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NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7467R-1 
 Symbol osa  Full Name osa 
 CG No CG7467  Old CG No CG7467 
 Synonyms CG7467, OSA, eld, Osa, en(lz)4F/4H, p300, E(E2F)3C, l(3)04539, l(3)00090, l(3)j9C3, anon-WO0172774.126, anon-WO0118547.314, osa 
 Accession No (Link to NCBI) NM_169775.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCCGCAAACACAGCAGCAGCA 
 Primer Seq. 3' 
aatctagaggtaccGTGGAGGCTTTCCACCTGGCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCCNCAAACA CAGCAGCAGC AGCAAGGTGG CGCTCCCGcC CCTGCTGcCA CGCCCCCATC 
0061 GGCTGGAGCG GCTCCTGGTG CcgCTACACC GCCAACCTCG GGCCCGCCCA CACCCAACAA 
0121 CAACAGCAAC AACGGTAGCG ATCCCAGCAT CCAGCAGCAA CAACAAAATG TTGCCCCACA 
0181 TCCATACGGC GCACCACCGC CCCCAGGATN CTGGTCCTGG AGGACCACCA GGACCGGACC 
0241 CAGCTGCAGT CATGCACTAT CATCATCTGC ACCAGCAGCA ACAGCAGCAT CCGCCACCAC 
0301 CCCATATGCA GCAACAGCAG CACCACGGCG GACCTGCACC ACCACCGCCC GGAGGAGCAC 
0361 CTGAGCATGC GCCCGGTGTC AAGGAGGAGT ACACTCACTT GCCGCCGCCT CATCCACATC 
0421 CGGCGTATGG TCGCTACCAC GCCGATCCCA ACATGGATCC ATATCGCTAT GGCCAGcCGC 
0481 TGCCAGGTGG AAAGCCTCCA C 
 in silico PCR Fragment 
0001 GCCGCAAACA CAGCAGCAGC AGCAAGGTGG CGCTCCCGCC CCTGCTGCCA CGCCCCCATC 
0061 GGCTGGAGCG GCTCCTGGTG CCGCTACACC GCCAACCTCG GGCCCGCCCA CACCCAACAA 
0121 CAACAGCAAC AACGGTAGCG ATCCCAGCAT CCAGCAGCAA CAACAAAATG TTGCCCCACA 
0181 TCCATACGGC GCACCACCGC CCCCAGGAT- CTGGTCCTGG AGGACCACCA GGACCGGACC 
0241 CAGCTGCAGT CATGCACTAT CATCATCTGC ACCAGCAGCA ACAGCAGCAT CCGCCACCAC 
0301 CCCATATGCA GCAACAGCAG CACCACGGCG GACCTGCACC ACCACCGCCC GGAGGAGCAC 
0361 CTGAGCATGC GCCCGGTGTC AAGGAGGAGT ACACTCACTT GCCGCCGCCT CATCCACATC 
0421 CGGCGTATGG TCGCTACCAC GCCGATCCCA ACATGGATCC ATATCGCTAT GGCCAGCCGC 
0481 TGCCAGGTGG AAAGCCTCCA C 
 Assemble Data 

7467R-1.IR_full       1   GCCNCAAACACAGCAGCAGCAGCAAGGTGGCGCTCCCGCCCCTGCTGCCACGCCCCCATC 60
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     1   GCCGCAAACACAGCAGCAGCAGCAAGGTGGCGCTCCCGCCCCTGCTGCCACGCCCCCATC 60


7467R-1.IR_full       61  GGCTGGAGCGGCTCCTGGTGCCGCTACACCGCCAACCTCGGGCCCGCCCACACCCAACAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     61  GGCTGGAGCGGCTCCTGGTGCCGCTACACCGCCAACCTCGGGCCCGCCCACACCCAACAA 120


7467R-1.IR_full       121 CAACAGCAACAACGGTAGCGATCCCAGCATCCAGCAGCAACAACAAAATGTTGCCCCACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     121 CAACAGCAACAACGGTAGCGATCCCAGCATCCAGCAGCAACAACAAAATGTTGCCCCACA 180


7467R-1.IR_full       181 TCCATACGGCGCACCACCGCCCCCAGGATNCTGGTCCTGGAGGACCACCAGGACCGGACC 240
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
7467R-1.in silico     181 TCCATACGGCGCACCACCGCCCCCAGGAT-CTGGTCCTGGAGGACCACCAGGACCGGACC 240


