NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7066R-1

Symbol

CG7066

Full Name

CG7066

CG No

CG7066

Old CG No

CG7066

Synonyms

CG7066

Accession No (Link to NCBI)

NM_139947.2

Inserted Chr.

Insertional Mutation

semi-lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATAAGGAGGAGCAGGAATCAG

Primer Seq. 3'

aatctagaggtaccGGTGCAGTTGTCACAATAGCT

Predicted Fragment Size

IR fragment full Seq

0001 ATAAGGAGGA GCAGGAATCA GGATGTCCAG GATACAGCGA TCAAGATTAC TCCACGCAgc 
0061 TCCAAGTACA AAAACCAGCA CAGGAAACGG GAGCAGCAAA CCTCTCTTCT GGACTTTGTC 
0121 ATCAAGCCGA GACCAAAAAC ACAAAGGCAA ACAAAGGCAC ATAAACTTCA AAAGACACAT 
0181 TTAGCCATAA CAAGAGGTAG CTACATAGTT TACAAACCCA AGGGCAAAAC CCGCCTTGAT 
0241 CCCAAGAAGA AGATCACCAG GTTGAAGAAG TCCGTACGTG TATATCGCAC TTCAAAGAAG 
0301 GCGGAGAGAG AAGTCGCAGA GAATGATCTT GAAGGAGTCC CAGTAGTTGG TCAAGATATC 
0361 AATCCGAATG CTATTCCGCT GGAGCAACAA GTTCAAAACC TATCACTTAG TAAAACCCCT 
0421 GCACCCAACA ACCCTTCACA AGCAAAAACA GTACATGCCA TTCACTCACG GCGATTTAGA 
0481 AGCTATTGTG ACAACTGCAC C

in silico PCR Fragment

0001 ATAAGGAGGA GCAGGAATCA GGATGTCCAG GATACAGCGA TCAAGATTAC TCCACGCAGC 
0061 TCCAAGTACA AAAACCAGCA CAGGAAACGG GAGCAGCAAA CCTCTCTTCT GGACTTTGTC 
0121 ATCAAGCCGA GACCAAAAAC ACAAAGGCAA ACAAAGGCAC ATAAACTTCA AAAGACACAT 
0181 TTAGCCATAA CAAGAGGTAG CTACATAGTT TACAAACCCA AGGGCAAAAC CCGCCTTGAT 
0241 CCCAAGAAGA AGATCACCAG GTTGAAGAAG TCCGTACGTG TATATCGCAC TTCAAAGAAG 
0301 GCGGAGAGAG AAGTCGCAGA GAATGATCTT GAAGGAGTCC CAGTAGTTGG TCAAGATATC 
0361 AATCCGAATG CTATTCCGCT GGAGCAACAA GTTCAAAACC TATCACTTAG TAAAACCCCT 
0421 GCACCCAACA ACCCTTCACA AGCAAAAACA GTACATGCCA TTCACTCACG GCGATTTAGA 
0481 AGCTATTGTG ACAACTGCAC C

Assemble Data


7066R-1.IR_full       1   ATAAGGAGGAGCAGGAATCAGGATGTCCAGGATACAGCGATCAAGATTACTCCACGCAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     1   ATAAGGAGGAGCAGGAATCAGGATGTCCAGGATACAGCGATCAAGATTACTCCACGCAGC 60


7066R-1.IR_full       61  TCCAAGTACAAAAACCAGCACAGGAAACGGGAGCAGCAAACCTCTCTTCTGGACTTTGTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     61  TCCAAGTACAAAAACCAGCACAGGAAACGGGAGCAGCAAACCTCTCTTCTGGACTTTGTC 120


7066R-1.IR_full       121 ATCAAGCCGAGACCAAAAACACAAAGGCAAACAAAGGCACATAAACTTCAAAAGACACAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     121 ATCAAGCCGAGACCAAAAACACAAAGGCAAACAAAGGCACATAAACTTCAAAAGACACAT 180


7066R-1.IR_full       181 TTAGCCATAACAAGAGGTAGCTACATAGTTTACAAACCCAAGGGCAAAACCCGCCTTGAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     181 TTAGCCATAACAAGAGGTAGCTACATAGTTTACAAACCCAAGGGCAAAACCCGCCTTGAT 240


