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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7074R-3 |
| Symbol |
mio |
Full Name |
missing oocyte |
| CG No |
CG7074 |
Old CG No |
CG7074 |
| Synonyms |
CG15384, CG7074, mio |
| Accession No (Link to NCBI) |
NM_134812.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACGGACTCAGCTGGTTTCCAC |
| Primer Seq. 3' |
aatctagaggtaccCTGGCTCATTCCCGCAATGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACGNACTCAG CTGGTTTCCA CATTTTCCCG ACAAATTCGT ATCCTGGGGC CAGGAAATCC
0061 ATTTATACGA AGtGCGCCGC AAAGATGATC ACAGCCAAAA AAGTCGCCTG CCTTATATAN
0121 TCCGTGAACT ACCTCGCCAA CGAGTCGCGC TATCAATATG NGCCCGCTGC GTGGCCGCCT
0181 CCTACCACAG TGACCAGCCC CATAATTGCA GTGGGTCTGG CGGATGGCAA AGTCGGGATC
0241 TGCAACTTCC GGGACACCTA CGACAGCAGC TGGGAGTACA CACCGCGTCA GCANGGCGCA
0301 TGTGCACCTG CCTCGCCTGG AACGAGCTAG ATGCCAATAT CCTGGCCATT GGACACGATC
0361 GCCATCGAAA TGACACCTGC ATCACCATCT GGGACATTGA GCGTGGAGTT CCCAAGGAGA
0421 CGGCCAATTT TTTTGGCGTT GGCGAGTCGG CCAACTCCAT TTGCTGGGAT CGAAATCATC
0481 GCACGGTCAT TTGCGGGAAT GAGCCAG |
| in silico PCR Fragment |
0001 ACGGACTCAG CTGGTTTCCA CATTTTCCCG ACAAATTCGT ATCCTGGGGC CAGGAAATCC
0061 ATTTATACGA AGTGCGCCGC AAAGATGATC ACAGCCAAAA AAGTCGCCTG CCTTATATA-
0121 TCCGTGAACT ACCTCGCCAA CGAGTCGCGC TATCAATAT- -GCCCGCTGC GTGGCCGCCT
0181 CCTACCACAG TGACCAG-CC CATAATTGCA GTGGGTCTGG CGGATGGCAA AGTCGGGATT
0241 TGCAACTTCC GGGACACCTA CGACAGCAGC TGGGAGTACA CACCGCGTCA GCA--GCGCA
0301 TGTGCACCTG CCTCGCCTGG AACGAGCTGG ATGCCAATAT CCTGGCCATT GGACACGATC
0361 GCCATCGAAA TGACACCTGC ATCACCATCT GGGACATTGA GCGTGGAGTT CCCAAGGAGA
0421 CGGCCAACTT CTTCGGCGTC GGCGAGTCGG CCAACTCCAT TTGCTGGGAT CGAAATCATC
0481 GCACGGTCA- TTGCGGGAAT GAGCCAG |
| Assemble Data |
7074R-3.IR_full 1 ACGNACTCAGCTGGTTTCCACATTTTCCCGACAAATTCGTATCCTGGGGCCAGGAAATCC 60
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7074R-3.in silico 1 ACGGACTCAGCTGGTTTCCACATTTTCCCGACAAATTCGTATCCTGGGGCCAGGAAATCC 60
7074R-3.IR_full 61 ATTTATACGAAGTGCGCCGCAAAGATGATCACAGCCAAAAAAGTCGCCTGCCTTATATAN 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7074R-3.in silico 61 ATTTATACGAAGTGCGCCGCAAAGATGATCACAGCCAAAAAAGTCGCCTGCCTTATATA- 120
7074R-3.IR_full 121 TCCGTGAACTACCTCGCCAACGAGTCGCGCTATCAATATGNGCCCGCTGCGTGGCCGCCT 180
||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
7074R-3.in silico 121 TCCGTGAACTACCTCGCCAACGAGTCGCGCTATCAATAT--GCCCGCTGCGTGGCCGCCT 180
7074R-3.IR_full 181 CCTACCACAGTGACCAGCCCCATAATTGCAGTGGGTCTGGCGGATGGCAAAGTCGGGATC 240
||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
7074R-3.in silico 181 CCTACCACAGTGACCAG-CCCATAATTGCAGTGGGTCTGGCGGATGGCAAAGTCGGGATT 240
7074R-3.IR_full 241 TGCAACTTCCGGGACACCTACGACAGCAGCTGGGAGTACACACCGCGTCAGCANGGCGCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
7074R-3.in silico 241 TGCAACTTCCGGGACACCTACGACAGCAGCTGGGAGTACACACCGCGTCAGCA--GCGCA 300
7074R-3.IR_full 301 TGTGCACCTGCCTCGCCTGGAACGAGCTAGATGCCAATATCCTGGCCATTGGACACGATC 360
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
7074R-3.in silico 301 TGTGCACCTGCCTCGCCTGGAACGAGCTGGATGCCAATATCCTGGCCATTGGACACGATC 360
7074R-3.IR_full 361 GCCATCGAAATGACACCTGCATCACCATCTGGGACATTGAGCGTGGAGTTCCCAAGGAGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7074R-3.in silico 361 GCCATCGAAATGACACCTGCATCACCATCTGGGACATTGAGCGTGGAGTTCCCAAGGAGA 420
