NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7720R-4

Symbol CG7720 Full Name CG7720

CG No CG7720 Old CG No CG7720

Synonyms BcDNA:RH05973, CG7720

Accession No (Link to NCBI) NM_169834.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACCGTTTTGGGCCCGTTGTGG

Primer Seq. 3'

aatctagaggtaccTCCGCGTTGATAGCCGCCTTC

Predicted Fragment Size

IR fragment full Seq

0001 ACCGACCTTT TGGGCCCGTT GTGGAAACGC AtTTTTGGCA AAAAGAAGGA GCGCAGCAAG 
0061 GCCGACTATG TTTTTGCCAC CGGCGGAGTT TCGATAGTGG CAGTCATGAT TTCCATTGCC 
0121 CGAGGAACCT TGGGAGTGAG ATCCGTGCTA GGCNTATCCC AGTGAATTGT ACTACCGCGG 
0181 ATCGGGCCAT GTGGGAGATC ATCTACGGCA TGATGAGCGC CTATCCCATC GTTTGCTTCA 
0241 TGTTCGTGCC CGTGTACTTT AATCTGGGCA TCACCTCCGT CTATCAGTAC ATCGATCTCA 
0301 GGTTCAAGAG CCGCACGGTG AGATGCCTGG CATCGGCGAC ATATATCGTG CGGCAGATCT 
0361 GCAACCTTGG CATCACCGTG TACACTCCCA GCGTGGCCCT CTCGACGGTG ATTGGCATAC 
0421 CCTACTGGGC CTCCATTACG GGCATGGCCG TCATTTGCAT ATTCTTCACC ATTATGGGCG 
0481 GCCTGAAgGC GGCTATCAAC GCGGA

in silico PCR Fragment

0001 ACCG---TTT TGGGCCCGTT GTGGAAACGC ATTTTTGGCA AAAAGAAGGA GCGCAGCAAG 
0061 GCCGACTATG TTTTTGCCAC CGGCGGAGTT TCGATAGTGG CAGTCATGAT TTCCATTGCC 
0121 CGAGGAACCT TGGGAGTGAG ATCCGTGCTA GGC-TATCCC AGTGAATTGT ACTACCGCGG 
0181 ATC-GGCCAT GTGGGAGATC ATCTACGGCA TGATGAGCGC CTATCCCATC GTTTGCTTCA 
0241 TGTTCGTGCC CGTGTACTTT AATCTGGGCA TCACCTCCGT CTATCAGTAC ATCGATCTCA 
0301 GGTTCAAGAG CCGCACGGTG AGATGCCTGG CATCGGCGAC ATATATCGTG CGGCAGATCT 
0361 GCAACCTTGG CATCACCGTG TACACTCCCA GCGTGGCCCT CTCGACGGTG ATTGGCATAC 
0421 CCTACTGGGC CTCCATTACG GGCATGGCCG TCATTTGCAT ATTCTTCACC ATTATGGGCG 
0481 GCCTGAAGGC GGCTATCAAC GCGGA

Assemble Data


7720R-4.IR_full       1   ACCGACCTTTTGGGCCCGTTGTGGAAACGCATTTTTGGCAAAAAGAAGGAGCGCAGCAAG 60
                          ||||   |||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     1   ACCG---TTTTGGGCCCGTTGTGGAAACGCATTTTTGGCAAAAAGAAGGAGCGCAGCAAG 60


7720R-4.IR_full       61  GCCGACTATGTTTTTGCCACCGGCGGAGTTTCGATAGTGGCAGTCATGATTTCCATTGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     61  GCCGACTATGTTTTTGCCACCGGCGGAGTTTCGATAGTGGCAGTCATGATTTCCATTGCC 120


7720R-4.IR_full       121 CGAGGAACCTTGGGAGTGAGATCCGTGCTAGGCNTATCCCAGTGAATTGTACTACCGCGG 180
                          ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
7720R-4.in silico     121 CGAGGAACCTTGGGAGTGAGATCCGTGCTAGGC-TATCCCAGTGAATTGTACTACCGCGG 180


7720R-4.IR_full       181 ATCGGGCCATGTGGGAGATCATCTACGGCATGATGAGCGCCTATCCCATCGTTTGCTTCA 240
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     181 ATC-GGCCATGTGGGAGATCATCTACGGCATGATGAGCGCCTATCCCATCGTTTGCTTCA 240


