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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7225R-1 |
| Symbol |
wbl |
Full Name |
windbeutel |
| CG No |
CG7225 |
Old CG No |
CG7225 |
| Synonyms |
wind, Windb, wbt, Wind, CG7225, wbl |
| Accession No (Link to NCBI) |
NM_166339.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTGGATGAGCTGAGTTTCGAG |
| Primer Seq. 3' |
aatctagaggtaccATCAGATAGGCCCTGGCGTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTNGNTGAGC TGAGTTTCGA GAAGACGGTG GAACGATCTT CCCTACTCCG TAGTGAAATT
0061 TGATATCGCA TATCCGTATG GGGAAAAGCA TGAGGCCTTT ACCGCCTTCT CCAAATCTGC
0121 CCACAAGGCA ACTAAAGATC TGCTAATNAG CCACCGTGGG TGTCNAAGGA CTATGGAGTA
0181 ACTGGAGAAC ANAGGCCCTG GGCGACCGTT ACAAAGTCGA CGACAAGAAC TTCCCGAGTA
0241 TCTTCCTGTT CAAGGGCAAC GCTGATGAGT ACGTCCAGCT TCCTAGNCAC GTCGACGTAA
0301 CGCTGGACAA TCTGAAGGCT TTTGTCAGTG CCAACACACC TCTGTACATC GGTCGTGATG
0361 GCTGCATCAA AGAGTTCAAC GAAGTGCTCA AGAACTATGC CAATATCCCC GATGCAGAGC
0421 AACTGAAGCT TATCGAAAAG CTGCAGGCCA AGCAGGAGCA GCTGACCGAT CCCGAGCAGC
0481 AGCAGAACgC CAGGGCCTAT CTGAT |
| in silico PCR Fragment |
0001 CTGGATGAGC TGAGTTTCGA GAAGACGGTG GAACGAT-TT CCCTACTCCG TAGTGAAATT
0061 TGATATCGCA TATCCGTATG GGGAAAAGCA TGAGGCCTTT ACCGCCTTCT CCAAATCTGC
0121 CCACAAGGCA ACTAAAGATC TGCTAAT-AG CCACCGTGGG TGTC-AAGGA CTATGGAG-A
0181 ACTGGAGAAC A-AGGCCCTG GGCGACCGTT ACAAAGTCGA CGACAAGAAC TTCCCGAGTA
0241 TCTTCCTGTT CAAGGGCAAC GCTGATGAGT ACGTCCAGCT TCCTAGCCAC GTCGACGTAA
0301 CGCTGGACAA TCTGAAGGCT TTTGTCAGTG CCAACACACC TCTGTACATC GGTCGTGATG
0361 GCTGCATCAA AGAGTTCAAC GAAGTGCTCA AGAACTATGC CAATATCCCC GATGCAGAGC
0421 AACTGAAGCT TATCGAAAAG CTGCAGGCCA AGCAGGAGCA GCTGACCGAT CCCGAGCAGC
0481 AGCAGAACGC CAGGGCCTAT CTGAT |
| Assemble Data |
7225R-1.IR_full 1 CTNGNTGAGCTGAGTTTCGAGAAGACGGTGGAACGATCTTCCCTACTCCGTAGTGAAATT 60
|| | |||||||||||||||||||||||||||||||| ||||||||||||||||||||||
7225R-1.in silico 1 CTGGATGAGCTGAGTTTCGAGAAGACGGTGGAACGAT-TTCCCTACTCCGTAGTGAAATT 60
7225R-1.IR_full 61 TGATATCGCATATCCGTATGGGGAAAAGCATGAGGCCTTTACCGCCTTCTCCAAATCTGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7225R-1.in silico 61 TGATATCGCATATCCGTATGGGGAAAAGCATGAGGCCTTTACCGCCTTCTCCAAATCTGC 120
7225R-1.IR_full 121 CCACAAGGCAACTAAAGATCTGCTAATNAGCCACCGTGGGTGTCNAAGGACTATGGAGTA 180
||||||||||||||||||||||||||| |||||||||||||||| ||||||||||||| |
7225R-1.in silico 121 CCACAAGGCAACTAAAGATCTGCTAAT-AGCCACCGTGGGTGTC-AAGGACTATGGAG-A 180
7225R-1.IR_full 181 ACTGGAGAACANAGGCCCTGGGCGACCGTTACAAAGTCGACGACAAGAACTTCCCGAGTA 240
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
7225R-1.in silico 181 ACTGGAGAACA-AGGCCCTGGGCGACCGTTACAAAGTCGACGACAAGAACTTCCCGAGTA 240
7225R-1.IR_full 241 TCTTCCTGTTCAAGGGCAACGCTGATGAGTACGTCCAGCTTCCTAGNCACGTCGACGTAA 300
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
7225R-1.in silico 241 TCTTCCTGTTCAAGGGCAACGCTGATGAGTACGTCCAGCTTCCTAGCCACGTCGACGTAA 300
7225R-1.IR_full 301 CGCTGGACAATCTGAAGGCTTTTGTCAGTGCCAACACACCTCTGTACATCGGTCGTGATG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7225R-1.in silico 301 CGCTGGACAATCTGAAGGCTTTTGTCAGTGCCAACACACCTCTGTACATCGGTCGTGATG 360
7225R-1.IR_full 361 GCTGCATCAAAGAGTTCAACGAAGTGCTCAAGAACTATGCCAATATCCCCGATGCAGAGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7225R-1.in silico 361 GCTGCATCAAAGAGTTCAACGAAGTGCTCAAGAACTATGCCAATATCCCCGATGCAGAGC 420
