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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7758R-1 |
| Symbol |
ppl |
Full Name |
pumpless |
| CG No |
CG7758 |
Old CG No |
CG7758 |
| Synonyms |
CG7758, l(3)78Cb, l(3)00217, ppl |
| Accession No (Link to NCBI) |
NM_079473.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAATTCGCAAGGATTGGGCT |
| Primer Seq. 3' |
aatctagaggtaccGATTAGCTGCTGGTTCTAGTG |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 GAAATTCGCA AGGATTGGGC TGCAGGcCGC CCGCCAGCTT AGTGTCACGC CCCTTGGcGC
0061 CGTCCAGGCT CGCGCCATTC ACCTGACAAG CCTTCTAGCC AAAGAACGCC GATACACAAA
0121 CAAACACGAG TGGGTGGAGG TGGTATCCGG CANGCAATGC CATAGTAGGC ATCTNCCAGC
0181 TACGCCCAGG AGGCTCTCGG GGATGTGGTG TTCGCCCAAC TCCCAGAACC CGGCACGGAA
0241 CTCAAGCAGG ATGACGAATG TGGGGCCCTG GAGAGCGTTA AAGCGGCTAG CGAGGTGTAT
0301 TCACCCGTTA GTGGCAAGGT AATTGAAAAG AATGCCGAGG TGGAGGACAC ACCGGCCCTA
0361 GTCAACAGCA GTTGCTATGA GAAGGGCTGG CTTTTCAAGG TGGACCTGAA GAATCCCAAG
0421 GAACTCGAAG CCCTTATGAC CGAGGATCAG TACAAAGCCT TCCTGAGCAG CAGTGGGGAT
0481 CACTAGAACC AGCAGCTAAT C |
| in silico PCR Fragment |
0001 GAAATTCGCA AGGATTGGGC TGCAGGCCGC CCGCCAGCTT AGTGTCACGC CCCTTGGCGC
0061 CGTCCAGGCT CGCGCCATTC ACCTGACAAG CCTTCTAGCC AAAGAACGCC GATACACAAA
0121 CAAACACGAG TGGGTGGAGG TGGTATCCGG CA-GCAATGC CATAGTAGGC ATCT-CCAGC
0181 TACGCCCAGG AGGCTCTCGG GGATGTGGTG TTCGCCCAAC TCCCAGAACC CGGCACGGAA
0241 CTCAAGCAGG ATGACGAATG TGGGGCCCTG GAGAGCGTTA AAGCGGCTAG CGAGGTGTAT
0301 TCACCCGTTA GTGGCAAGGT AATTGAAAAG AATGCCGAGG TGGAGGACAC ACCGGCCCTA
0361 GTCAACAGCA GTTGCTATGA GAAGGGCTGG CTTTTCAAGG TGGACCTGAA GAATCCCAAG
0421 GAACTCGAAG CCCTTATGAC CGAGGATCAG TACAAAGCCT TCCTGAGCAG CAGTGGGGAT
0481 CACTAGAACC AGCAGCTAAT C |
| Assemble Data |
7758R-1.IR_full 1 GAAATTCGCAAGGATTGGGCTGCAGGCCGCCCGCCAGCTTAGTGTCACGCCCCTTGGCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 1 GAAATTCGCAAGGATTGGGCTGCAGGCCGCCCGCCAGCTTAGTGTCACGCCCCTTGGCGC 60
7758R-1.IR_full 61 CGTCCAGGCTCGCGCCATTCACCTGACAAGCCTTCTAGCCAAAGAACGCCGATACACAAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 61 CGTCCAGGCTCGCGCCATTCACCTGACAAGCCTTCTAGCCAAAGAACGCCGATACACAAA 120
7758R-1.IR_full 121 CAAACACGAGTGGGTGGAGGTGGTATCCGGCANGCAATGCCATAGTAGGCATCTNCCAGC 180
|||||||||||||||||||||||||||||||| ||||||||||||||||||||| |||||
7758R-1.in silico 121 CAAACACGAGTGGGTGGAGGTGGTATCCGGCA-GCAATGCCATAGTAGGCATCT-CCAGC 180
7758R-1.IR_full 181 TACGCCCAGGAGGCTCTCGGGGATGTGGTGTTCGCCCAACTCCCAGAACCCGGCACGGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 181 TACGCCCAGGAGGCTCTCGGGGATGTGGTGTTCGCCCAACTCCCAGAACCCGGCACGGAA 240
7758R-1.IR_full 241 CTCAAGCAGGATGACGAATGTGGGGCCCTGGAGAGCGTTAAAGCGGCTAGCGAGGTGTAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 241 CTCAAGCAGGATGACGAATGTGGGGCCCTGGAGAGCGTTAAAGCGGCTAGCGAGGTGTAT 300
7758R-1.IR_full 301 TCACCCGTTAGTGGCAAGGTAATTGAAAAGAATGCCGAGGTGGAGGACACACCGGCCCTA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 301 TCACCCGTTAGTGGCAAGGTAATTGAAAAGAATGCCGAGGTGGAGGACACACCGGCCCTA 360
7758R-1.IR_full 361 GTCAACAGCAGTTGCTATGAGAAGGGCTGGCTTTTCAAGGTGGACCTGAAGAATCCCAAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 361 GTCAACAGCAGTTGCTATGAGAAGGGCTGGCTTTTCAAGGTGGACCTGAAGAATCCCAAG 420
7758R-1.IR_full 421 GAACTCGAAGCCCTTATGACCGAGGATCAGTACAAAGCCTTCCTGAGCAGCAGTGGGGAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7758R-1.in silico 421 GAACTCGAAGCCCTTATGACCGAGGATCAGTACAAAGCCTTCCTGAGCAGCAGTGGGGAT 480
7758R-1.IR_full 481 CACTAGAACCAGCAGCTAATC 501
|||||||||||||||||||||
7758R-1.in silico 481 CACTAGAACCAGCAGCTAATC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_079473.2 |
CG7758-RA (ppl), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132178.1 |
CG12663-RA (CG12663), mRNA |
| 0 |
0 |
1 |
8 |
4 |
NM_142932.1 |
CG12492-RA (CG12492), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_139621.1 |
CG1265-RB (CG1265), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136627.1 |
CG13740-RA (CG13740), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_143299.2 |
CG3350-RA (bigmax), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_140561.3 |
CG5931-RA (CG5931), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132881.2 |
CG3415-RA (CG3415), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043289.1 |
CG34100-RA, transcript variant A (mld), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043290.1 |
CG34100-RB, transcript variant B (mld), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_136212.1 |
CG9323-RA (CG9323), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_001014500.1 |
CG33558-RA (CG33558), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_166503.1 |
CG6393-RD, transcript variant D (CG6393), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137788.2 |
CG6393-RA, transcript variant A (CG6393), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079065.3 |
CG15113-RA (5-HT1B), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079407.2 |
CG7446-RA (Grd), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_080038.2 |
CG3433-RA (Coprox), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_135569.2 |
CG7456-RA (CG7456), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_133162.1 |
CG12231-RA (CG12231), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_079797.2 |
CG6348-RA, transcript variant A (ro), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_170294.1 |
CG6348-RB, transcript variant B (ro), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_140029.2 |
CG4942-RA (CG4942), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_136864.2 |
CG13185-RA (CG13185), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
 |
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