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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7679R-3 
 Symbol Vha100-2  Full Name Vha100-2 
 CG No CG18617  Old CG No CG7679 
 Synonyms CG7679, V-ATPase, vha100-2, CG18617, BcDNA:LD21735, anon-WO0118547.296, Vha100-2 
 Accession No (Link to NCBI) NM_169810.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Lim DH, Lee S, Choi MS, Han JY, Seong Y, Na D, Kwon YS, Lee YS.
The conserved microRNA miR-8-3p coordinates the expression of V-ATPase subunits to regulate ecdysone biosynthesis for Drosophila metamorphosis.
FASEB J (2020) 34(5) 6449-6465 [ PubMed ID = 32196731 ] [ RRC reference ]

Nagata R, Nakamura M, Sanaki Y, Igaki T.
Cell Competition Is Driven by Autophagy.
Dev Cell (2019) 51(1) 99-112.e4 [ PubMed ID = 31543447 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGGGGACATGTTCCGTAGT 
 Primer Seq. 3' 
aatctagaggtaccTGTTGAGCAGCAGCATCGTTG 
 Predicted Fragment Size 
491 
 IR fragment full Seq 
0001 ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
0061 gccGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
0121 AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
0181 CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCNC 
0241 GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
0301 CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
0361 TCCAACTATC TGGAGCTGAC CNAGCTGCGC AAGGTGCTGG AGAACNACGC AGGGCTTTCT 
0421 TCTCCGACCA GGAGGTTCTC AATCTGGACT CCTCCAACCG AGCTGGAGGA GACAACGATG 
0481 CTGCTGCTCA ACA 
 in silico PCR Fragment 
0001 ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
0061 GCCGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
0121 AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
0181 CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCCC 
0241 GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
0301 CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
0361 TCCAACTATC TGGAGCTGAC CGAGCTGCGC AAGGTGCTGG AGAAC-ACGC AGGGC-TTCT 
0421 TCTCCGACCA GGAGGTTCTC AATCTGGACT CCTCCAACCG AGCTGGAGGA GACAACGATG 
0481 CTGCTGCTCA ACA 
 Assemble Data 

7679R-3.IR_full       1   ATGGGGGACATGTTCCGTAGTGAGGAGATGGCACTCTGCCAGATGTTCATTCAGCCGGAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     1   ATGGGGGACATGTTCCGTAGTGAGGAGATGGCACTCTGCCAGATGTTCATTCAGCCGGAG 60


7679R-3.IR_full       61  GCCGCGTATACCTCCGTATCTGAGCTGGGCGAAACCGGCTGCGTGCAGTTCCGCGACTTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     61  GCCGCGTATACCTCCGTATCTGAGCTGGGCGAAACCGGCTGCGTGCAGTTCCGCGACTTG 120


7679R-3.IR_full       121 AATGTGAACGTGAACGCCTTCCAGCGCAAGTTCGTCACCGAGGTGCGTCGCTGCGATGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     121 AATGTGAACGTGAACGCCTTCCAGCGCAAGTTCGTCACCGAGGTGCGTCGCTGCGATGAG 180


7679R-3.IR_full       181 CTGGAGCGCAAGATCCGCTACATCGAGACGGAGATCAAGAAGGACGGCATCGTCCTGCNC 240
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
7679R-3.in silico     181 CTGGAGCGCAAGATCCGCTACATCGAGACGGAGATCAAGAAGGACGGCATCGTCCTGCCC 240


7679R-3.IR_full       241 GACATCCAGGATGACATTCCGCGTGCGCCCAATCCACGCGAGATCATCGATCTGGAGGCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     241 GACATCCAGGATGACATTCCGCGTGCGCCCAATCCACGCGAGATCATCGATCTGGAGGCG 300


7679R-3.IR_full       301 CATCTGGAGAAGACCGAGTCGGAGATGATCGAGCTGGCCCAGAACGAGGTGAACATGAAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     301 CATCTGGAGAAGACCGAGTCGGAGATGATCGAGCTGGCCCAGAACGAGGTGAACATGAAG 360


