NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7091R-3

Symbol

CG7091

Full Name

CG7091

CG No

CG7091

Old CG No

CG7091

Synonyms

CG7091

Accession No (Link to NCBI)

NM_141986.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

male pupal semi-lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCATGTCATGGACCCTGCCAC

Primer Seq. 3'

aatctagaggtaccATCAACCACTCCGGCCTCGAA

Predicted Fragment Size

IR fragment full Seq

0001 GCATGTCATG GACCCTGCCA CGCCTCTCGC AGTCCAGTGC ATCCACCGCC CACGGACTAG 
0061 TGGGTTCCGT TCGCCTGACC TACGCCATAT GTGCCTTTTT GGCAACTTGC CTACACGCCG 
0121 CCATGAGGAA CATGCTCGGC ATGATCATCC TCAAGATGGT CATGCCCCGG CCGGAGGATG 
0181 CACTGGTCGT TCCAGCGGGG TTGAGTCGCC TAACGGAGGG CAATGTCACC TCCACGGGTC 
0241 GATGTGGATC TCCTCGGGTC GTTTTCAATC CGCAGATTCA AGAGACTCAG TCGGGCGACT 
0301 TACCATGGAC CCGAAACCAG GAGCTGACCT TCCCAGGTGT CTACTACTAT GGCTACGTGG 
0361 TTTCCATCTC GCTATCCGGC TATCTGGCAG ATCGGTGCAG CAGCAAGCGG CTGTTCATCG 
0421 TGTCCCTGAT CTTCGAGGCT GTGGCCTACA TACTGCTCCC AGCAATGGCG CACTCTAGTT 
0481 TCGAGGCCGG AGTGGTTGAT

in silico PCR Fragment

0001 GCATGTCATG GACCCTGCCA CGCCTCTCGC AGTCCAGTGC ATCCACCGCC CACGGACTAG 
0061 TGGGTTCCGT TCGCCTGACC TACGCCATAT GTGCCTTTTT GGCAACTTGC CTACACGCCG 
0121 CCATGAGGAA CATGCTCGGC ATGATCATCC TCAAGATGGT CATGCCCCGG CCGGAGGATG 
0181 CACTGGTCGT TCCAGCGGGG TTGAGTCGCC TAACGGAGGG CAATGTCACC TCCACGGGTC 
0241 GATGTGGATC TCCTCGGGTC GTTTTCAATC CGCAGATTCA AGAGACTCAG TCGGGCGACT 
0301 TACCATGGAC CCGAAACCAG GAGCTGACCT TCCCAGGTGT CTACTACTAT GGCTACGTGG 
0361 TTTCCATCTC GCTATCCGGC TATCTGGCAG ATCGGTGCAG CAGCAAGCGG CTGTTCATCG 
0421 TGTCCCTGAT CTTCGAGGCT GTGGCCTACA TACTGCTCCC AGCAATGGCG CACTCTAGTT 
0481 TCGAGGCCGG AGTGGTTGAT

Assemble Data


7091R-3.IR_full       1   GCATGTCATGGACCCTGCCACGCCTCTCGCAGTCCAGTGCATCCACCGCCCACGGACTAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     1   GCATGTCATGGACCCTGCCACGCCTCTCGCAGTCCAGTGCATCCACCGCCCACGGACTAG 60


7091R-3.IR_full       61  TGGGTTCCGTTCGCCTGACCTACGCCATATGTGCCTTTTTGGCAACTTGCCTACACGCCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     61  TGGGTTCCGTTCGCCTGACCTACGCCATATGTGCCTTTTTGGCAACTTGCCTACACGCCG 120


7091R-3.IR_full       121 CCATGAGGAACATGCTCGGCATGATCATCCTCAAGATGGTCATGCCCCGGCCGGAGGATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     121 CCATGAGGAACATGCTCGGCATGATCATCCTCAAGATGGTCATGCCCCGGCCGGAGGATG 180


7091R-3.IR_full       181 CACTGGTCGTTCCAGCGGGGTTGAGTCGCCTAACGGAGGGCAATGTCACCTCCACGGGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     181 CACTGGTCGTTCCAGCGGGGTTGAGTCGCCTAACGGAGGGCAATGTCACCTCCACGGGTC 240


7091R-3.IR_full       241 GATGTGGATCTCCTCGGGTCGTTTTCAATCCGCAGATTCAAGAGACTCAGTCGGGCGACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     241 GATGTGGATCTCCTCGGGTCGTTTTCAATCCGCAGATTCAAGAGACTCAGTCGGGCGACT 300


