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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7562R-2 |
| Symbol |
Trf |
Full Name |
TBP-related factor |
| CG No |
CG7562 |
Old CG No |
CG7562 |
| Synonyms |
TRF, TRF1, X70838, dTRF1, Trf1, CG7562, trf, Trf |
| Accession No (Link to NCBI) |
NM_057591.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTTTAAAGTCGCGGACGCTG |
| Primer Seq. 3' |
aatctagaggtaccTTCGTAGGAACTAAACTGCCC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACTTTAAAGT CGCGGACGCT GAAAGGGACA GGGATAATGT GGCTGCGACC AGcAATGCTG
0061 CCGCCAATCC GCACGCAGCC CTGCAGCCGC AACAGCCCGT TGCCCTGGTG GAGCCCAAGG
0121 ATGCACAACA TGAGATACGA TTGCAGAATA TCGTGGCCAC CTTCTCGGTG AACTGTGAAC
0181 TGGACCTCAA GGCAATCAAC TCGCGTACGA GGAACTCGGA GTANCTCGCC CAAGCGCTTT
0241 TCGTGGCGTC ATCATGCGAA TGCACTCGCC CAGGTGCACA GCGCTTAATC TTTCGAACGG
0301 GCAAGGTCAT CTGCACGGGT GCACGAAACG AGATTGAGGC GGACATTGGA TCGCGAAAGT
0361 TCGCACGCAT CCTGCAGAAG CTGGGATTCC CCGTAAAGTT CATGGAATAC AAGCTGCAGA
0421 ATATTGTGGC CACCGTTGAT CTGCGCNTTC CCCATCCGCC NTGGAGAACC TCAACCACGT
0481 GCACGGGCAG TTTAGTTCCT ACGAA |
| in silico PCR Fragment |
0001 ACTTTAAAGT CGCGGACGCT GAAAGGGACA GGGATAATGT GGCTGCGACC AGCAATGCTG
0061 CCGCCAATCC GCACGCAGCC CTGCAGCCGC AACAGCCCGT TGCCCTGGTG GAGCCCAAGG
0121 ATGCACAACA TGAGATACGA TTGCAGAATA TCGTGGCCAC CTTCTCGGTG AACTGTGAAC
0181 TGGACCTCAA GGCAATCAAC TCGCGTACGA GGAACTCGGA GTA-CTCGCC CAAGCGC-TT
0241 TCGTGGCGTC ATCATGCGAA TGCACTCGCC CAGGTGCACA GCGC-TAATC TTTCGAACGG
0301 GCAAGGTCAT CTGCACGGGT GCACGAAACG AGATTGAGGC GGACATTGGA TCGCGAAAGT
0361 TCGCACGCAT CCTGCAGAAG CTGGGATTCC CCGTAAAGTT CATGGAATAC AAGCTGCAGA
0421 ATATTGTGGC CACCGTTGAT CTGCGC-TTC CCCATCCGCC -TGGAGAACC TCAACCACGT
0481 GCACGGGCAG TTTAGTTCCT ACGAA |
| Assemble Data |
7562R-2.IR_full 1 ACTTTAAAGTCGCGGACGCTGAAAGGGACAGGGATAATGTGGCTGCGACCAGCAATGCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7562R-2.in silico 1 ACTTTAAAGTCGCGGACGCTGAAAGGGACAGGGATAATGTGGCTGCGACCAGCAATGCTG 60
7562R-2.IR_full 61 CCGCCAATCCGCACGCAGCCCTGCAGCCGCAACAGCCCGTTGCCCTGGTGGAGCCCAAGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7562R-2.in silico 61 CCGCCAATCCGCACGCAGCCCTGCAGCCGCAACAGCCCGTTGCCCTGGTGGAGCCCAAGG 120
7562R-2.IR_full 121 ATGCACAACATGAGATACGATTGCAGAATATCGTGGCCACCTTCTCGGTGAACTGTGAAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7562R-2.in silico 121 ATGCACAACATGAGATACGATTGCAGAATATCGTGGCCACCTTCTCGGTGAACTGTGAAC 180
7562R-2.IR_full 181 TGGACCTCAAGGCAATCAACTCGCGTACGAGGAACTCGGAGTANCTCGCCCAAGCGCTTT 240
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| ||
7562R-2.in silico 181 TGGACCTCAAGGCAATCAACTCGCGTACGAGGAACTCGGAGTA-CTCGCCCAAGCGC-TT 240
7562R-2.IR_full 241 TCGTGGCGTCATCATGCGAATGCACTCGCCCAGGTGCACAGCGCTTAATCTTTCGAACGG 300
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
7562R-2.in silico 241 TCGTGGCGTCATCATGCGAATGCACTCGCCCAGGTGCACAGCGC-TAATCTTTCGAACGG 300
7562R-2.IR_full 301 GCAAGGTCATCTGCACGGGTGCACGAAACGAGATTGAGGCGGACATTGGATCGCGAAAGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7562R-2.in silico 301 GCAAGGTCATCTGCACGGGTGCACGAAACGAGATTGAGGCGGACATTGGATCGCGAAAGT 360
7562R-2.IR_full 361 TCGCACGCATCCTGCAGAAGCTGGGATTCCCCGTAAAGTTCATGGAATACAAGCTGCAGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7562R-2.in silico 361 TCGCACGCATCCTGCAGAAGCTGGGATTCCCCGTAAAGTTCATGGAATACAAGCTGCAGA 420
