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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7604R-1 |
| Symbol |
Eig71Ee |
Full Name |
Ecdysone-induced gene 71Ee |
| CG No |
CG7604 |
Old CG No |
CG7604 |
| Synonyms |
eig71Ee, I71-7, gp150, CG7604, L71, VII, gene VII, gene-VII, L71-7, Eig71Ee |
| Accession No (Link to NCBI) |
NM_079368.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Korayem AM, Fabbri M, Takahashi K, Scherfer C, Lindgren M, Schmidt O, Ueda R, Dushay MS, Theopold U.
A Drosophila salivary gland mucin is also expressed in immune tissues: evidence for a function in coagulation and the entrapment of bacteria.
Insect Biochem Mol Biol (2004) 34(12) 1297-304
[
PubMed ID = 15544943
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTAACTGTGGTCTGCTTAGTG |
| Primer Seq. 3' |
aatctagaggtaccGGAGTAGTGCTCTCTGTACAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTAACTGTGG TCTGCTTAGT GGTAAGCTTC TTTCTGCTGC ACTATGCNGA GCACAGTGAT
0061 GCCTGCTTGG AGGTAATTGA GAAAGCGTTG GGTCTCCAAC CGTGCAATGA AGGTGGTCGC
0121 AACGAACACA GGGAGCCTCA CAGGGGTGGA CCAGGACCTG TGAGATCTAC TAGGAGGCGC
0181 GGGAGAATCC CTAGGAGACG TGAGACACCA AGACCAATAC ATCACAATAC CAGAGAACGG
0241 AGGCATCACA CAAAAACAAG GAAGCCTAGA AAACCCGTTC CTTGTATTAC GAAGAGGACG
0301 GAACCTCCAC CGGTTACAGA TTTTACTACT CGAAAATCCA ATCCACCATG CACTTGTACT
0361 GAAAGCACTA CTCGAAAGAC AAATCCGACT TGTACTTGTA CGGAAAGCAC TACGAAAAAG
0421 ACAAATCCGA CTTGTACTTG TACTGAAAGC ACTACTCGAA AGACAAACCC GACTTGTACT
0481 TGTACAGAGA GCACTACTCC |
| in silico PCR Fragment |
0001 CTAACTGTGG TCTGCTTAGT GGTAAGCTTC TTTCTGCTGC ACTATGCAGA GCACAGTGAT
0061 GCCTGCTTGG AGGTAATTGA GAAAGCGTTG GGTCTCCAAC CGTGCAATGA AGGTGGTCGC
0121 AACGAACACA GGGAGCCTCA CAGGGGTGGA CCAGGACCTG TGAGATCTAC TAGGAGGCGC
0181 GGGAGAATCC CTAGGAGACG TGAGACACCA AGACCAATAC ATCACAATAC CAGAGAACGG
0241 AGGCATCACA CAAAAACAAG GAAGCCTAGA AAACCCGTTC CTTGTATTAC GAAGAGGACG
0301 GAACCTCCAC CGGTTACAGA TTTTACTACT CGAAAATCCA ATCCACCATG CACTTGTACT
0361 GAAAGCACTA CTCGAAAGAC AAATCCGACT TGTACTTGTA CGGAAAGCAC TACGAAAAAG
0421 ACAAATCCGA CTTGTACTTG TACTGAAAGC ACTACTCGAA AGACAAACCC GACTTGTACT
0481 TGTACAGAGA GCACTACTCC |
| Assemble Data |
7604R-1.IR_full 1 CTAACTGTGGTCTGCTTAGTGGTAAGCTTCTTTCTGCTGCACTATGCNGAGCACAGTGAT 60
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
7604R-1.in silico 1 CTAACTGTGGTCTGCTTAGTGGTAAGCTTCTTTCTGCTGCACTATGCAGAGCACAGTGAT 60
7604R-1.IR_full 61 GCCTGCTTGGAGGTAATTGAGAAAGCGTTGGGTCTCCAACCGTGCAATGAAGGTGGTCGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 61 GCCTGCTTGGAGGTAATTGAGAAAGCGTTGGGTCTCCAACCGTGCAATGAAGGTGGTCGC 120
7604R-1.IR_full 121 AACGAACACAGGGAGCCTCACAGGGGTGGACCAGGACCTGTGAGATCTACTAGGAGGCGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 121 AACGAACACAGGGAGCCTCACAGGGGTGGACCAGGACCTGTGAGATCTACTAGGAGGCGC 180
7604R-1.IR_full 181 GGGAGAATCCCTAGGAGACGTGAGACACCAAGACCAATACATCACAATACCAGAGAACGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 181 GGGAGAATCCCTAGGAGACGTGAGACACCAAGACCAATACATCACAATACCAGAGAACGG 240
7604R-1.IR_full 241 AGGCATCACACAAAAACAAGGAAGCCTAGAAAACCCGTTCCTTGTATTACGAAGAGGACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 241 AGGCATCACACAAAAACAAGGAAGCCTAGAAAACCCGTTCCTTGTATTACGAAGAGGACG 300
7604R-1.IR_full 301 GAACCTCCACCGGTTACAGATTTTACTACTCGAAAATCCAATCCACCATGCACTTGTACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 301 GAACCTCCACCGGTTACAGATTTTACTACTCGAAAATCCAATCCACCATGCACTTGTACT 360
