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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7230R-4 
 Symbol rib  Full Name ribbon 
 CG No CG7230  Old CG No CG7230 
 Synonyms CG7230, gene 5, rib 
 Accession No (Link to NCBI) NM_080534.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Strutt H, Searle E, Thomas-Macarthur V, Brookfield R, Strutt D.
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Development (2013) 140(8) 1693-702 [ PubMed ID = 23487316 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTACAGATTCTACACGCCCTGC 
 Primer Seq. 3' 
aatctagaggtaccATGGCTGTAGCCCGGCTGATA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TACAGATTCT ACACGCCCTG CACGAGGTGG GCAGCTATGT GGACTGCAGC CTGGTGGTGG 
0061 ACGATGAACA GTTCCAGGCC CATCGCGTGG TTCTGGCGGC CAATTCACCG TATTTCCAGC 
0121 ACNATCCTGA AGGATGTGCC GCAGGACCAC TGCAGCATCA TACTGCCGGG AGTGAAGGGC 
0181 TTCGAGATTG CCGCTTTGCT GCAGTACATG TACACGGGCG AGACGACGGT GACCAAGAGC 
0241 CAGGAGCCGG AGATTCTGCG CACAGCCAAG GAGCTTCAGG TCAAGGGTCT GTACGACAAC 
0301 TTGATGAAGT TCAATCATGC CAGCGTCACG CCCACCTCCA GTTCGGGAGC TGGCGGAgNC 
0361 AAGCNCCCAG AACGGATCAG CATCCAACCA CTCCTCATCC GTGATCTCCA CATCCACGCA 
0421 CATCTCGCCC AGTGCCGCCA TCTCGAGCAG CTGCTCGCCA CCACCGCCGC CACAGTTTGG 
0481 CTATCAGcCG GGCTACAGCA TGGTACAT 
 in silico PCR Fragment 
0001 TACAGATTCT ACACGCCCTG CACGAGGTGG GCAGCTATGT GGACTGCAGC CTGGTGGTGG 
0061 ACGATGAACA GTTCCAGGCC CATCGCGTGG TTCTGGCGGC CAATTCACCG TATTTCCAGC 
0121 AC-ATCCTGA AGGATGTGCC GCAGGACCAC TGCAGCATCA TACTGCCGGG AGTGAAGGGC 
0181 TTCGAGATTG CCGCTTTGCT GCAGTACATG TACACGGGCG AGACGACGGT GACCAAGAGC 
0241 CAGGAGCCGG AGATTCTGCG CACAGCCAAG GAGCTTCAGG TCAAGGGTCT GTACGACAAC 
0301 TTGATGAAGT TCAATCATGC CAGCGTCACG CCCACCTCCA GTTCGGGAGC TGGCGGAGCC 
0361 AAGC-CCCAG AACGGATCAG CATCCAACCA CTCCTCATCC GTGATCTCCA CATCCACGCA 
0421 CATCTCGCCC AGTGCCGCCA TCTCGAGCAG CTGCTCGCCA CCACCGCCGC CGCAGTTTGG 
0481 CTATCAGCCG GGCTACAGC- -----CAT 
 Assemble Data 

7230R-4.IR_full       1   TACAGATTCTACACGCCCTGCACGAGGTGGGCAGCTATGTGGACTGCAGCCTGGTGGTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     1   TACAGATTCTACACGCCCTGCACGAGGTGGGCAGCTATGTGGACTGCAGCCTGGTGGTGG 60


7230R-4.IR_full       61  ACGATGAACAGTTCCAGGCCCATCGCGTGGTTCTGGCGGCCAATTCACCGTATTTCCAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     61  ACGATGAACAGTTCCAGGCCCATCGCGTGGTTCTGGCGGCCAATTCACCGTATTTCCAGC 120


7230R-4.IR_full       121 ACNATCCTGAAGGATGTGCCGCAGGACCACTGCAGCATCATACTGCCGGGAGTGAAGGGC 180
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     121 AC-ATCCTGAAGGATGTGCCGCAGGACCACTGCAGCATCATACTGCCGGGAGTGAAGGGC 180


7230R-4.IR_full       181 TTCGAGATTGCCGCTTTGCTGCAGTACATGTACACGGGCGAGACGACGGTGACCAAGAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     181 TTCGAGATTGCCGCTTTGCTGCAGTACATGTACACGGGCGAGACGACGGTGACCAAGAGC 240


7230R-4.IR_full       241 CAGGAGCCGGAGATTCTGCGCACAGCCAAGGAGCTTCAGGTCAAGGGTCTGTACGACAAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     241 CAGGAGCCGGAGATTCTGCGCACAGCCAAGGAGCTTCAGGTCAAGGGTCTGTACGACAAC 300


