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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6831R-2 |
| Symbol |
rhea |
Full Name |
rhea |
| CG No |
CG6831 |
Old CG No |
CG6831 |
| Synonyms |
rhea, talin, Talin, CG6831, CT21159, Tln |
| Accession No (Link to NCBI) |
NM_139981.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Perkins AD, Ellis SJ, Asghari P, Shamsian A, Moore ED, Tanentzapf G.
Integrin-mediated adhesion maintains sarcomeric integrity.
Dev Biol (2010) 338(1) 15-27
[
PubMed ID = 19879257
]
[
RRC reference
]
Kókai E, Páldy FS, Somogyi K, Chougule A, Pál M, Kerekes É, Deák P, Friedrich P, Dombrádi V, Ádám G.
CalpB modulates border cell migration in Drosophila egg chambers.
BMC Dev Biol (2012) 12 20
[
PubMed ID = 22827336
]
[
RRC reference
]
Xie X, Auld VJ.
Integrins are necessary for the development and maintenance of the glial layers in the Drosophila peripheral nerve.
Development (2011) 138(17) 3813-22
[
PubMed ID = 21828098
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCATACAGTTCCAGCCCAACA |
| Primer Seq. 3' |
aatctagaggtaccCACATCCACCCAGTTCATTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCATACAGTT CCAGCCCAAC ACCACAGTCT TCGANTGCCT NGCAAGGTCC ATTCGCGATA
0061 AGTTTCCGCC GAGGCCAGTG CAAGGACNAA CCCAGCGAAT ATGGNACTGT TTATCTCTGA
0121 NTGAGCCAGA ACCAGCAGGG GGTTTGGTTG NAACCCGGGA CGCACCCTGG GGCTACTACA
0181 TACTGCACAA CCCAGGATAC GCTGGAGTAT CGCCGCAAAG ACCACNNAAA CCCTACGTGT
0241 TCNCATGTTG GATGGCGCTG TAAAGACCAT TCTGGTGGAT GACTNCCCAG CCGGTGTCGC
0301 AGCTTATGGT GGTCATCTGC ACAAAGATCG GCATCACCAA TCATGAGGAA TATGGATTGG
0361 TGCGCGAGGA CAATGAGGCG CAGAATGAGA ATCTGCCGGA CAACAAGTTT GGAACGCTTA
0421 CCCTTAAGCG TAAGATTATG GAGAAGGATC gCGATGCCAA AATGGAGAGT TTGCGCAAGA
0481 AACTAAAGAC GGACGATGAA ATGAACTGGG TGGATGTG |
| in silico PCR Fragment |
0001 CCATACAGTT CCAGCCCAAC ACCACAGTCT TCGA-TGCCT -GCAAGGT-C ATTCGCGATA
0061 AGTT--CGCC GAGG-CAGTG CAAGGAC-AA CCCAGCGAAT ATGG-ACTGT TTATCTCTGA
0121 -TGAG-CAGA ACCAGCAGGG GGTTTGGTTG GAA-CCGGGA CGCACCCT-G GGCTACTACA
0181 TACTGCACAA -CCAGGATAC GCTGGAGTAT CGCCGC-AAG A-CAC--GAA CCCTACGTGT
0241 TCGTATGTTG GATGGCGCTG TAAAGACCAT TCTGGTGGAT GACT-CCCAG CCGGTGTCGC
0301 AGCTTATGGT GGTCATCTGC ACAAAGATCG GCATCACCAA TCATGAGGAA TATGGATTGG
0361 TGCGCGAGGA CAATGAGGCG CAGAATGAGA ATCTGCCGGA CAACAAGTTT GGAACGCTTA
0421 CCCTTAAGCG TAAGATTATG GAGAAGGATC GCGATGCCAA AATGGAGAGT TTGCGCAAGA
0481 AACTAAAGAC GGACGATGAA ATGAACTGGG TGGATGTG |
| Assemble Data |
6831R-2.IR_full 1 CCATACAGTTCCAGCCCAACACCACAGTCTTCGANTGCCTNGCAAGGTCCATTCGCGATA 60
|||||||||||||||||||||||||||||||||| ||||| ||||||| |||||||||||
6831R-2.in silico 1 CCATACAGTTCCAGCCCAACACCACAGTCTTCGA-TGCCT-GCAAGGT-CATTCGCGATA 60
6831R-2.IR_full 61 AGTTTCCGCCGAGGCCAGTGCAAGGACNAACCCAGCGAATATGGNACTGTTTATCTCTGA 120
|||| |||||||| |||||||||||| |||||||||||||||| |||||||||||||||
6831R-2.in silico 61 AGTT--CGCCGAGG-CAGTGCAAGGAC-AACCCAGCGAATATGG-ACTGTTTATCTCTGA 120
6831R-2.IR_full 121 TGAGCCAGAACCAGCAGGGGGTTTGGTTGNAACCCGGGACGCACCCTGGGGCTACTACA 179
|||| |||||||||||||||||||||||| || |||||||||||||| |||||||||||
6831R-2.in silico 121 TGAG-CAGAACCAGCAGGGGGTTTGGTTGGAA-CCGGGACGCACCCT-GGGCTACTACA 179
6831R-2.IR_full 181 TACTGCACAACCCAGGATACGCTGGAGTATCGCCGCAAAGACCACNNAAACCCTACGTGT 239
|||||||||| ||||||||||||||||||||||||| |||| ||| ||||||||||||
6831R-2.in silico 181 TACTGCACAA-CCAGGATACGCTGGAGTATCGCCGC-AAGA-CAC--GAACCCTACGTGT 239
6831R-2.IR_full 241 TCNCATGTTGGATGGCGCTGTAAAGACCATTCTGGTGGATGACTNCCCAGCCGGTGTCGC 299
|| |||||||||||||||||||||||||||||||||||||||| |||||||||||||||
6831R-2.in silico 241 TCGTATGTTGGATGGCGCTGTAAAGACCATTCTGGTGGATGACT-CCCAGCCGGTGTCGC 299
