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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7614R-1 
 Symbol Mat1  Full Name Mat1 
 CG No CG7614  Old CG No CG7614 
 Synonyms MAT1, CG7614, Mat1 
 Accession No (Link to NCBI) NM_136761.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGCGGTGCAAGACCACCAAAT 
 Primer Seq. 3' 
aatctagaggtaccCTTGTGCTCGTTCTCCAGTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGCGGTGCAA GACCACCAAA TACCGGAATC CATCGCTCAA GCTGATGGTC AACGTTTGCG 
0061 GACACACATT ATGCGAATCT TGCGTGGACT TGCTGTTCCT CAAAGGATCC GGCGCCTGTC 
0121 CAGAGTGCAT GGTCCCATTG CGGCGCAACA ACTTNCCGCG TGCAGCTTTT TGAGGACCCA 
0181 ATGGTGGAGA AGGAGGTGGA TATTCGTAGG CGAATTCTGC GCGACTACAA TAAACGTGAG 
0241 GAGGACTTTG CCTCCCTGGC GGAGTACAAT GACTATCTGG AGGAGATCGA GGACATTGTG 
0301 TACAATCTGT GCAACAACAT CGACATCATC GAGACAAACA AGCGTATTGA GGCCTACAAG 
0361 CGTGATAACC GTGAGGTCAT TCAGCGGAAT AAAACCCGCG TGGGACGCGA TGAGTACGCG 
0421 TTGGAGGAGA TGCTGGAGCT GGAGAAAAGT TCAGGAGGAG GCGCGTAGGA AGGAGCTGGA 
0481 GGAACTGGAG AACGAGCACA AG 
 in silico PCR Fragment 
0001 CGCGGTGCAA GACCACCAAA TACCGGAATC CATCGCTCAA GCTGATGGTC AACGTTTGCG 
0061 GACACACATT ATGCGAATCT TGCGTGGACT TGCTGTTCCT CAAAGGATCC GGCGCCTGTC 
0121 CAGAGTGCAT GGTCCCATTG CGGCGCAACA ACTT-CCGCG TGCAGCTTTT TGAGGACCCA 
0181 ATGGTGGAGA AGGAGGTGGA TATTCGTAGG CGAATTCTGC GCGACTACAA TAAACGTGAG 
0241 GAGGACTTTG CCTCCCTGGC GGAGTACAAT GACTATCTGG AGGAGATCGA GGACATTGTG 
0301 TACAATCTGT GCAACAACAT CGACATCATC GAGACAAACA AGCGTATTGA GGCCTACAAG 
0361 CGTGATAACC GTGAGGTCAT TCAGCGGAAT AAAACCCGCG TGGGACGCGA TGAGTACGCG 
0421 TTGGAGGAGA TGCTGGAGCT GGAG-AAAGT TCAGGAGGAG GCGCGTAGGA AGGAGCTGGA 
0481 GGAACTGGAG AACGAGCACA AG 
 Assemble Data 

7614R-1.IR_full       1   CGCGGTGCAAGACCACCAAATACCGGAATCCATCGCTCAAGCTGATGGTCAACGTTTGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     1   CGCGGTGCAAGACCACCAAATACCGGAATCCATCGCTCAAGCTGATGGTCAACGTTTGCG 60


7614R-1.IR_full       61  GACACACATTATGCGAATCTTGCGTGGACTTGCTGTTCCTCAAAGGATCCGGCGCCTGTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     61  GACACACATTATGCGAATCTTGCGTGGACTTGCTGTTCCTCAAAGGATCCGGCGCCTGTC 120


7614R-1.IR_full       121 CAGAGTGCATGGTCCCATTGCGGCGCAACAACTTNCCGCGTGCAGCTTTTTGAGGACCCA 180
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
7614R-1.in silico     121 CAGAGTGCATGGTCCCATTGCGGCGCAACAACTT-CCGCGTGCAGCTTTTTGAGGACCCA 180


7614R-1.IR_full       181 ATGGTGGAGAAGGAGGTGGATATTCGTAGGCGAATTCTGCGCGACTACAATAAACGTGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     181 ATGGTGGAGAAGGAGGTGGATATTCGTAGGCGAATTCTGCGCGACTACAATAAACGTGAG 240


7614R-1.IR_full       241 GAGGACTTTGCCTCCCTGGCGGAGTACAATGACTATCTGGAGGAGATCGAGGACATTGTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     241 GAGGACTTTGCCTCCCTGGCGGAGTACAATGACTATCTGGAGGAGATCGAGGACATTGTG 300


