NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6952R-3

Symbol CG32771 Full Name CG32771

CG No CG32771 Old CG No CG6952

Synonyms CG6952, CG32771

Accession No (Link to NCBI) NM_167022.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCAGTGGCGAGTTTGTTTACG

Primer Seq. 3'

aatctagaggtaccGGTCAAAACGGTCAGCGAATA

Predicted Fragment Size

IR fragment full Seq

0001 CCAGNTGGCG AGTTTGTTTA CGGCCTGGAT GGCATGGATG CCACCGATCT GGAGGCGGGC 
0061 GGAATGCCCC AGTTTGCCCT CTCGCCGGAC ACATACGATG TGCGACAGCG GACCATTGAG 
0121 AATATATGGG ACATCACCGT CTCGCTCAAC ATTCTGTACA AGGAGAACTG GACAAAATTA 
0181 GCCGCACTTG AAATAGCCAA GTTTCAAGAC CAATTAATCA AGAGACTGAA CGAGGATGTT 
0241 ATGCTGCAAT TATCGCACGA CGATGTGGCC AACGCCCCCT CCTCCTCCTC CTCCTCCTCA 
0301 TCCTCGTCGT CATCGTCGTC GTNCCTCGAC GGGCGCCTCT GGTGTTCCGG GTAGCAACAA 
0361 TCCGGCCACG GAAGCCGTGC TCCTTCACAC ACACTATCAC CATCATCGTG CCGGAGGCGT 
0421 TGGGGGCGTG GCAGTGGGCG GTGGACCGCC GCATGAGTGG AATTTTGCCA AAGCGTTTTT 
0481 GTATTCGCTG ACCGTTTTGA NC

in silico PCR Fragment

0001 CCAG-TGGCG AGTTTGTTTA CGGCCTGGAT GGCATGGATG CCACCGATTT GGAGGCGGGC 
0061 GGAATGCCCC AGTTTGCCCT CTCGCCGGAC ACATACGATG TGCGACAGCG GACCATTGAG 
0121 AATATATGGG ACATTACCGT CTCGCTCAAC ATTCTGTACA AGGAGAACTG GACAAAATTA 
0181 GCCGCACTTG AAATAGCCAA GTTTCAAGAC CAATTAATCA AGAGACTGAA CGAGGATGTT 
0241 ATGCTGCAAT TATCGCACGA CGATGTGGCC AACGCCCCCT CCTCCTCCTC CTCCTCCTCA 
0301 TCCTCGTCGT CATCGTCGTC GT-CCTCGAC GGGCGCCTCT GGTGTTCCGG GTAGCAACAA 
0361 TCCGGCCACG GAAGCCGTGC TCCTTCACAC ACACTATCAC CATCATCGTG CCGGAGGCGT 
0421 TGGGGGCGTG GCAGTGGGCG GTGGACCGCC GCATGAGTGG AATTTTGCCA AAGCGTTTTT 
0481 GTATTCGCTG ACCGTTTTGA CC

Assemble Data


6952R-3.IR_full       1   CCAGNTGGCGAGTTTGTTTACGGCCTGGATGGCATGGATGCCACCGATCTGGAGGCGGGC 60
                          |||| ||||||||||||||||||||||||||||||||||||||||||| |||||||||||
6952R-3.in silico     1   CCAG-TGGCGAGTTTGTTTACGGCCTGGATGGCATGGATGCCACCGATTTGGAGGCGGGC 60


6952R-3.IR_full       61  GGAATGCCCCAGTTTGCCCTCTCGCCGGACACATACGATGTGCGACAGCGGACCATTGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     61  GGAATGCCCCAGTTTGCCCTCTCGCCGGACACATACGATGTGCGACAGCGGACCATTGAG 120


6952R-3.IR_full       121 AATATATGGGACATCACCGTCTCGCTCAACATTCTGTACAAGGAGAACTGGACAAAATTA 180
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     121 AATATATGGGACATTACCGTCTCGCTCAACATTCTGTACAAGGAGAACTGGACAAAATTA 180


6952R-3.IR_full       181 GCCGCACTTGAAATAGCCAAGTTTCAAGACCAATTAATCAAGAGACTGAACGAGGATGTT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     181 GCCGCACTTGAAATAGCCAAGTTTCAAGACCAATTAATCAAGAGACTGAACGAGGATGTT 240


