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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7009R-2 
 Symbol CG7009  Full Name CG7009 
 CG No CG7009  Old CG No CG7009 
 Synonyms CG7009 
 Accession No (Link to NCBI) NM_142690.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Jezowska B, Fernández BG, Amândio AR, Duarte P, Mendes C, Brás-Pereira C, Janody F.
A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis.
Dev Biol (2011) 360(1) 143-59 [ PubMed ID = 21963538 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGGGCAGGACTTCGAAGGATA 
 Primer Seq. 3' 
aatctagaggtaccGTCTGCCCGGTAGATCTTGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGGGCAGGAC TTCGAAGGAT AAACGAGACA TATTCTACCG CCTGGCCAAG GAGCAGGGAT 
0061 GGCGTGCACG AAGTGCTTTC AAACTGCTCC AGGCAGATGA GACCTTTCAG TTGCTAGAAG 
0121 GTCTCACCCG AGCCGTGGAC CTTTGCGCCG CTCCTGGCAG CTGGTCACAG GTGCTGGCCA 
0181 AACGCTTGTA CGAGCCTTTG CCGCCGGAGG AACGGGAAAA GGTAAAGATC ATCGCCGTAG 
0241 ACCTGCAGGG AATGGCTCCG ATCGAAGGAG TCAAACAACT GCGGGCGGAC ATCAGCAAGG 
0301 AGTCGACAGC CGAGGCCATA ATTGAGTTCT TTGGCGGCGA GAAGGCACAG ATTGTTGTTA 
0361 GCGATGGCGC TCCCGATTCT ACTGGAATGC ACGACTTTGA CTCGTACGTT CAGGGCGAAC 
0421 TACTTCTTTC CGCCCTCAGC ATATCCACCT TCATACTGGA AGAGGGAGGC TCCTTCGTGT 
0481 CCAAGATCTA CCGGGCATGA C 
 in silico PCR Fragment 
0001 TGGGCAGGAC TTCGAAGGAT AAACGAGACA TATTCTACCG CCTGGCCAAG GAGCAGGGAT 
0061 GGCGTGCACG AAGTGCTTTC AAACTGCTCC AGGCGGATGA GACCTTTCAG TTGCTAGAAG 
0121 GTCTCACCCG AGCCGTGGAC CTTTGCGCCG CTCCTGGCAG CTGGTCACAG GTGCTGGCCA 
0181 AACGCTTGTA CGAGCCTCTG CCGCCAGAGG AACGGGAAAA GGTAAAGATC ATCGCCGTAG 
0241 ACCTGCAGGG AATGGCTCCG ATCGAAGGAG TCAAACAACT GCGGGCGGAC ATCAGCAAGG 
0301 AGTCGACAGC CGAGGCCATA ATTGAGTTCT TTGGCGGCGA GAAGGCACAG ATTGTTGTTA 
0361 GCGATGGCGC TCCCGATTCT ACTGGAATGC ACGACTTTGA CTCGTACGTT CAGGGCGAAC 
0421 TACTTCTCTC CGCCCTCAGC ATATCCACCT TCATACTGGA AGAGGGAGGC TCCTTCGTGT 
0481 CCAAGATCTA CCGGGCA-GA C 
 Assemble Data 

7009R-2.IR_full       1   TGGGCAGGACTTCGAAGGATAAACGAGACATATTCTACCGCCTGGCCAAGGAGCAGGGAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     1   TGGGCAGGACTTCGAAGGATAAACGAGACATATTCTACCGCCTGGCCAAGGAGCAGGGAT 60


7009R-2.IR_full       61  GGCGTGCACGAAGTGCTTTCAAACTGCTCCAGGCAGATGAGACCTTTCAGTTGCTAGAAG 120
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
7009R-2.in silico     61  GGCGTGCACGAAGTGCTTTCAAACTGCTCCAGGCGGATGAGACCTTTCAGTTGCTAGAAG 120


7009R-2.IR_full       121 GTCTCACCCGAGCCGTGGACCTTTGCGCCGCTCCTGGCAGCTGGTCACAGGTGCTGGCCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     121 GTCTCACCCGAGCCGTGGACCTTTGCGCCGCTCCTGGCAGCTGGTCACAGGTGCTGGCCA 180


7009R-2.IR_full       181 AACGCTTGTACGAGCCTTTGCCGCCGGAGGAACGGGAAAAGGTAAAGATCATCGCCGTAG 240
                          ||||||||||||||||| ||||||| ||||||||||||||||||||||||||||||||||
7009R-2.in silico     181 AACGCTTGTACGAGCCTCTGCCGCCAGAGGAACGGGAAAAGGTAAAGATCATCGCCGTAG 240


