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7832R-3 |
| Symbol |
l(3)L1231 |
Full Name |
lethal (3) L1231 |
| CG No |
CG7832 |
Old CG No |
CG7832 |
| Synonyms |
LD14744, BEST:LD14744, CG7832, l(3)L1231 |
| Accession No (Link to NCBI) |
NM_142122.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAGGATTGCCACAGCAGCAG |
| Primer Seq. 3' |
aatctagaggtaccCGAGGTGGCTATCTTCTCCAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAGGATTGC CACAGCAGCA GCAGCCGCAA CAGCAACAGC GATAGcCGCA GCAACAGCCG
0061 CAGCAGCAGC AGCaACGCTG ACGCCCGTGG AGAGCATTGC CGGCAAGACG ACGTCCGAGG
0121 ACTCGGATCC CTATGCCTTC ACCGAGACTG TGGCCGTCAC ACCACCCATT CTATTCAATG
0181 CACAGAAATC GAGAGCCCGC CTAACCGACA GCAATAGAGG CAGCAACAAG AGGCAGACGG
0241 CAGCAACAGC TGCGGCCAAC AGAAAGGCGA ACCTGGTGGC CCAACTGAGT GTCACAGAGG
0301 CAGCAAAGGC GCAGGCGTCT TTGGCAAGCA ACAACACAAC GAATTTCCAT CATGTCACGC
0361 AATCTCAGAG ACAGTCGACG GCGCTGCAGT TGCAATTGCC ACTGCAATCC CAGTCACAGT
0421 CGCAGGCCTC GcCGANAGCG GGCCACCAAC GTGTGCATAG TCCGCCCGCA GCAACAGCAG
0481 CTGGAGAAGA TAGCCACCTC G |
| in silico PCR Fragment |
0001 GAAGGATTGC CACAGCAGCA GCAGCCGCAA CAGCAACAGC GATAGCCGCA GCAACAGCCG
0061 CAGCAGCAGC AGCAACGCTG ACGCCCGTGG AGAGCATTGC CGGCAAGACG ACGTCCGAGG
0121 ACTCGGATCC CTATGCCTTC ACCGAGACTG TGGCCGTCAC ACCACCCATT CTATTCAATG
0181 CACAGAAATC GAGAGCCCGC CTAACCGACA GCAATAGAGG CAGCAACAAG AGGCAGACGG
0241 CAGCAACAGC TGCGGCCAAC AGAAAGGCGA ACCTGGTGGC CCAACTGAGT GTCACAGAGG
0301 CAGCAAAGGC GCAGGCGTCT TTGGCAAGCA ACAACACAAC GAATTTCCAT CATGTCACGC
0361 AATCTCAGAG ACAGTCGACG GCGCTGCAGT TGCAATTGCC ACTGCAATCC CAGTCACAGT
0421 CGCAGGCCTC GCCGA-AGCG GGCCACCAAC GTGTGCATAG TCCGCCCGCA GCAACAGCAG
0481 CTGGAGAAGA TAGCCACCTC G |
| Assemble Data |
7832R-3.IR_full 1 GAAGGATTGCCACAGCAGCAGCAGCCGCAACAGCAACAGCGATAGCCGCAGCAACAGCCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 1 GAAGGATTGCCACAGCAGCAGCAGCCGCAACAGCAACAGCGATAGCCGCAGCAACAGCCG 60
7832R-3.IR_full 61 CAGCAGCAGCAGCAACGCTGACGCCCGTGGAGAGCATTGCCGGCAAGACGACGTCCGAGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 61 CAGCAGCAGCAGCAACGCTGACGCCCGTGGAGAGCATTGCCGGCAAGACGACGTCCGAGG 120
7832R-3.IR_full 121 ACTCGGATCCCTATGCCTTCACCGAGACTGTGGCCGTCACACCACCCATTCTATTCAATG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 121 ACTCGGATCCCTATGCCTTCACCGAGACTGTGGCCGTCACACCACCCATTCTATTCAATG 180
7832R-3.IR_full 181 CACAGAAATCGAGAGCCCGCCTAACCGACAGCAATAGAGGCAGCAACAAGAGGCAGACGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 181 CACAGAAATCGAGAGCCCGCCTAACCGACAGCAATAGAGGCAGCAACAAGAGGCAGACGG 240
7832R-3.IR_full 241 CAGCAACAGCTGCGGCCAACAGAAAGGCGAACCTGGTGGCCCAACTGAGTGTCACAGAGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 241 CAGCAACAGCTGCGGCCAACAGAAAGGCGAACCTGGTGGCCCAACTGAGTGTCACAGAGG 300
7832R-3.IR_full 301 CAGCAAAGGCGCAGGCGTCTTTGGCAAGCAACAACACAACGAATTTCCATCATGTCACGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 301 CAGCAAAGGCGCAGGCGTCTTTGGCAAGCAACAACACAACGAATTTCCATCATGTCACGC 360
7832R-3.IR_full 361 AATCTCAGAGACAGTCGACGGCGCTGCAGTTGCAATTGCCACTGCAATCCCAGTCACAGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 361 AATCTCAGAGACAGTCGACGGCGCTGCAGTTGCAATTGCCACTGCAATCCCAGTCACAGT 420
7832R-3.IR_full 421 CGCAGGCCTCGCCGANAGCGGGCCACCAACGTGTGCATAGTCCGCCCGCAGCAACAGCAG 480