7720R-4.IR_full       241 TGTTCGTGCCCGTGTACTTTAATCTGGGCATCACCTCCGTCTATCAGTACATCGATCTCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     241 TGTTCGTGCCCGTGTACTTTAATCTGGGCATCACCTCCGTCTATCAGTACATCGATCTCA 300


7720R-4.IR_full       301 GGTTCAAGAGCCGCACGGTGAGATGCCTGGCATCGGCGACATATATCGTGCGGCAGATCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     301 GGTTCAAGAGCCGCACGGTGAGATGCCTGGCATCGGCGACATATATCGTGCGGCAGATCT 360


7720R-4.IR_full       361 GCAACCTTGGCATCACCGTGTACACTCCCAGCGTGGCCCTCTCGACGGTGATTGGCATAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     361 GCAACCTTGGCATCACCGTGTACACTCCCAGCGTGGCCCTCTCGACGGTGATTGGCATAC 420


7720R-4.IR_full       421 CCTACTGGGCCTCCATTACGGGCATGGCCGTCATTTGCATATTCTTCACCATTATGGGCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7720R-4.in silico     421 CCTACTGGGCCTCCATTACGGGCATGGCCGTCATTTGCATATTCTTCACCATTATGGGCG 480


7720R-4.IR_full       481 GCCTGAAGGCGGCTATCAACGCGGA 505
                          |||||||||||||||||||||||||
7720R-4.in silico     481 GCCTGAAGGCGGCTATCAACGCGGA 505

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142496.2	CG7720-RB, transcript variant B (CG7720), mRNA
100	482	0	0	0	NM_169834.1	CG7720-RA, transcript variant A (CG7720), mRNA
0	0	2	2	4	NM_139783.1	CG10226-RA (CG10226), mRNA
0	0	2	2	3	NM_079065.3	CG15113-RA (5-HT1B), mRNA
0	0	2	2	2	NM_165723.2	CG15862-RB, transcript variant B (Pka-R2), mRNA
0	0	2	2	2	NM_078947.2	CG15862-RA, transcript variant A (Pka-R2), mRNA
0	0	1	5	9	NM_132641.1	CG15743-RA (CG15743), mRNA
0	0	1	5	3	NM_137477.2	CG5154-RA (Idgf5), mRNA
0	0	1	2	8	NM_141731.1	CG3996-RA (CG3996), mRNA
0	0	0	6	2	NM_164444.1	CG31663-RA (CG31663), mRNA
0	0	0	5	11	NM_137461.3	CG5140-RA (CG5140), mRNA
0	0	0	5	4	NM_132194.1	CG15329-RA (hdm), mRNA
0	0	0	4	7	NM_168384.1	CG6718-RC, transcript variant C (CG6718), mRNA
0	0	0	4	7	NM_168383.1	CG6718-RB, transcript variant B (CG6718), mRNA
0	0	0	4	7	NM_168385.1	CG6718-RD, transcript variant D (CG6718), mRNA
0	0	0	4	6	NM_139493.2	CG2083-RA (CG2083), mRNA
0	0	0	4	5	NM_140109.1	CG6718-RA, transcript variant A (CG6718), mRNA
0	0	0	4	4	NM_057944.3	CG12249-RB, transcript variant B (mira), mRNA
0	0	0	4	4	NM_135216.2	CG11070-RA (CG11070), mRNA
0	0	0	4	4	NM_057943.3	CG12249-RA, transcript variant A (mira), mRNA
0	0	0	4	3	NM_143343.2	CG4963-RA (CG4963), mRNA
0	0	0	3	6	NM_168512.1	CG32113-RA (CG32113), mRNA
0	0	0	3	6	NM_078929.2	CG11205-RA, transcript variant A (phr), mRNA
0	0	0	3	6	NM_165564.1	CG11205-RB, transcript variant B (phr), mRNA
0	0	0	3	5	NM_137003.3	CG4630-RA (CG4630), mRNA
0	0	0	3	4	NM_001043144.1	CG4998-RB, transcript variant B (CG4998), mRNA
0	0	0	3	4	NM_140621.1	CG4998-RA, transcript variant A (CG4998), mRNA
0	0	0	3	4	NM_137492.1	CG17669-RA (CG17669), mRNA
0	0	0	3	3	NM_142668.2	CG3822-RA (CG3822), mRNA
0	0	0	3	3	NM_134714.3	CG4341-RA (CG4341), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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