7066R-1.IR_full       241 CCCAAGAAGAAGATCACCAGGTTGAAGAAGTCCGTACGTGTATATCGCACTTCAAAGAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     241 CCCAAGAAGAAGATCACCAGGTTGAAGAAGTCCGTACGTGTATATCGCACTTCAAAGAAG 300


7066R-1.IR_full       301 GCGGAGAGAGAAGTCGCAGAGAATGATCTTGAAGGAGTCCCAGTAGTTGGTCAAGATATC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     301 GCGGAGAGAGAAGTCGCAGAGAATGATCTTGAAGGAGTCCCAGTAGTTGGTCAAGATATC 360


7066R-1.IR_full       361 AATCCGAATGCTATTCCGCTGGAGCAACAAGTTCAAAACCTATCACTTAGTAAAACCCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     361 AATCCGAATGCTATTCCGCTGGAGCAACAAGTTCAAAACCTATCACTTAGTAAAACCCCT 420


7066R-1.IR_full       421 GCACCCAACAACCCTTCACAAGCAAAAACAGTACATGCCATTCACTCACGGCGATTTAGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7066R-1.in silico     421 GCACCCAACAACCCTTCACAAGCAAAAACAGTACATGCCATTCACTCACGGCGATTTAGA 480


7066R-1.IR_full       481 AGCTATTGTGACAACTGCACC 501
                          |||||||||||||||||||||
7066R-1.in silico     481 AGCTATTGTGACAACTGCACC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	483	0	0	0	NM_139947.2	CG7066-RA (CG7066), mRNA
0	0	4	3	2	NM_170256.1	CG8384-RC, transcript variant C (gro), mRNA
0	0	2	6	6	NM_165738.1	CG2269-RC, transcript variant C (CG2269), mRNA
0	0	2	6	6	NM_136709.2	CG2269-RA, transcript variant A (CG2269), mRNA
0	0	2	6	6	NM_165737.1	CG2269-RB, transcript variant B (CG2269), mRNA
0	0	1	5	3	NM_166516.1	CG11170-RB (CG11170), mRNA
0	0	1	4	4	NM_165879.1	CG8841-RB, transcript variant B (CG8841), mRNA
0	0	1	4	4	NM_136916.2	CG8841-RA, transcript variant A (CG8841), mRNA
0	0	1	4	4	NM_165880.1	CG8841-RC, transcript variant C (CG8841), mRNA
0	0	1	4	3	NM_142221.1	CG18516-RA (CG18516), mRNA
0	0	1	3	4	NM_001014613.2	CG17117-RE, transcript variant E (hth), mRNA
0	0	1	2	3	NM_136055.2	CG10376-RA (CG10376), mRNA
0	0	1	2	2	NM_167969.1	CG32488-RA (CG32488), mRNA
0	0	1	2	2	NM_143677.2	CG11360-RA (CG11360), mRNA
0	0	0	7	5	NM_140296.2	CG5642-RA (CG5642), mRNA
0	0	0	7	5	NM_136705.1	CG2292-RA (CG2292), mRNA
0	0	0	6	4	NM_140927.2	CG6981-RA, transcript variant A (CG6981), mRNA
0	0	0	6	4	NM_168831.1	CG6981-RB, transcript variant B (CG6981), mRNA
0	0	0	5	4	NM_143761.2	CG10241-RA (Cyp6a17), mRNA
0	0	0	5	2	NM_078747.2	CG2969-RA, transcript variant A (Atet), mRNA
0	0	0	5	2	NM_164580.1	CG2969-RB, transcript variant B (Atet), mRNA
0	0	0	4	9	NM_079460.2	CG18803-RA, transcript variant A (Psn), mRNA
0	0	0	4	9	NM_168856.1	CG18803-RB, transcript variant B (Psn), mRNA
0	0	0	4	8	NM_140436.1	CG8100-RA (CG8100), mRNA
0	0	0	4	5	NM_130594.1	CG14803-RA (CG14803), mRNA
0	0	0	4	5	NM_078604.3	CG32592-RA (hiw), mRNA
0	0	0	4	4	NM_166063.1	CG10145-RA (mspo), mRNA
0	0	0	4	3	NM_079408.4	CG5492-RB, transcript variant B (Tsp74F), mRNA
0	0	0	4	3	NM_168754.1	CG5492-RA, transcript variant A (Tsp74F), mRNA
0	0	0	4	3	NM_142894.1	CG10175-RC, transcript variant C (CG10175), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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