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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7082R-1 |
| Symbol |
CG7082 |
Full Name |
CG7082 |
| CG No |
CG7082 |
Old CG No |
CG7082 |
| Synonyms |
cg7082, CG7082 |
| Accession No (Link to NCBI) |
NM_164461.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTACTTCTGGGCTTCGGACT |
| Primer Seq. 3' |
aatctagaggtaccCCTTTCCATTCCGCCCATTTA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACTACTTCTG GGCTTCGGAC TATGCTCCCT TggGGGCGCC ATGCTATATG CCTATTTCAA
0061 GACTCGAAAC GACGAGGAGG AAGCGGACTC AGGTGGCCAA CGGNCNGCAT CCGGGATTAG
0121 AGGACAGACA GAGGAACAGA AGCCCCAGAA GGAAGTGTGC CTCAAAATCG TCGTGGACAA
0181 CGAGCATGTG CCCCTGATAA TGGGTCGCGG TGGATCCAAT ATCAAGTTGA TTGAAGAAAA
0241 GACCCTGGCC AAGATAAGAT TACGAGACAA AGACAGTGGC CACAAATTCT GCGACATTAG
0301 CGGCGTTCCC GATGCGGTTA AGGCGGCTCG AgCTCTGCTT ATCAAGGAAA TTGAACGTGC
0361 GCCCGTGGTG AAAGTGGAAC TGCAGGTGCC CCAGAGGCTG GCCAGCAAAA TTAACGGACG
0421 CGGCGGCGAG CTCCTCCAGG AGATCCGGAG CAGTTCGCTA gCCAAGCTGA ATATCGATTT
0481 AAATGGGCGG AATGGAAAGG |
| in silico PCR Fragment |
0001 ACTACTTCTG GGCTTCGGAC TATGCTCCCT TGGGGGCGCC ATGCTATATG CCTATTTCAA
0061 GACTCGAAAC GACGAGGAGG AAGCGGACTC AGGTGGCCAA CGGCCGGCAT CCGGGATTAG
0121 AGGACAGACA GAGGAACAGA AGCCCCAGAA GGAAGTGTGC CTCAAAATCG TCGTGGACAA
0181 CGAGCATGTG CCCCTGATAA TGGGTCGCGG TGGATCCAAT ATCAAGTTGA TTGAAGAAAA
0241 GACCCTGGCC AAGATAAGAT TACGAGACAA AGACAGTGGC CACAAATTCT GCGACATTAG
0301 CGGCGTTCCC GATGCGGTTA AGGCGGCTCG AGCTCTGCTT ATCAAGGAAA TTGAACGTGC
0361 GCCCGTGGTG AAAGTGGAAC TGCAGGTGCC CCAGAGGCTG GCCAGCAAAA TTAACGGACG
0421 CGGCGGCGAG CTCCTCCAGG AGATCCGGAG CAGTTCGCTA GCCAAGCTGA ATATCGATTT
0481 AAATGGGCGG AATGGAAAGG |
| Assemble Data |
7082R-1.IR_full 1 ACTACTTCTGGGCTTCGGACTATGCTCCCTTGGGGGCGCCATGCTATATGCCTATTTCAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 1 ACTACTTCTGGGCTTCGGACTATGCTCCCTTGGGGGCGCCATGCTATATGCCTATTTCAA 60
7082R-1.IR_full 61 GACTCGAAACGACGAGGAGGAAGCGGACTCAGGTGGCCAACGGNCNGCATCCGGGATTAG 120
||||||||||||||||||||||||||||||||||||||||||| | ||||||||||||||
7082R-1.in silico 61 GACTCGAAACGACGAGGAGGAAGCGGACTCAGGTGGCCAACGGCCGGCATCCGGGATTAG 120
7082R-1.IR_full 121 AGGACAGACAGAGGAACAGAAGCCCCAGAAGGAAGTGTGCCTCAAAATCGTCGTGGACAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 121 AGGACAGACAGAGGAACAGAAGCCCCAGAAGGAAGTGTGCCTCAAAATCGTCGTGGACAA 180
7082R-1.IR_full 181 CGAGCATGTGCCCCTGATAATGGGTCGCGGTGGATCCAATATCAAGTTGATTGAAGAAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 181 CGAGCATGTGCCCCTGATAATGGGTCGCGGTGGATCCAATATCAAGTTGATTGAAGAAAA 240
7082R-1.IR_full 241 GACCCTGGCCAAGATAAGATTACGAGACAAAGACAGTGGCCACAAATTCTGCGACATTAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 241 GACCCTGGCCAAGATAAGATTACGAGACAAAGACAGTGGCCACAAATTCTGCGACATTAG 300
7082R-1.IR_full 301 CGGCGTTCCCGATGCGGTTAAGGCGGCTCGAGCTCTGCTTATCAAGGAAATTGAACGTGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 301 CGGCGTTCCCGATGCGGTTAAGGCGGCTCGAGCTCTGCTTATCAAGGAAATTGAACGTGC 360
7082R-1.IR_full 361 GCCCGTGGTGAAAGTGGAACTGCAGGTGCCCCAGAGGCTGGCCAGCAAAATTAACGGACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 361 GCCCGTGGTGAAAGTGGAACTGCAGGTGCCCCAGAGGCTGGCCAGCAAAATTAACGGACG 420
