NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6962R-3

Symbol CG6962 Full Name CG6962

CG No CG6962 Old CG No CG6962

Synonyms CG6962

Accession No (Link to NCBI) NM_141867.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCATGCCGCCAGAAAACCTAA

Primer Seq. 3'

aatctagaggtaccGGCTATGGGATTGATAGAGTG

Predicted Fragment Size

IR fragment full Seq

0001 CCATGCCGCC AGAAAACCTA AGCAGCCGCC TGTTGACGGT GCTCAGTTTG CCTCTATTCG 
0061 AGCGCGTGCA CGAGCTGAGC ATCCTGTTCG ATCGGTGCTC CTTGCGCCAA GTGCAGGAGA 
0121 TCTTCCCCCA CGTTGTGCAC TCCATCTTCG GGATAGATGG CAATCCGTTG GGCTGGGGCC 
0181 TGCGGACCAC GACGCTTGAA AACAATCCGG TGCAATTCCA AACGCTGCAC CAGTTCTTCG 
0241 GCGTCTGTGG TCCCCCTGAT GCATGTTTGC CACCGTCTGC TCGTCGACCA GTACAAATTC 
0301 GAACTAGACA TCAATCTGCT GCCGGCGAAA TTCGTTAGCC TGCTGCAGAA TGGTCAGAAT 
0361 CCTAGTTTCT ATGCAGAACT CATCAATGTG GAGACGATGA TGCACCAGGT GTCNCNCGCT 
0421 CtcgNTCAAC GCCTTCGACT TCTATGTGAT TCACTTTGTG CTATATGcCC TGCAGCCACT 
0481 ACACTCTATC AATCCCATAN Cc

in silico PCR Fragment

0001 CCATGCCGCC AGAAAACCTA AGCAGCCGCC TGTTGACGGT GCTCAGTTTG CCTCTATTCG 
0061 AGCGCGTGCA CGAGCTGAGC ATCCTGTTCG ATCGGTGCTC CTTGCGCCAA GTGCAGGAGA 
0121 TCTTCCCCCA CGTTGTGCAC TCCATCTTCG GGATAGATGG CAATCCGTTG GGCTGGGGCC 
0181 TGCGGACCAC GACGCTTGAA AACAATCCGG TGCAATTCCA AACGCTGCAC CAGTTCTTCG 
0241 GCGTCTGTGG T-CCCCTGAT GCATGTTTGC CACCGTCTGC TCGTCGACCA GTACAAATTC 
0301 GAACTAGACA TCAATCTGCT GCCGGCGAAA TTCGTTAGCC TGCTGCAGAA TGGTCAGAAT 
0361 CCTAGTTTCT ATGCAGAACT CATCAATGTG GAGACGATGA TGCACCAGGT GTC-CACGCT 
0421 CTCGCTCAAC GCCTTCGACT TCTATGTGAT TCACTTTGTG CTATATGCCC TGCAGCCACT 
0481 ACACTCTATC AATCCCATAG CC

Assemble Data


6962R-3.IR_full       1   CCATGCCGCCAGAAAACCTAAGCAGCCGCCTGTTGACGGTGCTCAGTTTGCCTCTATTCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     1   CCATGCCGCCAGAAAACCTAAGCAGCCGCCTGTTGACGGTGCTCAGTTTGCCTCTATTCG 60


6962R-3.IR_full       61  AGCGCGTGCACGAGCTGAGCATCCTGTTCGATCGGTGCTCCTTGCGCCAAGTGCAGGAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     61  AGCGCGTGCACGAGCTGAGCATCCTGTTCGATCGGTGCTCCTTGCGCCAAGTGCAGGAGA 120


6962R-3.IR_full       121 TCTTCCCCCACGTTGTGCACTCCATCTTCGGGATAGATGGCAATCCGTTGGGCTGGGGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     121 TCTTCCCCCACGTTGTGCACTCCATCTTCGGGATAGATGGCAATCCGTTGGGCTGGGGCC 180


6962R-3.IR_full       181 TGCGGACCACGACGCTTGAAAACAATCCGGTGCAATTCCAAACGCTGCACCAGTTCTTCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     181 TGCGGACCACGACGCTTGAAAACAATCCGGTGCAATTCCAAACGCTGCACCAGTTCTTCG 240


