|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7571R-1 |
| Symbol |
Oatp74D |
Full Name |
Organic anion transporting polypeptide 74D |
| CG No |
CG7571 |
Old CG No |
CG7571 |
| Synonyms |
CG7571, oatp 74D, Oatp74D |
| Accession No (Link to NCBI) |
NM_140732.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Okamoto N, Yamanaka N.
Steroid Hormone Entry into the Brain Requires a Membrane Transporter in Drosophila.
Curr Biol (2020) 30(2) 359-366.e3
[
PubMed ID = 31928869
]
[
RRC reference
]
Okamoto N, Viswanatha R, Bittar R, Li Z, Haga-Yamanaka S, Perrimon N, Yamanaka N.
A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila.
Dev Cell (2018) 47(3) 294-305.e7
[
PubMed ID = 30293839
]
[
RRC reference
]
Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.
Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.
PLoS One (2017) 12(3) e0172951
[
PubMed ID = 28257485
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGACGAAGAGCAATGGCGAT |
| Primer Seq. 3' |
aatctagaggtaccACCTGGGCCGACAGCCGAAAA |
| Predicted Fragment Size |
496 |
| IR fragment full Seq |
0001 ATGACGAAGA GCAATGGCGA NTGTGGAGGC GGCAGCCCAG GTGCAATCTC TGGGCGGAAA
0061 GCCCAGCAAC GGACATGGCC AGCTGAATGG GAATGGCTAT CATCAGAACG GCGGACGCAG
0121 GGATTCCAGT CAAGCCTTCA CACCACTGCT GTCGCAGCAC AATAATGGCA CCACCAATGG
0181 CGAGGTGACC ACTCCGCCTC CGAGTACAGT GCTCTACGAG AGCACCCCGA GCAATAATAA
0241 CGAGTGGAAG GCCCCGGAGG ATCTGGGACA CCTGAAGAAT GGCCTGGGCA ACATACTGAG
0301 CAGTAATAAT AATGGCACCG GCAATGGGCA CAGTCTGAGC GAAAAGTATG CCCATGAACA
0361 GGCTCCCCTG ACCGGAGGTT ACAAGTTGCC ACCTCGCTCC AGTGAGTCCG AGGAATCCGA
0421 TTTCGATTCC GACCTCAATG GCGGCTCCTC CGCGGAATCC AGCTCCAGTT GCGGCCTTTT
0481 CGGCTGTCGG CCCAGGT |
| in silico PCR Fragment |
0001 ATGACGAAGA GCAATGGCGA -TGTGGAGGC GGCAGCCCAG GTGCAATCTC TGGGCGGAAA
0061 GCCCAGCAAC GGACATGGCC AGCTGAATGG GAATGGCTAT CATCAGAACG GCGGACGCAG
0121 GGATTCCAGT CAAGCCTTCA CACCACTGCT GTCGCAGCAC AATAATGGCA CCACCAATGG
0181 CGAGGTGACC ACTCCGCCTC CGAGTACAGT GCTCTACGAG AGCACCCCGA GCAATAATAA
0241 CGAGTGGAAG GCCCCGGAGG ATCTGGGACA CCTGAAGAAT GGCCTGGGCA ACATACTGAG
0301 CAGTAATAAT AATGGCACCG GCAATGGGCA CAGTCTGAGC GAAAAGTATG CCCATGAACA
0361 GGCTCCCCTG ACCGGAGGTT ACAAGTTGCC ACCTCGCTCC AGTGAGTCCG AGGAATCCGA
0421 TTTCGATTCC GACCTCAATG GCGGCTCCTC CGCGGAATCC AGCTCCAGTT GCGGCCTTTT
0481 CGGCTGTCGG CCCAGGT |
| Assemble Data |
7571R-1.IR_full 1 ATGACGAAGAGCAATGGCGANTGTGGAGGCGGCAGCCCAGGTGCAATCTCTGGGCGGAAA 60
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 1 ATGACGAAGAGCAATGGCGA-TGTGGAGGCGGCAGCCCAGGTGCAATCTCTGGGCGGAAA 60
7571R-1.IR_full 61 GCCCAGCAACGGACATGGCCAGCTGAATGGGAATGGCTATCATCAGAACGGCGGACGCAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 61 GCCCAGCAACGGACATGGCCAGCTGAATGGGAATGGCTATCATCAGAACGGCGGACGCAG 120
7571R-1.IR_full 121 GGATTCCAGTCAAGCCTTCACACCACTGCTGTCGCAGCACAATAATGGCACCACCAATGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 121 GGATTCCAGTCAAGCCTTCACACCACTGCTGTCGCAGCACAATAATGGCACCACCAATGG 180
7571R-1.IR_full 181 CGAGGTGACCACTCCGCCTCCGAGTACAGTGCTCTACGAGAGCACCCCGAGCAATAATAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 181 CGAGGTGACCACTCCGCCTCCGAGTACAGTGCTCTACGAGAGCACCCCGAGCAATAATAA 240
7571R-1.IR_full 241 CGAGTGGAAGGCCCCGGAGGATCTGGGACACCTGAAGAATGGCCTGGGCAACATACTGAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 241 CGAGTGGAAGGCCCCGGAGGATCTGGGACACCTGAAGAATGGCCTGGGCAACATACTGAG 300
7571R-1.IR_full 301 CAGTAATAATAATGGCACCGGCAATGGGCACAGTCTGAGCGAAAAGTATGCCCATGAACA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 301 CAGTAATAATAATGGCACCGGCAATGGGCACAGTCTGAGCGAAAAGTATGCCCATGAACA 360
