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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7405R-1 |
| Symbol |
CycH |
Full Name |
Cyclin H |
| CG No |
CG7405 |
Old CG No |
CG7405 |
| Synonyms |
142767_at, CG7405, CycH |
| Accession No (Link to NCBI) |
NM_079483.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Shih HT, Chen WY, Liu KY, Shih ZS, Chen YJ, Hsieh PC, Kuo KL, Huang KH, Hsu PH, Liu YW, Chan SP, Lee HH, Tsai YC, Wu JT.
dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.
PLoS Genet (2016) 12(9) e1006262
[
PubMed ID = 27588417
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTATCCTGTGAGCTCGCAA |
| Primer Seq. 3' |
aatctagaggtaccAAACCCTCGATGGGTCTGAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGTATCCTG TGAGCTCGCA AAAGAGGTCC TGGACATTCG CCAATGAGGG CCAGCTCATG
0061 GAGTTCCGCG TGGAGcAGAA CAGCAAGTAC ATCGAGTCGC ACGAGGAGGA GGCGCAGGGT
0121 CgNGACCTCA ATGAGCACTT TCTCACGTCG GCGGAGGAGC GcCTGTNTGC TGAAGCAGTA
0181 CGAGATCTAC CTGTTCGATT TCTGCCGCCG CTTCGAACCG ACGATGCCCA AGTGCGTTGT
0241 GGGCACGGCC TNTCCACTAC TTCAAGCGGT TCTATCTGAA CAACTCCCCC ATGGACTATC
0301 ACCCCAAGGA GATTCTAAGC CACATGCGTG TTCGTTGCCT GCAAAGATTG AGGAGTTCAA
0361 CGTGTCCATC AACCAAGGTT CGTAAAACAA CATCAAGGGG CGNACAGGAA CAAGGCCANC
0421 CGACATAGTG TTGTCCAATG AAATTACTGC TGATTGGACA GCTCAACTAC TACCTCAACC
0481 ATACACAATC CGGTTCAGAA CCCATCGAGG NNNT |
| in silico PCR Fragment |
0001 ATGTATCCTG TGAGCTCGCA AAAGAGGTCC TGGACATTCG CCAATGAGGG CCAGCTCATG
0061 GAGTTCCGCG TGGAGCAGAA CAGCAAGTAC ATCGAGTCGC ACGAGGAGGA GGCGCAGGGT
0121 CGCGACCTCA ATGAGCACTT TCTCACGTCG GCGGAGGAGC GCCTGT-TGC TGAAGCAGTA
0181 CGAGATCTAC CTGTTCGATT TCTGCCGCCG CTTCGAACCG ACGATGCCCA AGTGCGTTGT
0241 GGGCACGGCC T-TCCACTAC TTCAAGCGGT TCTATCTGAA CAACTCCCCC ATGGACTATC
0301 ACCCCAAGGA GATTCT-AGC CACATGCGTG TTCGTTGCCT GCAAAG-TTG AGGAGTTCAA
0361 CGTGTCCATC AACCA--GTT CGT-AAACAA CATCAA-GGG CG-ACAGGAA CAAGGCCA-C
0421 CGACATAGTG TTGTCCAATG -AATTACTGC TGATTGGACA GCTCAACTAC TACCTC-ACC
0481 ATACACAATC C-GTTCAG-A CCCATCGAGG GTTT |
| Assemble Data |
7405R-1.IR_full 1 ATGTATCCTGTGAGCTCGCAAAAGAGGTCCTGGACATTCGCCAATGAGGGCCAGCTCATG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7405R-1.in silico 1 ATGTATCCTGTGAGCTCGCAAAAGAGGTCCTGGACATTCGCCAATGAGGGCCAGCTCATG 60
7405R-1.IR_full 61 GAGTTCCGCGTGGAGCAGAACAGCAAGTACATCGAGTCGCACGAGGAGGAGGCGCAGGGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7405R-1.in silico 61 GAGTTCCGCGTGGAGCAGAACAGCAAGTACATCGAGTCGCACGAGGAGGAGGCGCAGGGT 120
7405R-1.IR_full 121 CGNGACCTCAATGAGCACTTTCTCACGTCGGCGGAGGAGCGCCTGTNTGCTGAAGCAGTA 180
|| ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
7405R-1.in silico 121 CGCGACCTCAATGAGCACTTTCTCACGTCGGCGGAGGAGCGCCTGT-TGCTGAAGCAGTA 180
7405R-1.IR_full 181 CGAGATCTACCTGTTCGATTTCTGCCGCCGCTTCGAACCGACGATGCCCAAGTGCGTTGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7405R-1.in silico 181 CGAGATCTACCTGTTCGATTTCTGCCGCCGCTTCGAACCGACGATGCCCAAGTGCGTTGT 240
7405R-1.IR_full 241 GGGCACGGCCTNTCCACTACTTCAAGCGGTTCTATCTGAACAACTCCCCCATGGACTATC 300
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
7405R-1.in silico 241 GGGCACGGCCT-TCCACTACTTCAAGCGGTTCTATCTGAACAACTCCCCCATGGACTATC 300
7405R-1.IR_full 301 ACCCCAAGGAGATTCTAAGCCACATGCGTGTTCGTTGCCTGCAAAGATTGAGGAGTTCAA 360
|||||||||||||||| ||||||||||||||||||||||||||||| |||||||||||||
7405R-1.in silico 301 ACCCCAAGGAGATTCT-AGCCACATGCGTGTTCGTTGCCTGCAAAG-TTGAGGAGTTCAA 360
