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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7293R-1 |
| Symbol |
Klp68D |
Full Name |
Kinesin-like protein at 68D |
| CG No |
CG7293 |
Old CG No |
CG7293 |
| Synonyms |
CG7293, KIF3B, DmKlp68D, klp68d, KLP68D, KLP[[64D/68D]], KLP5, KLP-5, Klp5, Klp68D |
| Accession No (Link to NCBI) |
NM_079305.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Honjo K, Mauthner SE, Wang Y, Skene JHP, Tracey WD Jr.
Nociceptor-Enriched Genes Required for Normal Thermal Nociception.
Cell Rep (2016) 16(2) 295-303
[
PubMed ID = 27346357
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Mattie FJ, Stackpole MM, Stone MC, Clippard JR, Rudnick DA, Qiu Y, Tao J, Allender DL, Parmar M, Rolls MM.
Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites.
Curr Biol (2010) 20(24) 2169-77
[
PubMed ID = 21145742
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTCCGATGCAGGCCCATGA |
| Primer Seq. 3' |
aatctagaggtaccGGCGTGCAGATTGGGCACATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTCCGATG CAGGCCCATG AGCAACAGAG AACGCTCGGA AAGATCGCCG GAGGTGGTCA
0061 ACGTGTATCC GAATCGAGGC GTTGTGGAGC TGCAGAATGT GGTCGATGGC AATAAGGAGC
0121 AGCGCAAGGT TTTCACCTAC GATGCCGCCT ATGATGCGAG TGCAACGCAG ACGACGCTCT
0181 ACCATGAAGT GGTCTTTCCC CTAGTCAGTT CCGTTTTGGA GGGATTCAAC GGATGTATAT
0241 TTGCCTATGG ACAAACGGGC ACTGGTAAAA CCTTCACAAT GGAGGGAGTA CGCGGAAATG
0301 ACGAACTGAT GGGCATAATA CCGCGCACAT TCGAACAGAT CTGGCTGCAC ATTAATCGCA
0361 CCGAGAATTT CCAGTTTCTG GTCGATGTAT CCTACCTAGA GATTTATATG GAGGAGCTGC
0421 GCGATCTGCT AAAGCCCAAC TCTAAGCATT TGGAGGTGCG GGAACGTGGA TCGGGCGTTT
0481 ATGTGCCCAA TCTGCACGCC |
| in silico PCR Fragment |
0001 TGGTCCGATG CAGGCCCATG AGCAACAGAG AACGCTCGGA AAGATCGCCG GAGGTGGTCA
0061 ACGTGTATCC GAATCGAGGC GTTGTGGAGC TGCAGAATGT GGTCGATGGC AATAAGGAGC
0121 AGCGCAAGGT TTTCACCTAC GATGCCGCCT ATGATGCGAG TGCAACGCAG ACGACGCTCT
0181 ACCATGAAGT GGTCTTTCCC CTAGTCAGTT CCGTTTTGGA GGGATTCAAC GGATGTATAT
0241 TTGCCTATGG ACAAACGGGC ACTGGTAAAA CCTTCACAAT GGAGGGAGTA CGCGGAAATG
0301 ACGAACTGAT GGGCATAATA CCGCGCACAT TCGAACAGAT CTGGCTGCAC ATTAATCGCA
0361 CCGAGAATTT CCAGTTTCTG GTCGATGTAT CCTACCTAGA GATTTATATG GAGGAGCTGC
0421 GCGATCTGCT AAAGCCCAAC TCTAAGCATT TGGAGGTGCG GGAACGTGGA TCGGGCGTTT
0481 ATGTGCCCAA TCTGCACGCC |
| Assemble Data |
7293R-1.IR_full 1 TGGTCCGATGCAGGCCCATGAGCAACAGAGAACGCTCGGAAAGATCGCCGGAGGTGGTCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 1 TGGTCCGATGCAGGCCCATGAGCAACAGAGAACGCTCGGAAAGATCGCCGGAGGTGGTCA 60
7293R-1.IR_full 61 ACGTGTATCCGAATCGAGGCGTTGTGGAGCTGCAGAATGTGGTCGATGGCAATAAGGAGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 61 ACGTGTATCCGAATCGAGGCGTTGTGGAGCTGCAGAATGTGGTCGATGGCAATAAGGAGC 120
7293R-1.IR_full 121 AGCGCAAGGTTTTCACCTACGATGCCGCCTATGATGCGAGTGCAACGCAGACGACGCTCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 121 AGCGCAAGGTTTTCACCTACGATGCCGCCTATGATGCGAGTGCAACGCAGACGACGCTCT 180
7293R-1.IR_full 181 ACCATGAAGTGGTCTTTCCCCTAGTCAGTTCCGTTTTGGAGGGATTCAACGGATGTATAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 181 ACCATGAAGTGGTCTTTCCCCTAGTCAGTTCCGTTTTGGAGGGATTCAACGGATGTATAT 240
7293R-1.IR_full 241 TTGCCTATGGACAAACGGGCACTGGTAAAACCTTCACAATGGAGGGAGTACGCGGAAATG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 241 TTGCCTATGGACAAACGGGCACTGGTAAAACCTTCACAATGGAGGGAGTACGCGGAAATG 300
7293R-1.IR_full 301 ACGAACTGATGGGCATAATACCGCGCACATTCGAACAGATCTGGCTGCACATTAATCGCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 301 ACGAACTGATGGGCATAATACCGCGCACATTCGAACAGATCTGGCTGCACATTAATCGCA 360