7091R-3.IR_full       301 TACCATGGACCCGAAACCAGGAGCTGACCTTCCCAGGTGTCTACTACTATGGCTACGTGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     301 TACCATGGACCCGAAACCAGGAGCTGACCTTCCCAGGTGTCTACTACTATGGCTACGTGG 360


7091R-3.IR_full       361 TTTCCATCTCGCTATCCGGCTATCTGGCAGATCGGTGCAGCAGCAAGCGGCTGTTCATCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     361 TTTCCATCTCGCTATCCGGCTATCTGGCAGATCGGTGCAGCAGCAAGCGGCTGTTCATCG 420


7091R-3.IR_full       421 TGTCCCTGATCTTCGAGGCTGTGGCCTACATACTGCTCCCAGCAATGGCGCACTCTAGTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7091R-3.in silico     421 TGTCCCTGATCTTCGAGGCTGTGGCCTACATACTGCTCCCAGCAATGGCGCACTCTAGTT 480


7091R-3.IR_full       481 TCGAGGCCGGAGTGGTTGAT 500
                          ||||||||||||||||||||
7091R-3.in silico     481 TCGAGGCCGGAGTGGTTGAT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	6	NM_141986.2	CG7091-RA (CG7091), mRNA
0.62	3	2	3	2	NM_080055.2	CG3895-RA (ph-d), mRNA
0.62	3	2	3	2	NM_057523.3	CG18412-RA (ph-p), mRNA
0.2	1	2	2	10	NM_057411.3	CG10079-RB, transcript variant B (Egfr), mRNA
0.2	1	2	2	10	NM_057410.3	CG10079-RA, transcript variant A (Egfr), mRNA
0	0	3	4	2	NM_140665.1	CG9674-RA, transcript variant A (CG9674), mRNA
0	0	3	4	2	NM_176339.1	CG9674-RD, transcript variant D (CG9674), mRNA
0	0	3	2	2	NM_135785.1	CG6043-RD, transcript variant D (CG6043), mRNA
0	0	3	2	2	NM_165035.1	CG6043-RB, transcript variant B (CG6043), mRNA
0	0	3	2	2	NM_165037.1	CG6043-RC, transcript variant C (CG6043), mRNA
0	0	3	2	2	NM_165034.1	CG6043-RA, transcript variant A (CG6043), mRNA
0	0	1	3	5	NM_170336.1	CG5643-RA, transcript variant A (wdb), mRNA
0	0	1	3	5	NM_143312.2	CG5643-RC, transcript variant C (wdb), mRNA
0	0	1	3	5	NM_170339.1	CG5643-RE, transcript variant E (wdb), mRNA
0	0	1	3	5	NM_170341.1	CG5643-RG, transcript variant G (wdb), mRNA
0	0	1	3	5	NM_170338.1	CG5643-RD, transcript variant D (wdb), mRNA
0	0	1	3	5	NM_170337.1	CG5643-RB, transcript variant B (wdb), mRNA
0	0	1	3	5	NM_170340.1	CG5643-RF, transcript variant F (wdb), mRNA
0	0	1	3	5	NM_168357.2	CG32045-RB, transcript variant B (fry), mRNA
0	0	1	3	5	NM_001043133.1	CG32045-RD, transcript variant D (fry), mRNA
0	0	1	3	4	NM_166100.1	CG30471-RA (CG30471), mRNA
0	0	1	3	4	NM_137498.1	CG15069-RA, transcript variant A (Rgk2), mRNA
0	0	1	3	3	NM_137192.1	CG12958-RA (CG12958), mRNA
0	0	1	3	3	NM_166295.1	CG15069-RB, transcript variant B (Rgk2), mRNA
0	0	1	3	2	NM_137327.2	CG6665-RA, transcript variant A (CG6665), mRNA
0	0	1	3	2	NM_166204.1	CG6665-RC, transcript variant C (CG6665), mRNA
0	0	1	2	6	NM_140314.2	CG10698-RA (GRHRII), mRNA
0	0	1	2	4	NM_141669.2	CG8362-RA (nmdyn-D7), mRNA
0	0	1	2	2	NM_142050.2	CG9347-RA (ninaB), mRNA
0	0	0	6	3	NM_139501.2	CG2114-RA (FR), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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