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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7215R-1 |
| Symbol |
CG7215 |
Full Name |
CG7215 |
| CG No |
CG7215 |
Old CG No |
CG7215 |
| Synonyms |
CG32920, CG7215 |
| Accession No (Link to NCBI) |
NM_142423.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAACAATCAAGGTGCTGAAGGG |
| Primer Seq. 3' |
aatctagaggtaccTGTGGCCGCTCTGTTGACTAT |
| Predicted Fragment Size |
382 |
| IR fragment full Seq |
0001 AACAATCAAG GTGCTGAAGG GCAAGGACTG CACCATCGAG GTGGCGCCGA CATCGACGAT
0061 TCTGGAGGTG AAACACCAGA TCGAGGCGGA ACTGCAGATA TCGGCTACAA ATCAAAAGCT
0121 ACTGCTGTTG GGGCGACCGC TTAACAATGA ACAGACAATT GCGTCATATC CCAACATCAA
0181 GGAGGGCACC AAACTCAATC TGGTGGTGAT AAAGCCCTGT TTAAGGGACA GCATCCTGCG
0241 GGGATTCCGC AAGCACTATT CGGAGCCCCT AGCTGAGCGT ATGACCAATG AGTTTATGGC
0301 CGACTTCGAG CGGAAAATCA ACGAGCAGAG CCTGGACGAC CTGGAGAGAC TGGCGGACAG
0361 CATAGTCAAC AGAGCGGCCA CA |
| in silico PCR Fragment |
0001 AACAATCAAG GTGCTGAAGG GCAAGGACTG CACCATCGAG GTGGCGCCGA CATCGACGAT
0061 TCTGGAGGTG AAACACCAGA TCGAGGCGGA ACTGCAGATA TCGGCTACAA ATCAAAAGCT
0121 ACTGCTGTTG GGGCGACCGC TTAACAATGA ACAGACAATT GCGTCATATC CCAACATCAA
0181 GGAGGGCACC AAACTCAATC TGGTGGTGAT AAAGCCCTGT TTAAGGGACA GCATCCTGCG
0241 GGGATTCCGC AAGCACTATT CGGAGCCCCT AGCTGAGCGT ATGACCAATG AGTTTATGGC
0301 CGACTTCGAG CGGAAAATCA ACGAGCAGAG CCTGGACGAC CTGGAGAGAC TGGCGGACAG
0361 CATAGTCAAC AGAGCGGCCA CA |
| Assemble Data |
7215R-1.IR_full 1 AACAATCAAGGTGCTGAAGGGCAAGGACTGCACCATCGAGGTGGCGCCGACATCGACGAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 1 AACAATCAAGGTGCTGAAGGGCAAGGACTGCACCATCGAGGTGGCGCCGACATCGACGAT 60
7215R-1.IR_full 61 TCTGGAGGTGAAACACCAGATCGAGGCGGAACTGCAGATATCGGCTACAAATCAAAAGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 61 TCTGGAGGTGAAACACCAGATCGAGGCGGAACTGCAGATATCGGCTACAAATCAAAAGCT 120
7215R-1.IR_full 121 ACTGCTGTTGGGGCGACCGCTTAACAATGAACAGACAATTGCGTCATATCCCAACATCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 121 ACTGCTGTTGGGGCGACCGCTTAACAATGAACAGACAATTGCGTCATATCCCAACATCAA 180
7215R-1.IR_full 181 GGAGGGCACCAAACTCAATCTGGTGGTGATAAAGCCCTGTTTAAGGGACAGCATCCTGCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 181 GGAGGGCACCAAACTCAATCTGGTGGTGATAAAGCCCTGTTTAAGGGACAGCATCCTGCG 240
7215R-1.IR_full 241 GGGATTCCGCAAGCACTATTCGGAGCCCCTAGCTGAGCGTATGACCAATGAGTTTATGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 241 GGGATTCCGCAAGCACTATTCGGAGCCCCTAGCTGAGCGTATGACCAATGAGTTTATGGC 300
7215R-1.IR_full 301 CGACTTCGAGCGGAAAATCAACGAGCAGAGCCTGGACGACCTGGAGAGACTGGCGGACAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7215R-1.in silico 301 CGACTTCGAGCGGAAAATCAACGAGCAGAGCCTGGACGACCTGGAGAGACTGGCGGACAG 360
7215R-1.IR_full 361 CATAGTCAACAGAGCGGCCACA 382
||||||||||||||||||||||
7215R-1.in silico 361 CATAGTCAACAGAGCGGCCACA 382
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
364 |
0 |
0 |
0 |
NM_142423.2 |
CG7215-RA, transcript variant A (CG7215), mRNA |
| 100 |
364 |
0 |
0 |
0 |
NM_142422.3 |
CG7217-RA, transcript variant A (CG7217), mRNA |
| 0 |
0 |
7 |
3 |
2 |
NM_135839.2 |
CG7953-RA (CG7953), mRNA |
| 0 |
0 |
5 |
3 |
4 |
NM_141078.1 |
CG14574-RA (CG14574), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_205912.1 |
CG11020-RB, transcript variant B (nompC), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_078759.2 |
CG11020-RA, transcript variant A (nompC), mRNA |
| 0 |
0 |
5 |
2 |
4 |
NM_164858.1 |
CG4405-RB, transcript variant B (jp), mRNA |
| 0 |
0 |
5 |
2 |
4 |
NM_078801.4 |
CG4405-RA, transcript variant A (jp), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_143033.2 |
CG5807-RA (CG5807), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079739.2 |
CG10161-RB (eIF-3p66), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135077.2 |
CG14023-RA (CG14023), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_142081.2 |
CG3172-RA (twf), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_079491.2 |
CG5723-RB (Ten-m), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_079903.2 |
CG15319-RB (nej), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_080140.2 |
CG3923-RA (Exp6), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135797.2 |
CG6523-RA (CG6523), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_130619.2 |
CG4194-RA (CG4194), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_079996.2 |
CG18024-RA (SoxN), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_137815.2 |
CG3380-RA (Oatp58Dc), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_141401.1 |
CG2330-RA (CG2330), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_205948.1 |
CG15828-RB, transcript variant B (CG15828), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135460.1 |
CG15828-RA, transcript variant A (CG15828), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132320.2 |
CG12124-RA (CG12124), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136877.3 |
CG8357-RA (Rep1), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_144350.1 |
CG7487-RA (RecQ4), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_176680.2 |
CG33082-RA (CG33082), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135437.3 |
CG3748-RB, transcript variant B (CG3748), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_143728.2 |
CG14630-RA (CG14630), mRNA |
| 0 |
0 |
0 |
3 |
12 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_132041.2 |
CG15765-RA (CG15765), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
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| VB |
 |
| |
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