NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7757R-3

Symbol CG7757 Full Name CG7757

CG No CG7757 Old CG No CG7757

Synonyms CG7757

Accession No (Link to NCBI) NM_140899.1

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAAGGATGGCGACGACCAGAA

Primer Seq. 3'

aatctagaggtaccCATCGTCCAGAATCAGGGGTT

Predicted Fragment Size

IR fragment full Seq

0001 CAAGNATGGC GACGACCAGA AATTGTCCAA AAAGCGCGCT GCCgNCTCAG ATTCCAAAAG 
0061 TGGTGGTGGA AGCGCCCTTG AAGCgNCCAA GAAATCCCGC TTCGATGTCC CGGAAAAGAA 
0121 TGGCGGCGGT GGTGAGAAGA AAGAAGAGGT CGCCGTGCCC GCCTCGCTGA GTTCCACGCA 
0181 GATCAAGCTG ATGATGGCCC ATGCCCAGCG GGAGATCGAG GAACGCAAGC GGGCCCTGAG 
0241 TAATCTAAGA GACAAGGATC CGCTGCTGGC GTCAGTTCCA TCGATCGGAA TGCCCGTCGC 
0301 CTTAGCCACT CAAGCGCTGG CGAAGAAGCC CACGCCCGAG GACTCGGAGA AGGCCAGGAA 
0361 GATTGCCGAG TTGCAGGCGC AGATCAGGGC CAAGCTTACT GGCAATTTGG CTAGTTTGAT 
0421 TCAGCCCACT gCGGTANCNG NCGccGCTNC AGCTGNTGCT CAAGcGCAGG AGCGACCCAA 
0481 ACCCCTGATT CTGGACGATG

in silico PCR Fragment

0001 CAAGGATGGC GACGACCAGA AATTGTCCAA AAAGCGCGCT GCCGCCTCAG ATTCCAAAAG 
0061 TGGTGGTGGA AGCGCCCTTG AAGCGCCCAA GAAATCCCGC TTCGATGTCC CGGAAAAGAA 
0121 TGGCGGCGGT GGTGAGAAGA AAGAAGAGGT CGCCGTGCCC GCCTCGCTGA GTTCCACGCA 
0181 GATCAAGCTG ATGATGGCCC ATGCCCAGCG GGAGATCGAG GAACGCAAGC GGGCCCTGAG 
0241 TAATCTAAGA GACAAGGATC CGCTGCTGGC GTCAGTTCCA TCGATCGGAA TGCCAGTTGC 
0301 CTTAGCCACT CAAGCGCTGG CGAAGAAGCC CACGCCCGAG GACTCGGAGA AGGCCAGGAA 
0361 GATTGCCGAG TTGCAGGCAC AGATCAGGGC CAAGCTTACT GGCAATTTGG CTAGTTTGAT 
0421 TCAGCCCACT GCGGTAGCGG CCGCCGCTGC AGCTGCTGCT CAAGCGCAGG AGCGACCCAA 
0481 ACCCCTGATT CTGGACGATG

Assemble Data


7757R-3.IR_full       1   CAAGNATGGCGACGACCAGAAATTGTCCAAAAAGCGCGCTGCCGNCTCAGATTCCAAAAG 60
                          |||| ||||||||||||||||||||||||||||||||||||||| |||||||||||||||
7757R-3.in silico     1   CAAGGATGGCGACGACCAGAAATTGTCCAAAAAGCGCGCTGCCGCCTCAGATTCCAAAAG 60


7757R-3.IR_full       61  TGGTGGTGGAAGCGCCCTTGAAGCGNCCAAGAAATCCCGCTTCGATGTCCCGGAAAAGAA 120
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
7757R-3.in silico     61  TGGTGGTGGAAGCGCCCTTGAAGCGCCCAAGAAATCCCGCTTCGATGTCCCGGAAAAGAA 120


7757R-3.IR_full       121 TGGCGGCGGTGGTGAGAAGAAAGAAGAGGTCGCCGTGCCCGCCTCGCTGAGTTCCACGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7757R-3.in silico     121 TGGCGGCGGTGGTGAGAAGAAAGAAGAGGTCGCCGTGCCCGCCTCGCTGAGTTCCACGCA 180


7757R-3.IR_full       181 GATCAAGCTGATGATGGCCCATGCCCAGCGGGAGATCGAGGAACGCAAGCGGGCCCTGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7757R-3.in silico     181 GATCAAGCTGATGATGGCCCATGCCCAGCGGGAGATCGAGGAACGCAAGCGGGCCCTGAG 240