7074R-3.IR_full 421 CGGCCAATTTTTTTGGCGTTGGCGAGTCGGCCAACTCCATTTGCTGGGATCGAAATCATC 480
||||||| || || ||||| ||||||||||||||||||||||||||||||||||||||||
7074R-3.in silico 421 CGGCCAACTTCTTCGGCGTCGGCGAGTCGGCCAACTCCATTTGCTGGGATCGAAATCATC 480
7074R-3.IR_full 481 GCACGGTCATTTGCGGGAATGAGCCAG 507
||||||||| |||||||||||||||||
7074R-3.in silico 481 GCACGGTCA-TTGCGGGAATGAGCCAG 507
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_134812.2 |
CG7074-RA (mio), mRNA |
| 0.2 |
1 |
2 |
2 |
6 |
NM_079108.2 |
CG30170-RA (bgcn), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_167123.2 |
CG9650-RA, transcript variant A (CG9650), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_132157.1 |
CG9650-RB, transcript variant B (CG9650), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_167121.1 |
CG9650-RC, transcript variant C (CG9650), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079444.2 |
CG8727-RA (cyc), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_136669.2 |
CG10459-RA (CG10459), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_168023.1 |
CG11526-RA, transcript variant A (CG11526), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_133134.2 |
CG12359-RA (Ulp1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_130606.2 |
CG3573-RA (CG3573), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167544.1 |
CG9623-RA, transcript variant A (if), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078654.2 |
CG9623-RB, transcript variant B (if), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143745.2 |
CG18389-RA (Eip93F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136634.2 |
CG1975-RA (Rep2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132896.1 |
CG9981-RA (CG9981), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057263.2 |
CG2507-RA, transcript variant A (sas), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_132484.2 |
CG11750-RA (CG11750), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_140278.1 |
CG6938-RA (CG6938), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_130639.2 |
CG2918-RA (CG2918), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165341.1 |
CG1768-RB, transcript variant B (dia), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_057633.3 |
CG1768-RA, transcript variant A (dia), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_130662.2 |
CG2662-RA (CG2662), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_134897.2 |
CG17257-RB, transcript variant B (CG17257), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164522.1 |
CG17257-RA, transcript variant A (CG17257), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_166137.1 |
CG8405-RA (CG8405), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_176751.1 |
CG8557-RB, transcript variant B (CG8557), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166516.1 |
CG11170-RB (CG11170), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_139619.1 |
CG1332-RA (CG1332), mRNA |
| 0 |
0 |
0 |
3 |
13 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
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