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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7725R-1 |
| Symbol |
rogdi |
Full Name |
rogdi |
| CG No |
CG7725 |
Old CG No |
CG7725 |
| Synonyms |
CG7725, rogdi |
| Accession No (Link to NCBI) |
NM_140699.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGCGTGAGGAAGCCCTCAAC |
| Primer Seq. 3' |
aatctagaggtaccCGCCCTTTGATCAGCTCATCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAGCGTGAGG AAGCCCTCAA CCTGCAAATC GAGTTCGAGT GGGTGCTGCG CCAAGAAGTA
0061 CACGCGATAC TGAAGCAATT AAGGAGCATT CTGGTGGAGT GCGCTCATCG ATTTCCGGTG
0121 CCCCTTTACG AGAACGAGGG AAAGAAGACT GAGAAGTTCA TACTCACCGT GTCGCCCGAT
0181 CAGCTGAAGG CCGTGCTGAC CCTAACGGGC GATGCCATCA CCCAGGCTGA CATTAGCTTT
0241 AAGCTGTGCA AGGCACCGAG CCAAACGCAA CGTACCTCCA TTACACATGA CTCTCCCTGG
0301 AAGTTGCAGC AGGTCCAGGA TGCGGCCAAT CATCTGCAGA CCGCCATCAA CCACATAGAC
0361 GATGTCGACG ACTCTTATCA CTTCAAAACC TCCGACGAAG TGCTGCATGT GATAGGCAAC
0421 ATTCTGGATG CCCTGCAGCG GGGCAGAAAC AGTTTGCTGG TGCCCAAAAA GAAGCCCATC
0481 GATGAGCTGA TCAAAGGGCG |
| in silico PCR Fragment |
0001 GAGCGTGAGG AAGCCCTCAA CCTGCAAATC GAGTTCGAGT GGGTGCTGCG CCAAGAAGTA
0061 CACGCGATAC TGAAGCAATT AAGGAGCATT CTGGTGGAGT GCGCTCATCG ATTTCCGGTG
0121 CCCCTTTACG AGAACGAGGG AAAGAAGACT GAGAAGTTCA TACTCACCGT GTCGCCCGAT
0181 CAGCTGAAGG CCGTGCTGAC CCTAACGGGC GATGCCATCA CCCAGGCTGA CATTAGCTTT
0241 AAGCTGTGCA AGGCACCGAG CCAAACGCAA CGTACCTCCA TTACACATGA CTCTCCCTGG
0301 AAGTTGCAGC AGGTCCAGGA TGCGGCCAAT CATCTGCAGA CCGCCATCAA CCACATAGAC
0361 GATGTCGACG ACTCTTATCA CTTCAAAACC TCCGACGAAG TGCTGCATGT GATAGGCAAC
0421 ATTCTGGATG CCCTGCAGCG GGGCAGAAAC AGTTTGCTGG TGCCCAAAAA GAAGCCCATC
0481 GATGAGCTGA TCAAAGGGCG |
| Assemble Data |
7725R-1.IR_full 1 GAGCGTGAGGAAGCCCTCAACCTGCAAATCGAGTTCGAGTGGGTGCTGCGCCAAGAAGTA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 1 GAGCGTGAGGAAGCCCTCAACCTGCAAATCGAGTTCGAGTGGGTGCTGCGCCAAGAAGTA 60
7725R-1.IR_full 61 CACGCGATACTGAAGCAATTAAGGAGCATTCTGGTGGAGTGCGCTCATCGATTTCCGGTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 61 CACGCGATACTGAAGCAATTAAGGAGCATTCTGGTGGAGTGCGCTCATCGATTTCCGGTG 120
7725R-1.IR_full 121 CCCCTTTACGAGAACGAGGGAAAGAAGACTGAGAAGTTCATACTCACCGTGTCGCCCGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 121 CCCCTTTACGAGAACGAGGGAAAGAAGACTGAGAAGTTCATACTCACCGTGTCGCCCGAT 180
7725R-1.IR_full 181 CAGCTGAAGGCCGTGCTGACCCTAACGGGCGATGCCATCACCCAGGCTGACATTAGCTTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 181 CAGCTGAAGGCCGTGCTGACCCTAACGGGCGATGCCATCACCCAGGCTGACATTAGCTTT 240
7725R-1.IR_full 241 AAGCTGTGCAAGGCACCGAGCCAAACGCAACGTACCTCCATTACACATGACTCTCCCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 241 AAGCTGTGCAAGGCACCGAGCCAAACGCAACGTACCTCCATTACACATGACTCTCCCTGG 300
7725R-1.IR_full 301 AAGTTGCAGCAGGTCCAGGATGCGGCCAATCATCTGCAGACCGCCATCAACCACATAGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 301 AAGTTGCAGCAGGTCCAGGATGCGGCCAATCATCTGCAGACCGCCATCAACCACATAGAC 360
7725R-1.IR_full 361 GATGTCGACGACTCTTATCACTTCAAAACCTCCGACGAAGTGCTGCATGTGATAGGCAAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 361 GATGTCGACGACTCTTATCACTTCAAAACCTCCGACGAAGTGCTGCATGTGATAGGCAAC 420
7725R-1.IR_full 421 ATTCTGGATGCCCTGCAGCGGGGCAGAAACAGTTTGCTGGTGCCCAAAAAGAAGCCCATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7725R-1.in silico 421 ATTCTGGATGCCCTGCAGCGGGGCAGAAACAGTTTGCTGGTGCCCAAAAAGAAGCCCATC 480
7725R-1.IR_full 481 GATGAGCTGATCAAAGGGCG 500
||||||||||||||||||||
7725R-1.in silico 481 GATGAGCTGATCAAAGGGCG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_168717.1 |
CG7725-RB, transcript variant B (rogdi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_140699.2 |
CG7725-RA, transcript variant A (rogdi), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_079582.2 |
CG6547-RA (mRpL37), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_140648.1 |
CG4169-RA (CG4169), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057785.3 |
CG5373-RA (Pi3K59F), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140460.1 |
CG13473-RA (CG13473), mRNA |
| 0 |
0 |
2 |
1 |
8 |
NM_141666.2 |
CG9492-RA (CG9492), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135852.2 |
CG4500-RA (CG4500), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135854.2 |
CG16876-RA (CG16876), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_206059.1 |
CG8083-RC, transcript variant C (CG8083), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_206060.1 |
CG8083-RB, transcript variant B (CG8083), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_136602.3 |
CG8083-RA, transcript variant A (CG8083), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137047.2 |
CG6197-RA (CG6197), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140383.1 |
CG10191-RA (CG10191), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141818.2 |
CG6744-RA (CG6744), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001042827.1 |
CG41482-RA (CG41482), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_167441.2 |
CG9176-RB, transcript variant B (cngl), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078608.3 |
CG9176-RC, transcript variant C (cngl), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169935.1 |
CG31191-RA (CG31191), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132992.2 |
CG8527-RA, transcript variant A (ppk23), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001014749.1 |
CG8527-RB, transcript variant B (ppk23), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132141.1 |
CG14431-RA (CG14431), mRNA |
| 0 |
0 |
0 |
11 |
4 |
NM_142835.2 |
CG4771-RA (CG4771), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_142349.2 |
CG4060-RA (CG4060), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_143717.1 |
CG10840-RB (eIF5B), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_170236.1 |
CG31096-RA (Lgr3), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132321.3 |
CG17754-RA, transcript variant A (CG17754), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167187.1 |
CG17754-RC, transcript variant C (CG17754), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_176721.1 |
CG17754-RD, transcript variant D (CG17754), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
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