7757R-3.IR_full       241 TAATCTAAGAGACAAGGATCCGCTGCTGGCGTCAGTTCCATCGATCGGAATGCCCGTCGC 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||| || ||
7757R-3.in silico     241 TAATCTAAGAGACAAGGATCCGCTGCTGGCGTCAGTTCCATCGATCGGAATGCCAGTTGC 300


7757R-3.IR_full       301 CTTAGCCACTCAAGCGCTGGCGAAGAAGCCCACGCCCGAGGACTCGGAGAAGGCCAGGAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7757R-3.in silico     301 CTTAGCCACTCAAGCGCTGGCGAAGAAGCCCACGCCCGAGGACTCGGAGAAGGCCAGGAA 360


7757R-3.IR_full       361 GATTGCCGAGTTGCAGGCGCAGATCAGGGCCAAGCTTACTGGCAATTTGGCTAGTTTGAT 420
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
7757R-3.in silico     361 GATTGCCGAGTTGCAGGCACAGATCAGGGCCAAGCTTACTGGCAATTTGGCTAGTTTGAT 420


7757R-3.IR_full       421 TCAGCCCACTGCGGTANCNGNCGCCGCTNCAGCTGNTGCTCAAGCGCAGGAGCGACCCAA 480
                          |||||||||||||||| | | ||||||| |||||| ||||||||||||||||||||||||
7757R-3.in silico     421 TCAGCCCACTGCGGTAGCGGCCGCCGCTGCAGCTGCTGCTCAAGCGCAGGAGCGACCCAA 480


7757R-3.IR_full       481 ACCCCTGATTCTGGACGATG 500
                          ||||||||||||||||||||
7757R-3.in silico     481 ACCCCTGATTCTGGACGATG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140899.1	CG7757-RA, transcript variant A (CG7757), mRNA
99.37	479	1	1	1	NM_168825.1	CG7757-RB, transcript variant B (CG7757), mRNA
0.41	2	4	5	67	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0.41	2	4	5	67	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0.41	2	3	2	4	NM_143178.1	CG5886-RA (CG5886), mRNA
0.2	1	4	7	10	NM_135207.2	CG11319-RA (CG11319), mRNA
0	0	5	10	28	NM_143700.3	CG17724-RA, transcript variant A (CG17724), mRNA
0	0	5	2	16	NM_206365.1	CG11551-RA (ind), mRNA
0	0	4	5	28	NM_167834.1	CG17090-RB, transcript variant B (CG17090), mRNA
0	0	4	5	28	NM_138194.2	CG17090-RA, transcript variant A (CG17090), mRNA
0	0	4	4	11	NM_141240.1	CG14656-RA (CG14656), mRNA
0	0	4	3	3	NM_135676.2	CG14940-RA (Pde1c), mRNA
0	0	3	16	61	NM_169696.1	CG3992-RA, transcript variant A (srp), mRNA
0	0	3	16	58	NM_169694.1	CG3992-RB, transcript variant B (srp), mRNA
0	0	3	12	35	NM_206506.1	CG7467-RC, transcript variant C (osa), mRNA
0	0	3	12	35	NM_079668.2	CG7467-RB, transcript variant B (osa), mRNA
0	0	3	12	35	NM_169775.1	CG7467-RA, transcript variant A (osa), mRNA
0	0	3	12	27	NM_169909.1	CG5460-RB, transcript variant B (H), mRNA
0	0	3	12	27	NM_169908.1	CG5460-RD, transcript variant D (H), mRNA
0	0	3	12	27	NM_169907.1	CG5460-RA, transcript variant A (H), mRNA
0	0	3	12	27	NM_079694.2	CG5460-RC, transcript variant C (H), mRNA
0	0	3	12	19	NM_078586.3	CG4070-RA, transcript variant A (Tis11), mRNA
0	0	3	12	19	NM_167333.2	CG4070-RB, transcript variant B (Tis11), mRNA
0	0	3	12	14	NM_001042816.1	CG9907-RC, transcript variant C (para), mRNA
0	0	3	12	14	NM_001042815.1	CG9907-RB, transcript variant B (para), mRNA
0	0	3	12	14	NM_078647.3	CG9907-RA, transcript variant A (para), mRNA
0	0	3	11	49	NM_132172.2	CG15478-RA (CG15478), mRNA
0	0	3	11	44	NM_001032019.1	CG3992-RD, transcript variant D (srp), mRNA
0	0	3	11	22	NM_001014462.1	CG3151-RG, transcript variant G (Rbp9), mRNA
0	0	3	11	21	NM_057589.2	CG3151-RA, transcript variant A (Rbp9), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance
++

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