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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7719R-2 
 Symbol gwl  Full Name greatwall 
 CG No CG7719  Old CG No CG7719 
 Synonyms CG7719, Pk91C, EP0515, CG7719-related, CT23435, 1J, Pk?2, anon-WO2004063362.73, gwl 
 Accession No (Link to NCBI) NM_080121.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGATGTCCACATCGACTACAA 
 Primer Seq. 3' 
aatctagaggtaccTTCCGTGCTGATGCAGATACT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGATGTCCAC ATCGACTACA AAACCCCCAA GAAGACCCAC TCGCTGATCG ACAGTGAGCA 
0061 GTTGCTGGAC AAGATCAACA TCCTTACCAC CAAGCCGGAG AATCACTCCC AGAATGCCAA 
0121 GCTTCCCACT ATCAAGGATT TTGTCATCAT TAAGCCAATC AGCCGCGGCG CCTTTGGAAA 
0181 AGTCTTCCTG GGCTACAAGA ACAACGACTC GAAGCGGCTG TTCGCCATCA AGGTGATGCG 
0241 CAAGTCAGAG ATGATCAACA AGAACATGGT GTCCCAGGTA ATCACGGAGC GGAATGCCCT 
0301 GGCCCTCTCC CGCTCTCAGT TCTGTGTCAG TCTCTTCTAC TCCCTGCAAT CGCTCTCCTA 
0361 CGTCTACCTG GTGATGGAGT ACATGGTGGG CGGGGATCTC AAGTCACTGC TGGCCATGTT 
0421 TGGGTATTTT GACGAGCCCA CGGCCCGGTT CTACGTGGCC GAGATGGTGA TGGCGCTGCA 
0481 GTATCTGCAT CAGCACGGAA  
 in silico PCR Fragment 
0001 GGATGTCCAC ATCGACTACA AAACCCCCAA GAAGACCCAC TCGCTGATCG ACAGTGAGCA 
0061 GTTGCTGGAC AAGATCAACA TCCTTACCAC CAAGCCGGAG AATCACTCCC AGAATGCCAA 
0121 GCTTCCCACT ATCAAGGATT TTGTCATCAT TAAGCCAATC AGCCGCGGCG CCTTTGGAAA 
0181 AGTCTTCCTG GGCTACAAGA ACAACGACTC GAAGCGGCTG TTCGCCATCA AGGTGATGCG 
0241 CAAGTCAGAG ATGATCAACA AGAACATGGT GTCCCAGGTA ATCACGGAGC GGAATGCCCT 
0301 GGCCCTCTCC CGCTCTCAGT TCTGTGTCAG TCTCTTCTAC TCCCTGCAAT CGCTCTCCTA 
0361 CGTCTACCTG GTGATGGAGT ACATGGTGGG CGGGGATCTC AAGTCACTGC TGGCCATGTT 
0421 TGGGTATTTT GACGAGCCCA CGGCCCGGTT CTACGTGGCC GAGATGGTGA TGGCGCTGCA 
0481 GTATCTGCAT CAGCACGGAA  
 Assemble Data 

7719R-2.IR_full       1   GGATGTCCACATCGACTACAAAACCCCCAAGAAGACCCACTCGCTGATCGACAGTGAGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     1   GGATGTCCACATCGACTACAAAACCCCCAAGAAGACCCACTCGCTGATCGACAGTGAGCA 60


7719R-2.IR_full       61  GTTGCTGGACAAGATCAACATCCTTACCACCAAGCCGGAGAATCACTCCCAGAATGCCAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     61  GTTGCTGGACAAGATCAACATCCTTACCACCAAGCCGGAGAATCACTCCCAGAATGCCAA 120


7719R-2.IR_full       121 GCTTCCCACTATCAAGGATTTTGTCATCATTAAGCCAATCAGCCGCGGCGCCTTTGGAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     121 GCTTCCCACTATCAAGGATTTTGTCATCATTAAGCCAATCAGCCGCGGCGCCTTTGGAAA 180


7719R-2.IR_full       181 AGTCTTCCTGGGCTACAAGAACAACGACTCGAAGCGGCTGTTCGCCATCAAGGTGATGCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     181 AGTCTTCCTGGGCTACAAGAACAACGACTCGAAGCGGCTGTTCGCCATCAAGGTGATGCG 240