7405R-1.IR_full 361 CGTGTCCATCAACCAAGGTTCGTAAAACAACATCAAGGGGCGNACAGGAACAAGGCCANC 420
||||||||||||||| |||||| |||||||||||| ||||| ||||||||||||||| |
7405R-1.in silico 361 CGTGTCCATCAACCA--GTTCGT-AAACAACATCAA-GGGCG-ACAGGAACAAGGCCA-C 420
7405R-1.IR_full 421 CGACATAGTGTTGTCCAATGAAATTACTGCTGATTGGACAGCTCAACTACTACCTCAACC 480
|||||||||||||||||||| ||||||||||||||||||||||||||||||||||| |||
7405R-1.in silico 421 CGACATAGTGTTGTCCAATG-AATTACTGCTGATTGGACAGCTCAACTACTACCTC-ACC 480
7405R-1.IR_full 481 ATACACAATCCGGTTCAGAACCCATCGAGGNNNT 514
||||||||||| |||||| ||||||||||| |
7405R-1.in silico 481 ATACACAATCC-GTTCAG-ACCCATCGAGGGTTT 514
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079483.2 |
CG7405-RA (CycH), mRNA |
| 0.2 |
1 |
3 |
5 |
3 |
NM_176545.1 |
CG4620-RA, transcript variant A (unk), mRNA |
| 0.2 |
1 |
3 |
5 |
3 |
NM_176546.1 |
CG4620-RB, transcript variant B (unk), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_143083.2 |
CG10238-RA (CG10238), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_057431.2 |
CG7171-RA (Uro), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_132660.2 |
CG1716-RA (CG1716), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137622.2 |
CG11788-RA (CG11788), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
8 |
2 |
NM_132437.1 |
CG15207-RA (CG15207), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_135471.1 |
CG13123-RA (CG13123), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_170234.1 |
CG17383-RC, transcript variant C (CG17383), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_170235.1 |
CG17383-RD, transcript variant D (CG17383), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_170233.1 |
CG17383-RA, transcript variant A (CG17383), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_143152.2 |
CG17383-RB, transcript variant B (CG17383), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136409.1 |
CG17002-RB (CG17002), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_140879.1 |
CG9392-RA (CG9392), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_137058.2 |
CG12295-RB (stj), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_079817.2 |
CG1842-RA (Dhc98D), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_058024.3 |
CG18642-RA (Bem46), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_206235.1 |
CG13921-RB, transcript variant B (CG13921), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_139399.1 |
CG13921-RA, transcript variant A (CG13921), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_079617.3 |
CG7855-RA (timeout), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_138985.2 |
CG8947-RA (26-29-p), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_137909.2 |
CG9882-RA (Art7), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135808.2 |
CG7110-RB (CG7110), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135201.1 |
CG9595-RA (osm-6), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_176711.2 |
CG1543-RB (Tbh), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_176715.1 |
CG7033-RC, transcript variant C (CG7033), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132296.2 |
CG7033-RA, transcript variant A (CG7033), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167176.1 |
CG7033-RB, transcript variant B (CG7033), mRNA |
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