7467R-1.IR_full       241 CAGCTGCAGTCATGCACTATCATCATCTGCACCAGCAGCAACAGCAGCATCCGCCACCAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     241 CAGCTGCAGTCATGCACTATCATCATCTGCACCAGCAGCAACAGCAGCATCCGCCACCAC 300


7467R-1.IR_full       301 CCCATATGCAGCAACAGCAGCACCACGGCGGACCTGCACCACCACCGCCCGGAGGAGCAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     301 CCCATATGCAGCAACAGCAGCACCACGGCGGACCTGCACCACCACCGCCCGGAGGAGCAC 360


7467R-1.IR_full       361 CTGAGCATGCGCCCGGTGTCAAGGAGGAGTACACTCACTTGCCGCCGCCTCATCCACATC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     361 CTGAGCATGCGCCCGGTGTCAAGGAGGAGTACACTCACTTGCCGCCGCCTCATCCACATC 420


7467R-1.IR_full       421 CGGCGTATGGTCGCTACCACGCCGATCCCAACATGGATCCATATCGCTATGGCCAGCCGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7467R-1.in silico     421 CGGCGTATGGTCGCTACCACGCCGATCCCAACATGGATCCATATCGCTATGGCCAGCCGC 480


7467R-1.IR_full       481 TGCCAGGTGGAAAGCCTCCAC 501
                          |||||||||||||||||||||
7467R-1.in silico     481 TGCCAGGTGGAAAGCCTCCAC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  7  41  170  NM_079668.2  CG7467-RB, transcript variant B (osa), mRNA 
100   482  7  41  170  NM_169775.1  CG7467-RA, transcript variant A (osa), mRNA 
100   482  6  34  153  NM_206506.1  CG7467-RC, transcript variant C (osa), mRNA 
0.82   4  14  20  46  NM_136542.1  CG30361-RA, transcript variant A (mXr), mRNA 
0.82   4  2  2  11  NM_078656.3  CG9108-RA (RSG7), mRNA 
0.62   3  10  31  112  NM_164516.1  CG8817-RA, transcript variant A (lilli), mRNA 
0.62   3  10  31  105  NM_078740.2  CG8817-RB, transcript variant B (lilli), mRNA 
0.62   3  9  27  97  NM_164517.1  CG8817-RC, transcript variant C (lilli), mRNA 
0.62   3  8  78  248  NM_167685.1  CG12701-RB, transcript variant B (CG12701), mRNA 
0.62   3  8  78  248  NM_134512.4  CG12701-RA, transcript variant A (CG12701), mRNA 
0.62   3  8  14  38  NM_168288.1  CG32029-RA (CG32029), mRNA 
0.41   2  57  449  1514  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
0.41   2  57  449  1514  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
0.41   2  57  449  1514  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
0.41   2  17  112  462  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
0.41   2  12  98  394  NM_169786.1  CG7847-RB, transcript variant B (sr), mRNA 
0.41   2  12  34  125  NM_140438.2  CG32139-RA (Sox21b), mRNA 
0.41   2  10  37  147  NM_080712.5  CG1693-RA, transcript variant A (tty), mRNA 
0.41   2  10  37  147  NM_167744.3  CG1693-RB, transcript variant B (tty), mRNA 
0.41   2  9  42  173  NM_132027.2  CG15772-RA (CG15772), mRNA 
0.41   2  6  19  79  NM_143352.1  CG12870-RA (CG12870), mRNA 
0.41   2  4  28  108  NM_079818.2  CG10002-RA (fkh), mRNA 
0.41   2  4  28  74  NM_080086.1  CG9930-RA (E5), mRNA 
0.41   2  4  17  60  NM_166894.1  CG11491-RA, transcript variant A (br), mRNA 
0.41   2  4  17  50  NM_080340.2  CG2302-RA (nAcRalpha-7E), mRNA 
0.41   2  4  4  21  NM_130538.2  CG11381-RA (CG11381), mRNA 
0.41   2  4  3  13  NM_167184.1  CG12665-RA (Obp8a), mRNA 
0.41   2  3  30  94  NM_001043240.1  CG34114-RB (CG34114), mRNA 
0.41   2  3  20  85  NM_165794.2  CG11895-RA (stan), mRNA 
0.41   2  3  18  66  NM_080531.2  CG5481-RA (lea), mRNA 

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