6831R-2.IR_full 301 AGCTTATGGTGGTCATCTGCACAAAGATCGGCATCACCAATCATGAGGAATATGGATTGG 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6831R-2.in silico 301 AGCTTATGGTGGTCATCTGCACAAAGATCGGCATCACCAATCATGAGGAATATGGATTGG 359
6831R-2.IR_full 361 TGCGCGAGGACAATGAGGCGCAGAATGAGAATCTGCCGGACAACAAGTTTGGAACGCTTA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6831R-2.in silico 361 TGCGCGAGGACAATGAGGCGCAGAATGAGAATCTGCCGGACAACAAGTTTGGAACGCTTA 419
6831R-2.IR_full 421 CCCTTAAGCGTAAGATTATGGAGAAGGATCGCGATGCCAAAATGGAGAGTTTGCGCAAGA 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6831R-2.in silico 421 CCCTTAAGCGTAAGATTATGGAGAAGGATCGCGATGCCAAAATGGAGAGTTTGCGCAAGA 479
6831R-2.IR_full 481 AACTAAAGACGGACGATGAAATGAACTGGGTGGATGTG 517
||||||||||||||||||||||||||||||||||||||
6831R-2.in silico 481 AACTAAAGACGGACGATGAAATGAACTGGGTGGATGTG 517
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_139981.2 |
CG6831-RA (rhea), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165878.1 |
CG30046-RB (CG30046), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_078693.2 |
CG9565-RA (Nep3), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_141248.2 |
CG31536-RC, transcript variant C (Cdep), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001043211.1 |
CG31536-RE, transcript variant E (Cdep), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_169022.1 |
CG31536-RA, transcript variant A (Cdep), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_139563.2 |
CG10359-RA (CG10359), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_142753.2 |
CG5740-RB, transcript variant B (CG5740), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_169996.1 |
CG5740-RA, transcript variant A (CG5740), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_135778.1 |
CG16815-RA (CG16815), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_079119.2 |
CG4354-RA (slbo), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135265.1 |
CG4971-RA (Wnt10), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135338.1 |
CG8673-RA (CG8673), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_168357.2 |
CG32045-RB, transcript variant B (fry), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001043133.1 |
CG32045-RD, transcript variant D (fry), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_133073.2 |
CG6394-RA, transcript variant A (GalNAc-T2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167623.1 |
CG6394-RB, transcript variant B (GalNAc-T2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164843.1 |
CG9556-RA, transcript variant A (alien), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_078793.4 |
CG9556-RB, transcript variant B (alien), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_080013.2 |
CG6050-RA (EfTuM), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_057685.3 |
CG11527-RA (Tig), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_057984.3 |
CG3460-RA (Nmd3), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_135281.1 |
CG6630-RA (CG6630), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_079913.2 |
CG8110-RA, transcript variant A (syd), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_168245.2 |
CG8110-RB, transcript variant B (syd), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_142803.1 |
CG5326-RA, transcript variant A (CG5326), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_132681.1 |
CG15760-RA (CG15760), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_137794.2 |
CG11073-RA (CG11073), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_079455.3 |
CG12306-RA, transcript variant A (polo), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_143439.2 |
CG2010-RB, transcript variant B (CG2010), mRNA |
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