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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6819R-3 |
| Symbol |
mbo |
Full Name |
members only |
| CG No |
CG6819 |
Old CG No |
CG6819 |
| Synonyms |
Dnup 88, dNup88, Dnup88, DNup88, Nup88, l(3)05043, l(3)S146507, unnamed, 1465/7, 1465/07, CG6819, anon-WO0118547.263, mbo, Mbo |
| Accession No (Link to NCBI) |
NM_079606.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTGGTGCAAAGGACTCAGAAC |
| Primer Seq. 3' |
aatctagaggtaccTTATTGGCCCCACTTCGAAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GTGGTGCAAA GGACTCAGAA CCTGCTGGAC TGCAAGGACG ATCTGCTCTT TGCCTGGCAC
0061 GCGAAGGACA GCTGTCTGTT GGTTCGCAAC TGNGCGCTCA TCGCTGGCGG CAAAGGTGAA
0121 TATCCAGTTC CAGACACTGA TTCCATCGAG CTTGGTGAGC CTGGAGGTGG ACCGCGTGCT
0181 GGCCTCCAAC GAGGGCTCCC TCGTGGCACT AAGTGGACCG CGCGGCGTTG TCATAATGGA
0241 GCTGCCCCGC CGCTGGGGCC CCGATGGATA CTACAAGGAT GGCAAGCCAG TGATCACCTG
0301 CCGCACGTTC GGCCTGGACA CTCAGCTTTT CCTAAAAAAC CCGCACTTGG AAGTGCGCCA
0361 GGTCCGCTGG CATCCGCACT CCGTGTCAGA CTCCACGCTG CTCGTGCTGC TCAACAACAA
0421 CACGATTCGA GTGTACAATC ACTCCAAGTT GCGCCACGTG TGGCAGGTGG GTCCCCCGGT
0481 GCTTCGAAGT GGGGCCAATA A |
| in silico PCR Fragment |
0001 GTGGTGCAAA GGACTCAGAA CCTGCTGGAC TGCAAGGACG ATCTGCTCTT TGCCTGGCAC
0061 GCGAAGGACA GCTGTCTGTT GGTTCGCAAC TG-GCGCTCA TCGCTGGCGG CAAAGGTGAA
0121 TATCCAGTTC CAGACACTGA TTCCATCGAG CTTGGTGAGC CTGGAGGTGG ACCGCGTGCT
0181 GGCCTCCAAC GAGGGCTCCC TCGTGGCACT AAGTGGACCG CGCGGCGTTG TCATAATGGA
0241 GCTGCCCCGC CGCTGGGGCC CCGATGGATA CTACAAGGAT GGCAAGCCAG TGATCACCTG
0301 CCGCACGTTC GGCCTGGACA CTCAGCTTTT CCTAAAAAAC CCGCACTTGG AAGTGCGCCA
0361 GGTCCGCTGG CATCCGCACT CCGTGTCAGA CTCCACGCTG CTCGTGCTGC TCAACAACAA
0421 CACGATTCGA GTGTACAATC ACTCCAAGTT GCGCCACGTG TGGCAGGTGG GTCCCCCGGT
0481 GCTTCGAAGT GGGGCCAATA A |
| Assemble Data |
6819R-3.IR_full 1 GTGGTGCAAAGGACTCAGAACCTGCTGGACTGCAAGGACGATCTGCTCTTTGCCTGGCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 1 GTGGTGCAAAGGACTCAGAACCTGCTGGACTGCAAGGACGATCTGCTCTTTGCCTGGCAC 60
6819R-3.IR_full 61 GCGAAGGACAGCTGTCTGTTGGTTCGCAACTGNGCGCTCATCGCTGGCGGCAAAGGTGAA 120
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
6819R-3.in silico 61 GCGAAGGACAGCTGTCTGTTGGTTCGCAACTG-GCGCTCATCGCTGGCGGCAAAGGTGAA 120
6819R-3.IR_full 121 TATCCAGTTCCAGACACTGATTCCATCGAGCTTGGTGAGCCTGGAGGTGGACCGCGTGCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 121 TATCCAGTTCCAGACACTGATTCCATCGAGCTTGGTGAGCCTGGAGGTGGACCGCGTGCT 180
6819R-3.IR_full 181 GGCCTCCAACGAGGGCTCCCTCGTGGCACTAAGTGGACCGCGCGGCGTTGTCATAATGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 181 GGCCTCCAACGAGGGCTCCCTCGTGGCACTAAGTGGACCGCGCGGCGTTGTCATAATGGA 240
6819R-3.IR_full 241 GCTGCCCCGCCGCTGGGGCCCCGATGGATACTACAAGGATGGCAAGCCAGTGATCACCTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 241 GCTGCCCCGCCGCTGGGGCCCCGATGGATACTACAAGGATGGCAAGCCAGTGATCACCTG 300
6819R-3.IR_full 301 CCGCACGTTCGGCCTGGACACTCAGCTTTTCCTAAAAAACCCGCACTTGGAAGTGCGCCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 301 CCGCACGTTCGGCCTGGACACTCAGCTTTTCCTAAAAAACCCGCACTTGGAAGTGCGCCA 360