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
7832R-3.in silico 421 CGCAGGCCTCGCCGA-AGCGGGCCACCAACGTGTGCATAGTCCGCCCGCAGCAACAGCAG 480
7832R-3.IR_full 481 CTGGAGAAGATAGCCACCTCG 501
|||||||||||||||||||||
7832R-3.in silico 481 CTGGAGAAGATAGCCACCTCG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
3 |
26 |
139 |
NM_142122.2 |
CG7832-RA (CG7832), mRNA |
| 2.28 |
11 |
10 |
46 |
214 |
NM_080079.2 |
CG32120-RA (sens), mRNA |
| 2.07 |
10 |
38 |
233 |
604 |
NM_143575.2 |
CG12071-RA, transcript variant A (CG12071), mRNA |
| 2.07 |
10 |
38 |
233 |
604 |
NM_176592.1 |
CG12071-RB, transcript variant B (CG12071), mRNA |
| 1.65 |
8 |
20 |
109 |
332 |
NM_168096.1 |
CG32245-RB, transcript variant B (CG32245), mRNA |
| 1.65 |
8 |
13 |
51 |
265 |
NM_080047.2 |
CG8491-RA (kto), mRNA |
| 1.65 |
8 |
11 |
55 |
178 |
NM_139660.3 |
CG32245-RA, transcript variant A (CG32245), mRNA |
| 1.65 |
8 |
11 |
55 |
178 |
NM_168095.2 |
CG32245-RC, transcript variant C (CG32245), mRNA |
| 1.45 |
7 |
9 |
48 |
202 |
NM_143623.1 |
CG1804-RA (kek6), mRNA |
| 1.24 |
6 |
10 |
88 |
288 |
NM_001032408.1 |
CG33956-RD, transcript variant D (kay), mRNA |
| 1.24 |
6 |
8 |
35 |
118 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 1.24 |
6 |
5 |
48 |
177 |
NM_206382.1 |
CG5151-RB, transcript variant B (CG5151), mRNA |
| 1.24 |
6 |
4 |
37 |
121 |
NM_140587.1 |
CG5151-RA, transcript variant A (CG5151), mRNA |
| 1.03 |
5 |
20 |
174 |
589 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0.82 |
4 |
14 |
133 |
357 |
NM_001038766.1 |
CG32529-RD, transcript variant D (CG32529), mRNA |
| 0.82 |
4 |
14 |
111 |
260 |
NM_134516.2 |
CG32529-RA, transcript variant A (CG32529), mRNA |
| 0.82 |
4 |
14 |
103 |
224 |
NM_167688.1 |
CG32529-RC, transcript variant C (CG32529), mRNA |
| 0.82 |
4 |
2 |
12 |
73 |
NM_206713.1 |
CG14411-RB, transcript variant B (CG14411), mRNA |
| 0.82 |
4 |
2 |
12 |
73 |
NM_132744.1 |
CG14411-RA, transcript variant A (CG14411), mRNA |
| 0.62 |
3 |
58 |
248 |
773 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 0.62 |
3 |
58 |
248 |
773 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 0.62 |
3 |
58 |
248 |
770 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 0.62 |
3 |
56 |
267 |
619 |
NM_132246.2 |
CG10555-RA (CG10555), mRNA |
| 0.62 |
3 |
42 |
179 |
641 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 0.62 |
3 |
33 |
94 |
322 |
NM_079321.2 |
CG10571-RA (ara), mRNA |
| 0.62 |
3 |
32 |
98 |
338 |
NM_001014631.1 |
CG31243-RH, transcript variant H (cpo), mRNA |
| 0.62 |
3 |
32 |
98 |
336 |
NM_001014632.1 |
CG31243-RG, transcript variant G (cpo), mRNA |
| 0.62 |
3 |
13 |
54 |
213 |
NM_166973.1 |
CG12206-RB, transcript variant B (CG12206), mRNA |
| 0.62 |
3 |
13 |
52 |
199 |
NM_166974.1 |
CG12206-RC, transcript variant C (CG12206), mRNA |
| 0.62 |
3 |
13 |
52 |
199 |
NM_130704.1 |
CG12206-RA, transcript variant A (CG12206), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
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Merge
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Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
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