6952R-3.IR_full       241 ATGCTGCAATTATCGCACGACGATGTGGCCAACGCCCCCTCCTCCTCCTCCTCCTCCTCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     241 ATGCTGCAATTATCGCACGACGATGTGGCCAACGCCCCCTCCTCCTCCTCCTCCTCCTCA 300


6952R-3.IR_full       301 TCCTCGTCGTCATCGTCGTCGTNCCTCGACGGGCGCCTCTGGTGTTCCGGGTAGCAACAA 360
                          |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
6952R-3.in silico     301 TCCTCGTCGTCATCGTCGTCGT-CCTCGACGGGCGCCTCTGGTGTTCCGGGTAGCAACAA 360


6952R-3.IR_full       361 TCCGGCCACGGAAGCCGTGCTCCTTCACACACACTATCACCATCATCGTGCCGGAGGCGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     361 TCCGGCCACGGAAGCCGTGCTCCTTCACACACACTATCACCATCATCGTGCCGGAGGCGT 420


6952R-3.IR_full       421 TGGGGGCGTGGCAGTGGGCGGTGGACCGCCGCATGAGTGGAATTTTGCCAAAGCGTTTTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6952R-3.in silico     421 TGGGGGCGTGGCAGTGGGCGGTGGACCGCCGCATGAGTGGAATTTTGCCAAAGCGTTTTT 480


6952R-3.IR_full       481 GTATTCGCTGACCGTTTTGANC 502
                          |||||||||||||||||||| |
6952R-3.in silico     481 GTATTCGCTGACCGTTTTGACC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100.41	484	24	40	92	NM_167022.1	CG32771-RA (CG32771), mRNA
6.22	30	84	76	61	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
6.22	30	84	76	61	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
3.52	17	43	42	58	NM_135975.2	CG31781-RB (CG31781), mRNA
3.11	15	53	58	62	NM_170053.2	CG17894-RC, transcript variant C (cnc), mRNA
2.9	14	41	67	117	NM_133114.2	CG32541-RA (CG32541), mRNA
2.69	13	35	34	44	NM_176374.1	CG4761-RA (knrl), mRNA
2.48	12	42	65	97	NM_167309.1	CG32663-RA (CG32663), mRNA
2.48	12	41	69	78	NM_078516.2	CG12690-RA (CHES-1-like), mRNA
2.28	11	38	48	55	NM_140288.2	CG6801-RA (l(3)j2D3), mRNA
2.28	11	36	65	97	NM_133104.2	CG7282-RA (CG7282), mRNA
2.28	11	31	33	49	NM_057368.3	CG2621-RD, transcript variant D (sgg), mRNA
2.28	11	31	31	29	NM_138057.1	CG13576-RA (CG13576), mRNA
2.28	11	30	34	43	NM_143222.1	CG14237-RA (CG14237), mRNA
2.28	11	4	20	99	NM_167387.1	CG32611-RB (CG32611), mRNA
2.07	10	35	55	75	NM_057406.3	CG6222-RA (su(s)), mRNA
2.07	10	34	41	43	NM_135079.4	CG14021-RB, transcript variant B (CG14021), mRNA
2.07	10	34	41	43	NM_164640.1	CG14021-RA, transcript variant A (CG14021), mRNA
2.07	10	33	38	37	NM_135495.2	CG4778-RA (CG4778), mRNA
1.86	9	52	151	267	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
1.86	9	52	151	267	NM_001014739.1	CG7107-RE, transcript variant E (up), mRNA
1.86	9	52	151	267	NM_001014738.1	CG7107-RF, transcript variant F (up), mRNA
1.86	9	52	151	267	NM_167375.1	CG7107-RB, transcript variant B (up), mRNA
1.86	9	52	151	267	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
1.86	9	52	151	267	NM_001014737.1	CG7107-RG, transcript variant G (up), mRNA
1.86	9	41	85	129	NM_167340.1	CG32648-RA (Pde9), mRNA
1.86	9	32	53	60	NM_141658.3	CG8301-RA (CG8301), mRNA
1.86	9	26	28	29	NM_134510.1	CG12703-RA (CG12703), mRNA
1.65	8	43	72	85	NM_167620.2	CG32547-RA (CG32547), mRNA
1.65	8	28	34	54	NM_206283.1	CG8398-RD, transcript variant D (CG8398), mRNA

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