7614R-1.IR_full       301 TACAATCTGTGCAACAACATCGACATCATCGAGACAAACAAGCGTATTGAGGCCTACAAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     301 TACAATCTGTGCAACAACATCGACATCATCGAGACAAACAAGCGTATTGAGGCCTACAAG 360


7614R-1.IR_full       361 CGTGATAACCGTGAGGTCATTCAGCGGAATAAAACCCGCGTGGGACGCGATGAGTACGCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7614R-1.in silico     361 CGTGATAACCGTGAGGTCATTCAGCGGAATAAAACCCGCGTGGGACGCGATGAGTACGCG 420


7614R-1.IR_full       421 TTGGAGGAGATGCTGGAGCTGGAGAAAAGTTCAGGAGGAGGCGCGTAGGAAGGAGCTGGA 480
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
7614R-1.in silico     421 TTGGAGGAGATGCTGGAGCTGGAG-AAAGTTCAGGAGGAGGCGCGTAGGAAGGAGCTGGA 480


7614R-1.IR_full       481 GGAACTGGAGAACGAGCACAAG 502
                          ||||||||||||||||||||||
7614R-1.in silico     481 GGAACTGGAGAACGAGCACAAG 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136761.2  CG7614-RA (Mat1), mRNA 
0.41   2  2  2  5  NM_170557.1  CG11525-RD, transcript variant D (CycG), mRNA 
0.41   2  2  2  4  NM_170555.1  CG11525-RB, transcript variant B (CycG), mRNA 
0.41   2  2  2  4  NM_079870.2  CG11525-RA, transcript variant A (CycG), mRNA 
0.41   2  2  2  4  NM_170558.1  CG11525-RE, transcript variant E (CycG), mRNA 
0.41   2  2  2  4  NM_170556.1  CG11525-RC, transcript variant C (CycG), mRNA 
0.2   1  2  2  3  NM_169061.1  CG11525-RC, transcript variant C (CycG), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA 
0.2   1  2  2  3  NM_169060.1  CG11525-RC, transcript variant C (CycG), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA 
0   0  6  2  3  NM_132096.3  CG3342-RA (CG3342), mRNA 
0   0  4  2  10  NM_080100.4  CG5353-RB, transcript variant B (Aats-thr), mRNA 
0   0  4  2  10  NM_164994.1  CG5353-RA, transcript variant A (Aats-thr), mRNA 
0   0  4  2  10  NM_164995.1  CG5353-RC, transcript variant C (Aats-thr), mRNA 
0   0  4  2  8  NM_001038926.1  CG33261-RI, transcript variant I (Trl), mRNA 
0   0  4  2  8  NM_206356.2  CG33261-RB, transcript variant B (Trl), mRNA 
0   0  4  2  7  NM_135086.2  CG11024-RA (cl), mRNA 
0   0  3  7  14  NM_001042862.1  CG7337-RC, transcript variant C (CG7337), mRNA 
0   0  3  4  11  NM_165289.1  CG17549-RC, transcript variant C (CG17549), mRNA 
0   0  3  4  11  NM_165288.1  CG17549-RB, transcript variant B (CG17549), mRNA 
0   0  3  4  11  NM_136116.4  CG17549-RA, transcript variant A (CG17549), mRNA 
0   0  3  2  4  NM_140518.1  CG7439-RB, transcript variant B (AGO2), mRNA 
0   0  3  2  4  NM_168626.1  CG7439-RC, transcript variant C (AGO2), mRNA 
0   0  3  2  2  NM_140431.2  CG6451-RA (blue), mRNA 
0   0  2  7  2  NM_079159.2  CG1004-RA (rho), mRNA 
0   0  2  5  2  NM_132508.2  CG1703-RA (CG1703), mRNA 
0   0  2  4  3  NM_080194.3  CG4195-RA (l(3)73Ah), mRNA 
0   0  2  4  3  NM_143001.1  CG17781-RA (CG17781), mRNA 
0   0  2  4  3  NM_166322.1  CG16720-RB, transcript variant B (5-HT1A), mRNA 
0   0  2  4  3  NM_057454.3  CG16720-RA, transcript variant A (5-HT1A), mRNA 
0   0  2  2  11  NM_001042863.1  CG7337-RB, transcript variant B (CG7337), mRNA 
0   0  2  2  11  NM_134786.4  CG7337-RA, transcript variant A (CG7337), mRNA 

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