7562R-2.IR_full 421 ATATTGTGGCCACCGTTGATCTGCGCNTTCCCCATCCGCCNTGGAGAACCTCAACCACGT 480
|||||||||||||||||||||||||| ||||||||||||| |||||||||||||||||||
7562R-2.in silico 421 ATATTGTGGCCACCGTTGATCTGCGC-TTCCCCATCCGCC-TGGAGAACCTCAACCACGT 480
7562R-2.IR_full 481 GCACGGGCAGTTTAGTTCCTACGAA 505
|||||||||||||||||||||||||
7562R-2.in silico 481 GCACGGGCAGTTTAGTTCCTACGAA 505
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
4 |
6 |
4 |
NM_057591.3 |
CG7562-RA (Trf), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_168563.1 |
CG32137-RB, transcript variant B (CG32137), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_168564.1 |
CG32137-RA, transcript variant A (CG32137), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_143320.2 |
CG18437-RA (CG18437), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167082.2 |
CG4095-RA (CG4095), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079507.2 |
CG2530-RA (corto), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167961.1 |
CG1244-RE, transcript variant E (CG1244), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_078930.2 |
CG18654-RA, transcript variant A (Dgk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165568.1 |
CG18654-RB, transcript variant B (Dgk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167962.1 |
CG1244-RF, transcript variant F (CG1244), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167960.1 |
CG1244-RD, transcript variant D (CG1244), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167958.1 |
CG1244-RB, transcript variant B (CG1244), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139476.1 |
CG1244-RA, transcript variant A (CG1244), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167959.1 |
CG1244-RC, transcript variant C (CG1244), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_142237.2 |
CG4606-RA (alpha-Man-IIb), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_078645.2 |
CG4252-RA (mei-41), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_170625.1 |
CG17034-RC, transcript variant C (CG17034), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_170624.1 |
CG17034-RB, transcript variant B (CG17034), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165984.1 |
CG17034-RA, transcript variant A (CG17034), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137029.2 |
CG17034-RD, transcript variant D (CG17034), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165725.2 |
CG12134-RB, transcript variant B (CG12134), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143128.2 |
CG10562-RA (CG10562), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141283.2 |
CG12162-RA, transcript variant A (CG12162), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169054.1 |
CG12162-RB, transcript variant B (CG12162), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134581.1 |
CG1518-RA (CG1518), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_138025.2 |
CG3121-RA (CG3121), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137794.2 |
CG11073-RA (CG11073), mRNA |
| 0 |
0 |
0 |
5 |
12 |
NM_143738.2 |
CG1851-RA (Ady43A), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_168647.1 |
CG32156-RA, transcript variant A (Mbs), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_168648.1 |
CG32156-RB, transcript variant B (Mbs), mRNA |
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