7679R-3.IR_full       361 TCCAACTATCTGGAGCTGACCNAGCTGCGCAAGGTGCTGGAGAACNACGCAGGGCTTTCT 420
                          ||||||||||||||||||||| ||||||||||||||||||||||| ||||||||| ||||
7679R-3.in silico     361 TCCAACTATCTGGAGCTGACCGAGCTGCGCAAGGTGCTGGAGAAC-ACGCAGGGC-TTCT 420


7679R-3.IR_full       421 TCTCCGACCAGGAGGTTCTCAATCTGGACTCCTCCAACCGAGCTGGAGGAGACAACGATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7679R-3.in silico     421 TCTCCGACCAGGAGGTTCTCAATCTGGACTCCTCCAACCGAGCTGGAGGAGACAACGATG 480


7679R-3.IR_full       481 CTGCTGCTCAACA 493
                          |||||||||||||
7679R-3.in silico     481 CTGCTGCTCAACA 493



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   473  0  0  0  NM_142465.1  CG18617-RB, transcript variant B (Vha100-2), mRNA 
100   473  0  0  0  NM_169810.1  CG18617-RA, transcript variant A (Vha100-2), mRNA 
1.05   5  25  31  46  NM_170391.1  CG1709-RC, transcript variant C (Vha100-1), mRNA 
1.05   5  25  31  46  NM_170393.1  CG1709-RF, transcript variant F (Vha100-1), mRNA 
1.05   5  25  31  46  NM_170394.1  CG1709-RG, transcript variant G (Vha100-1), mRNA 
1.05   5  25  31  46  NM_170395.1  CG1709-RB, transcript variant B (Vha100-1), mRNA 
1.05   5  25  31  46  NM_143415.2  CG1709-RE, transcript variant E (Vha100-1), mRNA 
1.05   5  25  31  46  NM_170392.1  CG1709-RA, transcript variant A (Vha100-1), mRNA 
1.05   5  25  31  46  NM_170396.1  CG1709-RD, transcript variant D (Vha100-1), mRNA 
1.05   5  25  31  38  NM_170397.1  CG1709-RH, transcript variant H (Vha100-1), mRNA 
0   0  5  36  64  NM_135671.1  CG12602-RA (CG12602), mRNA 
0   0  5  3  7  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  5  3  7  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  4  2  3  NM_142339.1  CG5169-RA (CG5169), mRNA 
0   0  3  12  34  NM_142463.2  CG7678-RA (CG7678), mRNA 
0   0  3  3  4  NM_143313.1  CG5639-RA (CG5639), mRNA 
0   0  3  3  4  NM_139588.3  CG12605-RB, transcript variant B (CG12605), mRNA 
0   0  3  3  4  NM_206275.2  CG12605-RA, transcript variant A (CG12605), mRNA 
0   0  3  2  5  NM_079395.2  CG9695-RA (Dab), mRNA 
0   0  2  6  6  NM_135462.2  CG13116-RA (CG13116), mRNA 
0   0  2  4  23  NM_169644.1  CG4843-RB, transcript variant B (Tm2), mRNA 
0   0  2  4  23  NM_169645.2  CG4843-RC, transcript variant C (Tm2), mRNA 
0   0  2  4  23  NM_079637.4  CG4843-RA, transcript variant A (Tm2), mRNA 
0   0  2  4  3  NM_140174.4  CG7839-RA (CG7839), mRNA 
0   0  2  4  2  NM_138202.2  CG17183-RA (MED30), mRNA 
0   0  2  4  2  NM_132228.2  CG10761-RA (CG10761), mRNA 
0   0  2  3  5  NM_142218.1  CG18522-RA (CG18522), mRNA 
0   0  2  2  2  NM_136362.2  CG8345-RA (Cyp6w1), mRNA 
0   0  1  6  22  NM_169061.1  CG8345-RA (Cyp6w1), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA 
0   0  1  6  22  NM_169060.1  CG8345-RA (Cyp6w1), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA 

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