7009R-2.IR_full       241 ACCTGCAGGGAATGGCTCCGATCGAAGGAGTCAAACAACTGCGGGCGGACATCAGCAAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     241 ACCTGCAGGGAATGGCTCCGATCGAAGGAGTCAAACAACTGCGGGCGGACATCAGCAAGG 300


7009R-2.IR_full       301 AGTCGACAGCCGAGGCCATAATTGAGTTCTTTGGCGGCGAGAAGGCACAGATTGTTGTTA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     301 AGTCGACAGCCGAGGCCATAATTGAGTTCTTTGGCGGCGAGAAGGCACAGATTGTTGTTA 360


7009R-2.IR_full       361 GCGATGGCGCTCCCGATTCTACTGGAATGCACGACTTTGACTCGTACGTTCAGGGCGAAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     361 GCGATGGCGCTCCCGATTCTACTGGAATGCACGACTTTGACTCGTACGTTCAGGGCGAAC 420


7009R-2.IR_full       421 TACTTCTTTCCGCCCTCAGCATATCCACCTTCATACTGGAAGAGGGAGGCTCCTTCGTGT 480
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
7009R-2.in silico     421 TACTTCTCTCCGCCCTCAGCATATCCACCTTCATACTGGAAGAGGGAGGCTCCTTCGTGT 480


7009R-2.IR_full       481 CCAAGATCTACCGGGCATGAC 501
                          ||||||||||||||||| |||
7009R-2.in silico     481 CCAAGATCTACCGGGCA-GAC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_142690.1  CG7009-RA (CG7009), mRNA 
0   0  10  5  28  NM_142333.1  CG5220-RA (CG5220), mRNA 
0   0  3  2  2  NM_001015156.1  CG17420-PB.3 (CG17420), mRNA 
0   0  3  2  2  NM_001015155.1  CG17420-PA.3 (CG17420), mRNA 
0   0  2  3  4  NM_140430.2  CG6603-RA, transcript variant A (Hsc70Cb), mRNA 
0   0  2  3  4  NM_168573.1  CG6603-RB, transcript variant B (Hsc70Cb), mRNA 
0   0  2  3  4  NM_168574.1  CG6603-RC, transcript variant C (Hsc70Cb), mRNA 
0   0  2  2  4  NM_143175.1  CG17770-RA (CG17770), mRNA 
0   0  1  8  2  NM_166083.1  CG8095-RA, transcript variant A (scb), mRNA 
0   0  1  8  2  NM_079026.2  CG8095-RB, transcript variant B (scb), mRNA 
0   0  1  4  4  NM_206091.1  CG7734-RD, transcript variant D (shn), mRNA 
0   0  1  4  4  NM_057375.3  CG7734-RA, transcript variant A (shn), mRNA 
0   0  1  4  4  NM_134279.2  CG7734-RB, transcript variant B (shn), mRNA 
0   0  1  4  4  NM_057376.2  CG7734-RC, transcript variant C (shn), mRNA 
0   0  1  4  3  NM_138102.1  CG13588-RA (CG13588), mRNA 
0   0  1  3  4  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  1  3  4  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  1  3  4  NM_206564.1  CG5794-RE, transcript variant E (CG5794), mRNA 
0   0  1  3  3  NM_079538.2  CG1108-RA (alpha-Est6), mRNA 
0   0  1  2  5  NM_137055.2  CG13338-RA (CG13338), mRNA 
0   0  1  2  4  NM_137564.2  CG7417-RA (Tab2), mRNA 
0   0  1  2  3  NM_136537.1  CG8693-RA (CG8693), mRNA 
0   0  1  2  3  NM_132192.1  CG1444-RA (CG1444), mRNA 
0   0  1  2  2  NM_079543.2  CG1257-RA (alpha-Est3), mRNA 
0   0  0  10  3  NM_142578.2  CG11447-RA (CG11447), mRNA 
0   0  0  5  2  NM_142237.2  CG4606-RA (alpha-Man-IIb), mRNA 
0   0  0  4  8  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  0  4  6  NM_166017.1  CG18076-RE, transcript variant E (shot), mRNA 
0   0  0  4  6  NM_166016.1  CG18076-RB, transcript variant B (shot), mRNA 
0   0  0  4  6  NM_166015.1  CG18076-RG, transcript variant G (shot), mRNA 

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