7571R-1.IR_full 361 GGCTCCCCTGACCGGAGGTTACAAGTTGCCACCTCGCTCCAGTGAGTCCGAGGAATCCGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 361 GGCTCCCCTGACCGGAGGTTACAAGTTGCCACCTCGCTCCAGTGAGTCCGAGGAATCCGA 420
7571R-1.IR_full 421 TTTCGATTCCGACCTCAATGGCGGCTCCTCCGCGGAATCCAGCTCCAGTTGCGGCCTTTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7571R-1.in silico 421 TTTCGATTCCGACCTCAATGGCGGCTCCTCCGCGGAATCCAGCTCCAGTTGCGGCCTTTT 480
7571R-1.IR_full 481 CGGCTGTCGGCCCAGGT 497
|||||||||||||||||
7571R-1.in silico 481 CGGCTGTCGGCCCAGGT 497
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
478 |
0 |
4 |
11 |
NM_140732.1 |
CG7571-RA (Oatp74D), mRNA |
| 0.2 |
1 |
2 |
2 |
5 |
NM_057678.3 |
CG31240-RA (repo), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001032266.1 |
CG18431-RA, transcript variant A (CG18431), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001032264.1 |
CG4844-RB, transcript variant B (CG4844), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001032265.1 |
CG4844-RA, transcript variant A (CG4844), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135826.2 |
CG9008-RA, transcript variant A (CG9008), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_165055.2 |
CG9008-RB, transcript variant B (CG9008), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141566.1 |
CG8159-RA (CG8159), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_130604.2 |
CG17766-RA (CG17766), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_057241.2 |
CG7210-RB, transcript variant B (kel), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165242.1 |
CG7210-RA, transcript variant A (kel), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042992.1 |
CG17082-RD, transcript variant D (CG17082), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042994.1 |
CG17082-RE, transcript variant E (CG17082), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042991.1 |
CG17082-RF, transcript variant F (CG17082), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042989.1 |
CG17082-RA, transcript variant A (CG17082), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042993.1 |
CG17082-RB, transcript variant B (CG17082), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_001042990.1 |
CG17082-RC, transcript variant C (CG17082), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_057520.3 |
CG3497-RA (Su(H)), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_140958.1 |
CG13247-RA (CG13247), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001038755.1 |
CG14408-RC, transcript variant C (CG14408), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079617.3 |
CG7855-RA (timeout), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_143745.2 |
CG18389-RA (Eip93F), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_166842.1 |
CG32817-RA (CG32817), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138241.2 |
CG9192-RA (CG9192), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001014500.1 |
CG33558-RA (CG33558), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_133163.3 |
CG9397-RA (1.28), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_078545.2 |
CG12653-RA (btd), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166118.1 |
CG8256-RA, transcript variant A (l(2)k05713), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166119.1 |
CG8256-RB, transcript variant B (l(2)k05713), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057755.2 |
CG16874-RA (Vm32E), mRNA |
|
|
|