7225R-1.IR_full 421 AACTGAAGCTTATCGAAAAGCTGCAGGCCAAGCAGGAGCAGCTGACCGATCCCGAGCAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7225R-1.in silico 421 AACTGAAGCTTATCGAAAAGCTGCAGGCCAAGCAGGAGCAGCTGACCGATCCCGAGCAGC 480
7225R-1.IR_full 481 AGCAGAACGCCAGGGCCTATCTGAT 505
|||||||||||||||||||||||||
7225R-1.in silico 481 AGCAGAACGCCAGGGCCTATCTGAT 505
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
6 |
NM_166339.1 |
CG7225-RA (wbl), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_168064.2 |
CG14998-RA, transcript variant A (CG14998), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_168063.2 |
CG14998-RD, transcript variant D (CG14998), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_168062.2 |
CG14998-RE, transcript variant E (CG14998), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_168061.2 |
CG14998-RB, transcript variant B (CG14998), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_139604.2 |
CG14998-RC, transcript variant C (CG14998), mRNA |
| 0 |
0 |
5 |
2 |
4 |
NM_166929.1 |
CG3835-RB, transcript variant B (CG3835), mRNA |
| 0 |
0 |
5 |
2 |
4 |
NM_166930.1 |
CG3835-RC, transcript variant C (CG3835), mRNA |
| 0 |
0 |
5 |
2 |
4 |
NM_130626.2 |
CG3835-RA, transcript variant A (CG3835), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_169141.1 |
CG1021-RA, transcript variant A (CG1021), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_141397.2 |
CG1021-RB, transcript variant B (CG1021), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078993.2 |
CG8604-RA (Amph), mRNA |
| 0 |
0 |
2 |
13 |
17 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_137712.3 |
CG30389-RC, transcript variant C (CG30389), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_137711.3 |
CG30389-RA, transcript variant A (CG30389), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_166428.2 |
CG30389-RB, transcript variant B (CG30389), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_132956.1 |
CG9086-RA (CG9086), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_078523.2 |
CG2252-RB, transcript variant B (fs(1)h), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_167144.2 |
CG2252-RA, transcript variant A (fs(1)h), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_206647.1 |
CG2252-RC, transcript variant C (fs(1)h), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_206646.1 |
CG2252-RD, transcript variant D (fs(1)h), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_206645.1 |
CG2252-RE, transcript variant E (fs(1)h), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_137695.2 |
CG10540-RA (cpa), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141948.1 |
CG5333-RA (trus), mRNA |
| 0 |
0 |
1 |
10 |
9 |
NM_138220.1 |
CG13889-RA (CG13889), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_135101.1 |
CG18266-RA (CG18266), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_132636.2 |
CG12723-RA (CG12723), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_166250.1 |
CG30111-RA (CG30111), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141529.2 |
CG11694-RA (CG11694), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_143344.2 |
CG5520-RA (Gp93), mRNA |
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