6819R-3.IR_full 361 GGTCCGCTGGCATCCGCACTCCGTGTCAGACTCCACGCTGCTCGTGCTGCTCAACAACAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 361 GGTCCGCTGGCATCCGCACTCCGTGTCAGACTCCACGCTGCTCGTGCTGCTCAACAACAA 420
6819R-3.IR_full 421 CACGATTCGAGTGTACAATCACTCCAAGTTGCGCCACGTGTGGCAGGTGGGTCCCCCGGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6819R-3.in silico 421 CACGATTCGAGTGTACAATCACTCCAAGTTGCGCCACGTGTGGCAGGTGGGTCCCCCGGT 480
6819R-3.IR_full 481 GCTTCGAAGTGGGGCCAATAA 501
|||||||||||||||||||||
6819R-3.in silico 481 GCTTCGAAGTGGGGCCAATAA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079606.2 |
CG6819-RA (mbo), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_057346.3 |
CG3258-RA (ase), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_133134.2 |
CG12359-RA (Ulp1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_139737.1 |
CG13287-RA (CG13287), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_164444.1 |
CG31663-RA (CG31663), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079490.2 |
CG5837-RA (Hem), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141639.1 |
CG16779-RA (CG16779), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_130653.2 |
CG13759-RA (CG13759), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136777.2 |
CG30015-RB, transcript variant B (CG30015), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165802.1 |
CG30015-RA, transcript variant A (CG30015), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_166535.1 |
CG4444-RA (px), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_142917.2 |
CG10300-RA (CG10300), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_170004.1 |
CG7070-RB, transcript variant B (PyK), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_079724.2 |
CG7070-RA, transcript variant A (PyK), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_143462.2 |
CG1969-RA, transcript variant A (CG1969), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_170422.1 |
CG1969-RB, transcript variant B (CG1969), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_134540.1 |
CG15462-RA (CG15462), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_057233.3 |
CG10160-RA (ImpL3), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_142435.2 |
CG7183-RA (CG7183), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_130598.2 |
CG14805-RA (CG14805), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_001043084.1 |
CG34123-RD, transcript variant D (CG34123), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176468.1 |
CG3359-RD, transcript variant D (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176475.1 |
CG3359-RF, transcript variant F (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176469.1 |
CG3359-RL, transcript variant L (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176472.1 |
CG3359-RK, transcript variant K (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176470.1 |
CG3359-RP, transcript variant P (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176466.1 |
CG3359-RH, transcript variant H (mfas), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176477.1 |
CG3359-RM, transcript variant M (mfas), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
- |
|
|
|
|
