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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7749R-1 
 Symbol fat2  Full Name fat2 
 CG No CG7749  Old CG No CG7749 
 Synonyms ftl, CG7749, DCad76E, Cad76E, CT23445, fat2 
 Accession No (Link to NCBI) NM_140914.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGACTTACGCCAAGGGAGTAT 
 Primer Seq. 3' 
aatctagaggtaccAAGCTGCTGCAGGAGAGTAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGACTTACGC CAAGGGAGTA TTGCATGAAA GACTGGCCGG CCTGAGAGTT GGCTTGAACG 
0061 CAGAGGTTAA GTATAGGATA ATTAGTGGCG ATAAGGAGAA GCTATTTAAG GCCGAGGAGA 
0121 AACTGGTCGG AGATTTTGCC TTCTTAGCGA TTCGAACGCG GACAAATAAC GTTGTGCTAA 
0181 ACAGAGAAAA AACTGAGGAA TACGTTATAA GAGTGAAGGC ACATGTACAT TTGCACGACC 
0241 GAAATGTATC AAGCTATGAA ACGGAGGCGA ATATCCACAT CAAAGTACTG GATCGCAATG 
0301 ACCTGAGTCC GCTGTTTTAT CCGACCCAGT ACACCGTTGT TATTCCGGAG GACACGCCCA 
0361 AATATCAAAG TATTTTAAAG GTCACAGCTG ACGATGCTGA CCTCGGCATC AATGGGGAAA 
0421 TCTACTACAG CCTCCTGATG GATAGTGAAT ACTTTGCTAT CCATCCAACA ACTGGCGAAA 
0481 TTACTCTCCT GCAGCAGCTT  
 in silico PCR Fragment 
0001 AGACTTACGC CAAGGGAGTA TTGCATGAAA GACTGGCCGG CCTGAGAGTT GGCTTGAACG 
0061 CAGAGGTTAA GTATAGGATA ATTAGTGGCG ATAAGGAGAA GCTATTTAAG GCCGAGGAGA 
0121 AACTGGTCGG AGATTTTGCC TTCTTAGCGA TTCGAACGCG GACAAATAAC GTTGTGCTAA 
0181 ACAGAGAAAA AACTGAGGAA TACGTTATAA GAGTGAAGGC ACATGTACAT TTGCACGACC 
0241 GAAATGTATC AAGCTATGAA ACGGAGGCGA ATATCCACAT CAAAGTACTG GATCGCAATG 
0301 ACCTGAGTCC GCTGTTTTAT CCGACCCAGT ACACCGTTGT TATTCCGGAG GACACGCCCA 
0361 AATATCAAAG TATTTTAAAG GTCACAGCTG ACGATGCTGA CCTCGGCATC AATGGGGAAA 
0421 TCTACTACAG CCTCCTGATG GATAGTGAAT ACTTTGCTAT CCATCCAACA ACTGGCGAAA 
0481 TTACTCTCCT GCAGCAGCTT  
 Assemble Data 

7749R-1.IR_full       1   AGACTTACGCCAAGGGAGTATTGCATGAAAGACTGGCCGGCCTGAGAGTTGGCTTGAACG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     1   AGACTTACGCCAAGGGAGTATTGCATGAAAGACTGGCCGGCCTGAGAGTTGGCTTGAACG 60


7749R-1.IR_full       61  CAGAGGTTAAGTATAGGATAATTAGTGGCGATAAGGAGAAGCTATTTAAGGCCGAGGAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     61  CAGAGGTTAAGTATAGGATAATTAGTGGCGATAAGGAGAAGCTATTTAAGGCCGAGGAGA 120


7749R-1.IR_full       121 AACTGGTCGGAGATTTTGCCTTCTTAGCGATTCGAACGCGGACAAATAACGTTGTGCTAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     121 AACTGGTCGGAGATTTTGCCTTCTTAGCGATTCGAACGCGGACAAATAACGTTGTGCTAA 180


7749R-1.IR_full       181 ACAGAGAAAAAACTGAGGAATACGTTATAAGAGTGAAGGCACATGTACATTTGCACGACC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     181 ACAGAGAAAAAACTGAGGAATACGTTATAAGAGTGAAGGCACATGTACATTTGCACGACC 240


7749R-1.IR_full       241 GAAATGTATCAAGCTATGAAACGGAGGCGAATATCCACATCAAAGTACTGGATCGCAATG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     241 GAAATGTATCAAGCTATGAAACGGAGGCGAATATCCACATCAAAGTACTGGATCGCAATG 300