7719R-2.IR_full       241 CAAGTCAGAGATGATCAACAAGAACATGGTGTCCCAGGTAATCACGGAGCGGAATGCCCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     241 CAAGTCAGAGATGATCAACAAGAACATGGTGTCCCAGGTAATCACGGAGCGGAATGCCCT 300


7719R-2.IR_full       301 GGCCCTCTCCCGCTCTCAGTTCTGTGTCAGTCTCTTCTACTCCCTGCAATCGCTCTCCTA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     301 GGCCCTCTCCCGCTCTCAGTTCTGTGTCAGTCTCTTCTACTCCCTGCAATCGCTCTCCTA 360


7719R-2.IR_full       361 CGTCTACCTGGTGATGGAGTACATGGTGGGCGGGGATCTCAAGTCACTGCTGGCCATGTT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     361 CGTCTACCTGGTGATGGAGTACATGGTGGGCGGGGATCTCAAGTCACTGCTGGCCATGTT 420


7719R-2.IR_full       421 TGGGTATTTTGACGAGCCCACGGCCCGGTTCTACGTGGCCGAGATGGTGATGGCGCTGCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7719R-2.in silico     421 TGGGTATTTTGACGAGCCCACGGCCCGGTTCTACGTGGCCGAGATGGTGATGGCGCTGCA 480


7719R-2.IR_full       481 GTATCTGCATCAGCACGGAA 500
                          ||||||||||||||||||||
7719R-2.in silico     481 GTATCTGCATCAGCACGGAA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_080121.2  CG7719-RA (gwl), mRNA 
0.41   2  2  3  2  NM_141809.1  CG6715-RA (KP78a), mRNA 
0   0  3  4  4  NM_001042878.1  CG13784-RC, transcript variant C (CG13784), mRNA 
0   0  3  3  9  NM_140327.1  CG10522-RA (sti), mRNA 
0   0  2  8  9  NM_140521.1  CG6498-RA (CG6498), mRNA 
0   0  2  3  3  NM_206786.1  CG32549-RB, transcript variant B (CG32549), mRNA 
0   0  2  3  3  NM_206787.1  CG32549-RA, transcript variant A (CG32549), mRNA 
0   0  2  3  3  NM_206785.1  CG32549-RF, transcript variant F (CG32549), mRNA 
0   0  2  2  5  NM_166138.3  CG18250-RB, transcript variant B (Dg), mRNA 
0   0  2  2  2  NM_176184.2  CG18250-RC, transcript variant C (Dg), mRNA 
0   0  2  2  2  NM_079032.2  CG18250-RA, transcript variant A (Dg), mRNA 
0   0  1  4  5  NM_164843.1  CG9556-RA, transcript variant A (alien), mRNA 
0   0  1  4  5  NM_078793.4  CG9556-RB, transcript variant B (alien), mRNA 
0   0  1  4  2  NM_139514.2  CG11537-RC, transcript variant C (CG11537), mRNA 
0   0  1  3  5  NM_139934.3  CG8006-RA (CG8006), mRNA 
0   0  1  3  4  NM_130725.2  CG15239-RA (CG15239), mRNA 
0   0  1  3  3  NM_135558.2  CG5343-RA (CG5343), mRNA 
0   0  1  2  10  NM_135667.3  CG6614-RA (CG6614), mRNA 
0   0  1  2  5  NM_206184.1  CG13425-RC, transcript variant C (bl), mRNA 
0   0  1  2  5  NM_145883.2  CG13425-RB, transcript variant B (bl), mRNA 
0   0  1  2  5  NM_206185.1  CG13425-RA, transcript variant A (bl), mRNA 
0   0  1  2  5  NM_206183.1  CG13425-RD, transcript variant D (bl), mRNA 
0   0  1  2  5  NM_132981.1  CG8675-RA (CG8675), mRNA 
0   0  1  2  5  NM_079331.2  CG11280-RA (trn), mRNA 
0   0  1  2  4  NM_138985.2  CG8947-RA (26-29-p), mRNA 
0   0  1  2  4  NM_140687.4  CG6664-RA, transcript variant A (CG6664), mRNA 
0   0  1  2  4  NM_168710.3  CG6664-RC, transcript variant C (CG6664), mRNA 
0   0  1  2  4  NM_168709.3  CG6664-RB, transcript variant B (CG6664), mRNA 
0   0  1  2  4  NM_168711.2  CG6664-RD, transcript variant D (CG6664), mRNA 
0   0  1  2  3  NM_137414.2  CG4996-RA (CG4996), mRNA 

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