7293R-1.IR_full 361 CCGAGAATTTCCAGTTTCTGGTCGATGTATCCTACCTAGAGATTTATATGGAGGAGCTGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 361 CCGAGAATTTCCAGTTTCTGGTCGATGTATCCTACCTAGAGATTTATATGGAGGAGCTGC 420
7293R-1.IR_full 421 GCGATCTGCTAAAGCCCAACTCTAAGCATTTGGAGGTGCGGGAACGTGGATCGGGCGTTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7293R-1.in silico 421 GCGATCTGCTAAAGCCCAACTCTAAGCATTTGGAGGTGCGGGAACGTGGATCGGGCGTTT 480
7293R-1.IR_full 481 ATGTGCCCAATCTGCACGCC 500
||||||||||||||||||||
7293R-1.in silico 481 ATGTGCCCAATCTGCACGCC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079305.2 |
CG7293-RA (Klp68D), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_140607.1 |
CG13045-RA (CG13045), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_132559.1 |
CG2750-RA (CG2750), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_143363.1 |
CG1894-RA (CG1894), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_138263.1 |
CG9169-RA (FucTD), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_132582.1 |
CG1824-RA (CG1824), mRNA |
| 0 |
0 |
2 |
8 |
7 |
NM_079210.1 |
CG10642-RA (Klp64D), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_140715.2 |
CG7692-RA (CG7692), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_175954.1 |
CG33123-RA (CG33123), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_134708.2 |
CG13949-RA (CG13949), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176751.1 |
CG8557-RB, transcript variant B (CG8557), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132990.2 |
CG8557-RA, transcript variant A (CG8557), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_143609.2 |
CG31002-RA (CG31002), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130617.2 |
CG4281-RA (CG4281), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143022.2 |
CG13627-RA, transcript variant A (CG13627), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170166.2 |
CG13627-RB, transcript variant B (CG13627), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142740.1 |
CG6690-RA (CG6690), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_130690.1 |
CG3603-RA (CG3603), mRNA |
| 0 |
0 |
0 |
7 |
22 |
NM_057303.4 |
CG7831-RA (ncd), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_139668.1 |
CG15876-RA (CG15876), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_137664.1 |
CG9025-RA, transcript variant A (CG9025), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166408.1 |
CG9025-RB, transcript variant B (CG9025), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_058013.3 |
CG7393-RA (p38b), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057294.4 |
CG11680-RB, transcript variant B (mle), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057293.3 |
CG11680-RA, transcript variant A (mle), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_165451.2 |
CG11680-RC, transcript variant C (mle), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_168448.1 |
CG32082-RA (CG32082), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206458.2 |
CG17816-RD, transcript variant D (CG17816), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132773.2 |
CG5627-RA (rab3-GEF), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_137177.2 |
CG11808-RA (CG11808), mRNA |
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