7230R-4.IR_full       301 TTGATGAAGTTCAATCATGCCAGCGTCACGCCCACCTCCAGTTCGGGAGCTGGCGGAGNC 360
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
7230R-4.in silico     301 TTGATGAAGTTCAATCATGCCAGCGTCACGCCCACCTCCAGTTCGGGAGCTGGCGGAGCC 360


7230R-4.IR_full       361 AAGCNCCCAGAACGGATCAGCATCCAACCACTCCTCATCCGTGATCTCCACATCCACGCA 420
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
7230R-4.in silico     361 AAGC-CCCAGAACGGATCAGCATCCAACCACTCCTCATCCGTGATCTCCACATCCACGCA 420


7230R-4.IR_full       421 CATCTCGCCCAGTGCCGCCATCTCGAGCAGCTGCTCGCCACCACCGCCGCCACAGTTTGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
7230R-4.in silico     421 CATCTCGCCCAGTGCCGCCATCTCGAGCAGCTGCTCGCCACCACCGCCGCCGCAGTTTGG 480


7230R-4.IR_full       481 CTATCAGCCGGGCTACAGCATGGTACAT 508
                          |||||||||||||||||||      |||
7230R-4.in silico     481 CTATCAGCCGGGCTACAGC------CAT 508



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  2  4  NM_080534.3  CG7230-RA (rib), mRNA 
0.2   1  3  2  7  NM_166106.1  CG8205-RA, transcript variant A (fus), mRNA 
0.2   1  3  2  7  NM_166105.1  CG8205-RC, transcript variant C (fus), mRNA 
0.2   1  2  5  2  NM_134619.2  CG14613-RA (CG14613), mRNA 
0.2   1  2  3  2  NM_133098.2  CG7053-RA (CG7053), mRNA 
0.2   1  2  2  7  NM_165034.1  CG6043-RA, transcript variant A (CG6043), mRNA 
0.2   1  2  2  7  NM_165037.1  CG6043-RC, transcript variant C (CG6043), mRNA 
0.2   1  2  2  7  NM_165035.1  CG6043-RB, transcript variant B (CG6043), mRNA 
0.2   1  2  2  7  NM_135785.1  CG6043-RD, transcript variant D (CG6043), mRNA 
0.2   1  2  2  7  NM_135957.2  CG31738-RB, transcript variant B (CG31738), mRNA 
0.2   1  2  2  7  NM_165168.1  CG31738-RA, transcript variant A (CG31738), mRNA 
0   0  5  2  13  NM_168203.2  CG8597-RC, transcript variant C (lark), mRNA 
0   0  5  2  10  NM_168202.2  CG8597-RB, transcript variant B (lark), mRNA 
0   0  5  2  10  NM_079233.4  CG8597-RA, transcript variant A (lark), mRNA 
0   0  5  2  10  NM_168205.2  CG8597-RE, transcript variant E (lark), mRNA 
0   0  5  2  7  NM_168204.2  CG8597-RD, transcript variant D (lark), mRNA 
0   0  4  5  9  NM_166896.1  CG11491-RB, transcript variant B (br), mRNA 
0   0  4  5  9  NM_166897.1  CG11491-RC, transcript variant C (br), mRNA 
0   0  4  2  6  NM_132610.2  CG4404-RA (CG4404), mRNA 
0   0  3  9  10  NM_164449.1  CG4244-RC, transcript variant C (Su(dx)), mRNA 
0   0  3  9  10  NM_164448.1  CG4244-RA, transcript variant A (Su(dx)), mRNA 
0   0  3  9  10  NM_057405.2  CG4244-RB, transcript variant B (Su(dx)), mRNA 
0   0  3  3  8  NM_134544.1  CG11734-RB (HERC2), mRNA 
0   0  3  3  4  NM_135633.2  CG4636-RA (SCAR), mRNA 
0   0  3  3  3  NM_057965.3  CG3051-RA, transcript variant A (SNF1A), mRNA 
0   0  3  3  3  NM_166880.1  CG3051-RB, transcript variant B (SNF1A), mRNA 
0   0  3  3  3  NM_206604.1  CG3051-RC, transcript variant C (SNF1A), mRNA 
0   0  3  3  2  NM_137727.1  CG10505-RA (CG10505), mRNA 
0   0  3  2  14  NM_167735.1  CG32521-RB, transcript variant B (CG32521), mRNA 
0   0  3  2  14  NM_167734.1  CG32521-RA, transcript variant A (CG32521), mRNA 

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