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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7031R-3 |
| Symbol |
CG7031 |
Full Name |
CG7031 |
| CG No |
CG7031 |
Old CG No |
CG7031 |
| Synonyms |
CG7031 |
| Accession No (Link to NCBI) |
NM_142844.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCCTCGACAGAGACGGCCAA |
| Primer Seq. 3' |
aatctagaggtaccCGTAGGCTGGCAAAAATCCAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATCCTCGACA GNGACGGCCA AGAACCAGCC ATTGTATCAC AATATGGGGT CTTTGTTCGA
0061 GAATCGATGG CTGCCAATGG ATCAGCCGAT GCAGCATCAT CCCAATCATC GGTATGGCTC
0121 GTCCAAAATT CCACGGGCAA ATGCGGGTGA AAGGAGGGCC TTTAGCAGCG GCCATTCCTC
0181 AGGCTCCCNG CAAGGATTGG CCCATATGCT CCAACGGACT AACAAATCTC TCGGGCCTGG
0241 GACACATTTC CAATGGGTAT GGCTCGGTGG CGCCCACCCA TACGGAGGCA CTGGCCCTGG
0301 GCTCCACCAA ACAGGAGATA CCGATTTACG AGGACCACAA TGAAGATGCC GCCGCGGTGG
0361 CTGCCGTTGC AGCAGCTTGG AGCCCATGAT TTTGGCTCGG GAAATGAACA ATCTGACGGT
0421 GGATTGGAGA ACAATCAAGA ACATTCGCCG ATTTACAATT ATCCCGGTGC CGCACATAAT
0481 GGTGGATTTT TGCCAGCCTA CG |
| in silico PCR Fragment |
0001 ATCCTCGACA GAGACGGCCA AGAACCAGCC ATTGTATCAC AATATGGGGT CTTTGTTCGA
0061 GAATCGATGG CTGCCAATGG ATCAGCCGAT GCAGCATCAT CCCAATCATC GGTATGGCTC
0121 GTCCAAAATT CCACGGGCAA ATGCGGGTGA AAGGAGGGCC TTTAGCAGCG GCCATTCCTC
0181 AGGCTCCCGG CAAGGATTGG CCCATATGCT -CAACGGACT AACAAATCTC TCGGGCCTGG
0241 GACACATTTC CAATGGGTAT GGCTCGGTGG CGCCCACCCA TACGGAGGCA CTGGCCCTGG
0301 GCTCCACCAA ACAGGAGATA CCGATTTACG AGGACCACAA TGAAGATGCC ACCGCGGTGG
0361 CTGCCGTTGC AGCAGC-TGG AGCCCATGAT TTTGGCTCGG GAAATGAACA ATCTGGCGGT
0421 GGATTGGAGA ACAATCAAGA ACATTCGCCG ATTTACAATT ATCCCGGTGC CGCACATAAT
0481 GGTGGATTTT TGCCAGCCTA CG |
| Assemble Data |
7031R-3.IR_full 1 ATCCTCGACAGNGACGGCCAAGAACCAGCCATTGTATCACAATATGGGGTCTTTGTTCGA 60
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
7031R-3.in silico 1 ATCCTCGACAGAGACGGCCAAGAACCAGCCATTGTATCACAATATGGGGTCTTTGTTCGA 60
7031R-3.IR_full 61 GAATCGATGGCTGCCAATGGATCAGCCGATGCAGCATCATCCCAATCATCGGTATGGCTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7031R-3.in silico 61 GAATCGATGGCTGCCAATGGATCAGCCGATGCAGCATCATCCCAATCATCGGTATGGCTC 120
7031R-3.IR_full 121 GTCCAAAATTCCACGGGCAAATGCGGGTGAAAGGAGGGCCTTTAGCAGCGGCCATTCCTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7031R-3.in silico 121 GTCCAAAATTCCACGGGCAAATGCGGGTGAAAGGAGGGCCTTTAGCAGCGGCCATTCCTC 180
7031R-3.IR_full 181 AGGCTCCCNGCAAGGATTGGCCCATATGCTCCAACGGACTAACAAATCTCTCGGGCCTGG 240
|||||||| ||||||||||||||||||||| |||||||||||||||||||||||||||||
7031R-3.in silico 181 AGGCTCCCGGCAAGGATTGGCCCATATGCT-CAACGGACTAACAAATCTCTCGGGCCTGG 240
7031R-3.IR_full 241 GACACATTTCCAATGGGTATGGCTCGGTGGCGCCCACCCATACGGAGGCACTGGCCCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7031R-3.in silico 241 GACACATTTCCAATGGGTATGGCTCGGTGGCGCCCACCCATACGGAGGCACTGGCCCTGG 300
7031R-3.IR_full 301 GCTCCACCAAACAGGAGATACCGATTTACGAGGACCACAATGAAGATGCCGCCGCGGTGG 360
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
7031R-3.in silico 301 GCTCCACCAAACAGGAGATACCGATTTACGAGGACCACAATGAAGATGCCACCGCGGTGG 360
7031R-3.IR_full 361 CTGCCGTTGCAGCAGCTTGGAGCCCATGATTTTGGCTCGGGAAATGAACAATCTGACGGT 420
|||||||||||||||| |||||||||||||||||||||||||||||||||||||| ||||
7031R-3.in silico 361 CTGCCGTTGCAGCAGC-TGGAGCCCATGATTTTGGCTCGGGAAATGAACAATCTGGCGGT 420