6962R-3.IR_full       241 GCGTCTGTGGTCCCCCTGATGCATGTTTGCCACCGTCTGCTCGTCGACCAGTACAAATTC 300
                          ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     241 GCGTCTGTGGT-CCCCTGATGCATGTTTGCCACCGTCTGCTCGTCGACCAGTACAAATTC 300


6962R-3.IR_full       301 GAACTAGACATCAATCTGCTGCCGGCGAAATTCGTTAGCCTGCTGCAGAATGGTCAGAAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     301 GAACTAGACATCAATCTGCTGCCGGCGAAATTCGTTAGCCTGCTGCAGAATGGTCAGAAT 360


6962R-3.IR_full       361 CCTAGTTTCTATGCAGAACTCATCAATGTGGAGACGATGATGCACCAGGTGTCNCNCGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| | ||||
6962R-3.in silico     361 CCTAGTTTCTATGCAGAACTCATCAATGTGGAGACGATGATGCACCAGGTGTC-CACGCT 420


6962R-3.IR_full       421 CTCGNTCAACGCCTTCGACTTCTATGTGATTCACTTTGTGCTATATGCCCTGCAGCCACT 480
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
6962R-3.in silico     421 CTCGCTCAACGCCTTCGACTTCTATGTGATTCACTTTGTGCTATATGCCCTGCAGCCACT 480


6962R-3.IR_full       481 ACACTCTATCAATCCCATANCC 502
                          ||||||||||||||||||| ||
6962R-3.in silico     481 ACACTCTATCAATCCCATAGCC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141867.2	CG6962-RA (CG6962), mRNA
0	0	3	4	4	NM_141318.2	CG2104-RA (CG2104), mRNA
0	0	3	2	3	NM_168429.2	CG32079-RA (CG32079), mRNA
0	0	2	4	4	NM_206678.1	CG33175-RA, transcript variant A (spri), mRNA
0	0	2	4	4	NM_176723.2	CG33175-RG, transcript variant G (spri), mRNA
0	0	2	3	3	NM_136131.2	CG10189-RA (CG10189), mRNA
0	0	1	7	9	NM_079572.2	CG9484-RA (hyd), mRNA
0	0	1	3	5	NM_078845.3	CG15274-RA (GABA-B-R1), mRNA
0	0	1	3	3	NM_132606.1	CG3754-RA (CG3754), mRNA
0	0	1	3	2	NM_140232.2	CG11660-RA, transcript variant A (CG11660), mRNA
0	0	1	3	2	NM_168469.1	CG11660-RB, transcript variant B (CG11660), mRNA
0	0	1	3	2	NM_141738.3	CG6303-RA (Bruce), mRNA
0	0	1	2	5	NM_139839.3	CG32381-RA (unc-13-4A), mRNA
0	0	1	2	3	NM_139604.2	CG14998-RC, transcript variant C (CG14998), mRNA
0	0	1	2	3	NM_168064.2	CG14998-RA, transcript variant A (CG14998), mRNA
0	0	1	2	3	NM_168063.2	CG14998-RD, transcript variant D (CG14998), mRNA
0	0	1	2	3	NM_168062.2	CG14998-RE, transcript variant E (CG14998), mRNA
0	0	1	2	3	NM_168061.2	CG14998-RB, transcript variant B (CG14998), mRNA
0	0	1	2	2	NM_079259.2	CG4974-RA (dally), mRNA
0	0	1	2	2	NM_170519.1	CG31010-RA (CG31010), mRNA
0	0	0	5	6	NM_169882.1	CG4836-RB, transcript variant B (CG4836), mRNA
0	0	0	5	6	NM_169883.1	CG4836-RA, transcript variant A (CG4836), mRNA
0	0	0	5	6	NM_142599.1	CG4836-RC, transcript variant C (CG4836), mRNA
0	0	0	5	5	NM_168863.1	CG32425-RC, transcript variant C (CG32425), mRNA
0	0	0	5	5	NM_168861.1	CG32425-RE, transcript variant E (CG32425), mRNA
0	0	0	5	5	NM_168860.1	CG32425-RA, transcript variant A (CG32425), mRNA
0	0	0	5	3	NM_167346.1	CG4353-RA, transcript variant A (hep), mRNA
0	0	0	5	3	NM_078587.2	CG4353-RC, transcript variant C (hep), mRNA
0	0	0	4	5	NM_170524.1	CG12072-RA (wts), mRNA
0	0	0	4	5	NM_001043290.1	CG34100-RB, transcript variant B (mld), mRNA

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