7604R-1.IR_full 361 GAAAGCACTACTCGAAAGACAAATCCGACTTGTACTTGTACGGAAAGCACTACGAAAAAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 361 GAAAGCACTACTCGAAAGACAAATCCGACTTGTACTTGTACGGAAAGCACTACGAAAAAG 420
7604R-1.IR_full 421 ACAAATCCGACTTGTACTTGTACTGAAAGCACTACTCGAAAGACAAACCCGACTTGTACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7604R-1.in silico 421 ACAAATCCGACTTGTACTTGTACTGAAAGCACTACTCGAAAGACAAACCCGACTTGTACT 480
7604R-1.IR_full 481 TGTACAGAGAGCACTACTCC 500
||||||||||||||||||||
7604R-1.in silico 481 TGTACAGAGAGCACTACTCC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 138.38 |
667 |
280 |
217 |
175 |
NM_079368.2 |
CG7604-RA (Eig71Ee), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_143027.2 |
CG5805-RA (CG5805), mRNA |
| 0 |
0 |
2 |
7 |
8 |
NM_170306.1 |
CG31077-RA (CG31077), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_167012.1 |
CG7035-RA, transcript variant A (Cbp80), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_080011.2 |
CG7035-RB, transcript variant B (Cbp80), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_137074.1 |
CG6704-RA (CG6704), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057346.3 |
CG3258-RA (ase), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_136869.2 |
CG13183-RA (CG13183), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_078554.2 |
CG16944-RA, transcript variant A (sesB), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167246.1 |
CG16944-RC, transcript variant C (sesB), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167248.1 |
CG16944-RB, transcript variant B (sesB), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167247.1 |
CG16944-RD, transcript variant D (sesB), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_079712.2 |
CG18402-RA (InR), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_001042986.1 |
CG17704-RF, transcript variant F (Nipped-B), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_001042987.1 |
CG17704-RE, transcript variant E (Nipped-B), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_136889.2 |
CG8878-RA, transcript variant A (CG8878), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_139943.1 |
CG7201-RA (CG7201), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_206099.1 |
CG8878-RB, transcript variant B (CG8878), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_205902.1 |
CG3304-RB, transcript variant B (CG3304), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_134926.2 |
CG3304-RA, transcript variant A (CG3304), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078751.3 |
CG3047-RA (Sgs1), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_206772.1 |
CG5172-RB, transcript variant B (CG5172), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170661.1 |
CG32508-RB, transcript variant B (shakB), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170660.1 |
CG32508-RA, transcript variant A (shakB), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132976.1 |
CG5172-RA, transcript variant A (CG5172), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_140031.1 |
CG4821-RA, transcript variant A (Tequila), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_206524.1 |
CG17269-RA (Fancd2), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_134821.1 |
CG4271-RA (CG4271), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
|
|
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