7031R-3.IR_full 421 GGATTGGAGAACAATCAAGAACATTCGCCGATTTACAATTATCCCGGTGCCGCACATAAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7031R-3.in silico 421 GGATTGGAGAACAATCAAGAACATTCGCCGATTTACAATTATCCCGGTGCCGCACATAAT 480
7031R-3.IR_full 481 GGTGGATTTTTGCCAGCCTACG 502
||||||||||||||||||||||
7031R-3.in silico 481 GGTGGATTTTTGCCAGCCTACG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_142844.1 |
CG7031-RA (CG7031), mRNA |
| 0.2 |
1 |
2 |
6 |
9 |
NM_001043153.1 |
CG32434-RC, transcript variant C (siz), mRNA |
| 0.2 |
1 |
2 |
6 |
9 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0.2 |
1 |
2 |
6 |
9 |
NM_168881.1 |
CG32434-RB, transcript variant B (siz), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_166402.1 |
CG30147-RB, transcript variant B (Hil), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_137649.2 |
CG30147-RA, transcript variant A (Hil), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165353.2 |
CG9266-RB (CG9266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164733.1 |
CG11266-RD, transcript variant D (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164735.1 |
CG11266-RG, transcript variant G (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164730.1 |
CG11266-RA, transcript variant A (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164732.1 |
CG11266-RC, transcript variant C (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164734.1 |
CG11266-RF, transcript variant F (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_135251.2 |
CG11266-RB, transcript variant B (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_164731.1 |
CG11266-RE, transcript variant E (CG11266), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_057358.3 |
CG3180-RA (RpII140), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_132076.5 |
CG3566-RB, transcript variant B (CG3566), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_206632.1 |
CG3566-RC, transcript variant C (CG3566), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169191.2 |
CG31146-RD (CG31146), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140330.1 |
CG14121-RA (CG14121), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_133155.2 |
CG12200-RA (CG12200), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139742.1 |
CG13288-RA (CG13288), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_166137.1 |
CG8405-RA (CG8405), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164646.1 |
CG7269-RA, transcript variant A (Hel25E), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164648.1 |
CG7269-RC, transcript variant C (Hel25E), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164647.1 |
CG7269-RB, transcript variant B (Hel25E), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206391.1 |
CG33256-RA (CG33256), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_130550.2 |
CG11417-RA (CG11417), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169453.1 |
CG10095-RA (dpr15), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143524.2 |
CG9743-RA (CG9743), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136117.2 |
CG17544-RA, transcript variant A (CG17544), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
 |
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JNK signal
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Caspase-3 signal
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