NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7214R-4

Symbol CG7214 Full Name CG7214

CG No CG7214 Old CG No CG7214

Synonyms CG7214

Accession No (Link to NCBI) NM_135297.2

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAAGTTCGCCGTTGCCGTAGT

Primer Seq. 3'

aatctagaggtaccACGTTCACGGAGGTGGCAGAG

Predicted Fragment Size

IR fragment full Seq

0001 GAAGTTCGCC GTTGCCGTAG TCATGTTCGT GTGCGCCCTG GCTGGAGCTC ATGCCTCGTG 
0061 GGCCCAGATC GCTCCCGGTG TGTCCTATGT GAGCCCCGTG GCCCATGGAG CCGGTCTGTG 
0121 GAACGGAGCC TGGAATGGTG CCTGGAACGG TGCCTGGAAC GGCGCCTGGA ACGGTGCTCC 
0181 CTGGGGTGCC CCTGCCGCCG TTGCCGTCCA TGCCAGCGCT GCTCCCTGGG GTCTGACCGG 
0241 TGCCGGATGG CACNGNGCTG CTGTTCCCGG AATCAACCTG GCCCAGGGAC CCGGTCTCGC 
0301 CGCCgGCCAT GGCCATGAGG GCGTCTATGT GGCCAAGACC CGTGGTGCCA TCCACACCGC 
0361 TCCCCTCGCC GGACACATCA ACTCTGCCAC CTCCGTGAAC GT

in silico PCR Fragment

0001 GAAGTTCGCC GTTGCCGTAG TCATGTTCGT GTGCGCCCTG GCTGGAGCTC ATGCCTCGTG 
0061 GGCCCAGATC GCTCCCGGTG TGTCCTATGT GAGCCCCGTG GCCCATGGAG CCGGTCTGTG 
0121 GAACGGAGCC TGGAATGGTG CCTGGAACGG TGCCTGGAAC GGCGCCTGGA ACGGTGCTCC 
0181 CTGGGGTGCC CCTGCCGCCG TTGCCGTCCA TGCCAGCGCT GCTCCCTGGG GTCTGACCGG 
0241 TGCCGGATGG CACGGCGCTG CTGTTCCCGG AATCAACCTG GCCCAGGGAC CCGGTCTCGC 
0301 CGCCGGCCAT GGCCATGAGG GCGTCTATGT GGCCAAGACC CGTGGTGCCA TCCACACCGC 
0361 TCCCCTCGCC GGACACATCA ACTCTGCCAC CTCCGTGAAC GT

Assemble Data


7214R-4.IR_full       1   GAAGTTCGCCGTTGCCGTAGTCATGTTCGTGTGCGCCCTGGCTGGAGCTCATGCCTCGTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     1   GAAGTTCGCCGTTGCCGTAGTCATGTTCGTGTGCGCCCTGGCTGGAGCTCATGCCTCGTG 60


7214R-4.IR_full       61  GGCCCAGATCGCTCCCGGTGTGTCCTATGTGAGCCCCGTGGCCCATGGAGCCGGTCTGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     61  GGCCCAGATCGCTCCCGGTGTGTCCTATGTGAGCCCCGTGGCCCATGGAGCCGGTCTGTG 120


7214R-4.IR_full       121 GAACGGAGCCTGGAATGGTGCCTGGAACGGTGCCTGGAACGGCGCCTGGAACGGTGCTCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     121 GAACGGAGCCTGGAATGGTGCCTGGAACGGTGCCTGGAACGGCGCCTGGAACGGTGCTCC 180


7214R-4.IR_full       181 CTGGGGTGCCCCTGCCGCCGTTGCCGTCCATGCCAGCGCTGCTCCCTGGGGTCTGACCGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     181 CTGGGGTGCCCCTGCCGCCGTTGCCGTCCATGCCAGCGCTGCTCCCTGGGGTCTGACCGG 240


7214R-4.IR_full       241 TGCCGGATGGCACNGNGCTGCTGTTCCCGGAATCAACCTGGCCCAGGGACCCGGTCTCGC 300
                          ||||||||||||| | ||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     241 TGCCGGATGGCACGGCGCTGCTGTTCCCGGAATCAACCTGGCCCAGGGACCCGGTCTCGC 300


7214R-4.IR_full       301 CGCCGGCCATGGCCATGAGGGCGTCTATGTGGCCAAGACCCGTGGTGCCATCCACACCGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     301 CGCCGGCCATGGCCATGAGGGCGTCTATGTGGCCAAGACCCGTGGTGCCATCCACACCGC 360


7214R-4.IR_full       361 TCCCCTCGCCGGACACATCAACTCTGCCACCTCCGTGAACGT 402
                          ||||||||||||||||||||||||||||||||||||||||||
7214R-4.in silico     361 TCCCCTCGCCGGACACATCAACTCTGCCACCTCCGTGAACGT 402

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	384	26	52	52	NM_135297.2	CG7214-RA (CG7214), mRNA
0.78	3	8	10	11	NM_135299.2	CG7203-RA (CG7203), mRNA
0.26	1	3	5	6	NM_140616.1	CG13060-RA (CG13060), mRNA
0	0	12	15	11	NM_057767.3	CG7216-RA (Acp1), mRNA
0	0	12	15	6	NM_164768.1	CG31904-RB, transcript variant B (CG31904), mRNA
0	0	12	15	6	NM_164769.1	CG31904-RD, transcript variant D (CG31904), mRNA
0	0	2	5	10	NM_141198.3	CG9776-RA, transcript variant A (CG9776), mRNA
0	0	2	2	5	NM_135439.1	CG13111-RA (CG13111), mRNA
0	0	2	2	3	NM_142679.1	CG7079-RA (CG7079), mRNA
0	0	1	7	8	NM_140615.1	CG13041-RA (CG13041), mRNA
0	0	1	6	4	NM_206250.2	CG16973-RC, transcript variant C (msn), mRNA
0	0	1	6	4	NM_079940.4	CG16973-RA, transcript variant A (msn), mRNA
0	0	1	6	4	NM_206252.2	CG16973-RB, transcript variant B (msn), mRNA
0	0	1	6	4	NM_206251.2	CG16973-RD, transcript variant D (msn), mRNA
0	0	1	6	3	NM_206249.2	CG16973-RE, transcript variant E (msn), mRNA
0	0	1	5	8	NM_143316.2	CG5586-RB (Tusp), mRNA
0	0	1	4	10	NM_057458.3	CG12749-RB, transcript variant B (Hrb87F), mRNA
0	0	1	4	10	NM_057459.3	CG12749-RA, transcript variant A (Hrb87F), mRNA
0	0	1	4	8	NM_140082.1	CG8329-RA (CG8329), mRNA
0	0	1	4	6	NM_137713.2	CG4266-RA (CG4266), mRNA
0	0	1	4	3	NM_142924.2	CG10219-RA (CG10219), mRNA
0	0	1	3	3	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	1	3	3	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	1	3	3	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	1	3	3	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	0	10	10	NM_132709.1	CG12480-RA (CG12480), mRNA
0	0	0	6	6	NM_135169.2	CG9493-RA (Pez), mRNA
0	0	0	5	9	NM_079647.2	CG8884-RF, transcript variant F (Sap47), mRNA
0	0	0	5	9	NM_169690.1	CG8884-RB, transcript variant B (Sap47), mRNA
0	0	0	5	9	NM_169685.1	CG8884-RC, transcript variant C (Sap47), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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