7749R-1.IR_full       301 ACCTGAGTCCGCTGTTTTATCCGACCCAGTACACCGTTGTTATTCCGGAGGACACGCCCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     301 ACCTGAGTCCGCTGTTTTATCCGACCCAGTACACCGTTGTTATTCCGGAGGACACGCCCA 360


7749R-1.IR_full       361 AATATCAAAGTATTTTAAAGGTCACAGCTGACGATGCTGACCTCGGCATCAATGGGGAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     361 AATATCAAAGTATTTTAAAGGTCACAGCTGACGATGCTGACCTCGGCATCAATGGGGAAA 420


7749R-1.IR_full       421 TCTACTACAGCCTCCTGATGGATAGTGAATACTTTGCTATCCATCCAACAACTGGCGAAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7749R-1.in silico     421 TCTACTACAGCCTCCTGATGGATAGTGAATACTTTGCTATCCATCCAACAACTGGCGAAA 480


7749R-1.IR_full       481 TTACTCTCCTGCAGCAGCTT 500
                          ||||||||||||||||||||
7749R-1.in silico     481 TTACTCTCCTGCAGCAGCTT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_001031967.1  CG7749-RB, transcript variant B (fat2), mRNA 
100   482  0  0  1  NM_140914.2  CG7749-RA, transcript variant A (fat2), mRNA 
0   0  2  5  4  NM_168241.1  CG8114-RA, transcript variant A (pbl), mRNA 
0   0  2  5  4  NM_079241.2  CG8114-RB, transcript variant B (pbl), mRNA 
0   0  2  5  4  NM_168242.1  CG8114-RC, transcript variant C (pbl), mRNA 
0   0  2  5  4  NM_168243.1  CG8114-RD, transcript variant D (pbl), mRNA 
0   0  1  4  4  NM_143032.1  CG6980-RA (CG6980), mRNA 
0   0  1  3  5  NM_001043037.1  CG17800-PAU (Dscam), mRNA 
0   0  1  3  4  NM_134789.1  CG15356-RA (CG15356), mRNA 
0   0  1  3  2  NM_134642.2  CG11371-RB (dbr), mRNA 
0   0  1  2  3  NM_057579.3  CG12759-RA (Dbp45A), mRNA 
0   0  0  5  5  NM_057696.3  CG14396-RA, transcript variant A (Ret), mRNA 
0   0  0  5  5  NM_057697.3  CG14396-RD, transcript variant D (Ret), mRNA 
0   0  0  5  5  NM_165358.1  CG14396-RB, transcript variant B (Ret), mRNA 
0   0  0  5  5  NM_165360.1  CG14396-RE, transcript variant E (Ret), mRNA 
0   0  0  5  5  NM_165359.1  CG14396-RC, transcript variant C (Ret), mRNA 
0   0  0  5  3  NM_057523.3  CG18412-RA (ph-p), mRNA 
0   0  0  4  7  NM_141478.1  CG11286-RA (CG11286), mRNA 
0   0  0  4  7  NM_167956.1  CG1044-RB, transcript variant B (dos), mRNA 
0   0  0  4  7  NM_079166.2  CG1044-RA, transcript variant A (dos), mRNA 
0   0  0  4  6  NM_166909.2  CG14814-RA, transcript variant A (CG14814), mRNA 
0   0  0  4  6  NM_130591.2  CG14814-RB, transcript variant B (CG14814), mRNA 
0   0  0  4  3  NM_165317.1  CG10726-RB, transcript variant B (barr), mRNA 
0   0  0  4  3  NM_057758.3  CG10726-RA, transcript variant A (barr), mRNA 
0   0  0  4  3  NM_164957.1  CG6181-RB, transcript variant B (CG6181), mRNA 
0   0  0  4  2  NM_139462.1  CG13800-RA (CG13800), mRNA 
0   0  0  3  9  NM_001015405.1  CG17374-PA.3 (CG17374), mRNA 
0   0  0  3  6  NM_141341.2  CG1208-RA (CG1208), mRNA 
0   0  0  3  5  NM_140489.2  CG6778-RA, transcript variant A (Aats-gly), mRNA 
0   0  0  3  5  NM_168605.1  CG6778-RB, transcript variant B (Aats-gly), mRNA 

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