7082R-1.IR_full 421 CGGCGGCGAGCTCCTCCAGGAGATCCGGAGCAGTTCGCTAGCCAAGCTGAATATCGATTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7082R-1.in silico 421 CGGCGGCGAGCTCCTCCAGGAGATCCGGAGCAGTTCGCTAGCCAAGCTGAATATCGATTT 480
7082R-1.IR_full 481 AAATGGGCGGAATGGAAAGG 500
||||||||||||||||||||
7082R-1.in silico 481 AAATGGGCGGAATGGAAAGG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_164461.1 |
CG7082-RA, transcript variant A (CG7082), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_134813.2 |
CG7082-RC, transcript variant C (CG7082), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_164462.1 |
CG7082-RB, transcript variant B (CG7082), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_164463.1 |
CG7082-RD, transcript variant D (CG7082), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_136489.2 |
CG8728-RA (CG8728), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132542.2 |
CG2471-RA (CG2471), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132447.1 |
CG11160-RA, transcript variant A (CG11160), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167262.1 |
CG11160-RB, transcript variant B (CG11160), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135841.4 |
CG8954-RA, transcript variant A (Smg5), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165069.1 |
CG8954-RB, transcript variant B (Smg5), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165920.1 |
CG17759-RB, transcript variant B (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078994.2 |
CG17759-RH, transcript variant H (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165922.1 |
CG17759-RE, transcript variant E (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165921.1 |
CG17759-RC, transcript variant C (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165923.1 |
CG17759-RG, transcript variant G (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165919.1 |
CG17759-RA, transcript variant A (Galpha49B), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NR_001637.1 |
CG17759-RA, transcript variant A (Galpha49B), mRNA, miscRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136815.1 |
CG13222-RA (CG13222), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132267.4 |
CG12109-RB (Caf1-180), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140908.1 |
CG7570-RA (hale), mRNA |
| 0 |
0 |
0 |
7 |
9 |
NM_132066.1 |
CG4320-RA (raptor), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_079729.2 |
CG4792-RA (Dcr-1), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_136398.3 |
CG9446-RA, transcript variant A (coro), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_206031.1 |
CG9446-RC, transcript variant C (coro), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_165494.1 |
CG9446-RB, transcript variant B (coro), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_132259.2 |
CG11219-RA (PIP82), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_166058.1 |
CG30197-RA (CG30197), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_130502.2 |
CG12311-RA (Pomt2), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_136812.1 |
CG13223-RA (CG13223), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NR_001327.1 |
CG13223-RA (CG13223), mRNA, mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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