NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6767R-2

Symbol CG6767 Full Name CG6767

CG No CG6767 Old CG No CG6767

Synonyms CG6767

Accession No (Link to NCBI) NM_140088.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGATCTCGGCAAGGTGGTTA

Primer Seq. 3'

aatctagaggtaccCAGAGCGAACTCCACGTTCAG

Predicted Fragment Size

IR fragment full Seq

0001 TCGATCTCGG CAAGGTGGTT ACGAAGAAGT TCAGCAACTT GGAGACCTGT GTGGAAATTG 
0061 GGGAATCGGT GCGCGGCGAG GATGTGTACA TCGTGCAATC GGGATCCGGC GAGATCAACG 
0121 ATAACTTGAT GGAGCTGCTG ATCATGATCA ATGCGTGCAA AATTGCCTCA GCCTCTCGTG 
0181 TTACAGCTGT GATTCCTTGC TTTCCATATG CCCGCCAAGA TAAAAAAGAC AAGAGTCGTG 
0241 CGCCCATCTC GGCCAAATTG GTGGCCAACA TGCTGTCCGT TGCTGGAGCG GATCACATCA 
0301 TCACCATGGA TCTGCACGCC TCACAGATTC AGGGCTTCTT TGATATACCA GTTGATAATC 
0361 TCTNATGcCG AGCCGGCGGT ACTCAAGTGG ATCAAGGAGA ACATTCCCGA GTGGAAGAAC 
0421 TCGATCATTG TGTCGCCCGA TGCTgGCGGT GCCAAGCGTG TCACCTCAAT TGCCGATCGA 
0481 CTGAACGTGG AGTTCGCTCT G

in silico PCR Fragment

0001 TCGATCTCGG CAAGGTGGTT ACGAAGAAGT TCAGCAACTT GGAGACCTGT GTGGAAATTG 
0061 GGGAATCGGT GCGCGGCGAG GATGTGTACA TCGTGCAATC GGGATCCGGC GAGATCAACG 
0121 ATAACTTGAT GGAGCTGCTG ATCATGATCA ATGCGTGCAA AATTGCCTCA GCCTCTCGTG 
0181 TTACAGCTGT GATTCCTTGC TTTCCATATG CCCGCCAAGA TAAAAAAGAC AAGAGTCGTG 
0241 CGCCCATCTC GGCCAAATTG GTGGCCAACA TGCTGTCCGT TGCTGGAGCG GATCACATCA 
0301 TCACCATGGA TCTGCACGCC TCACAGATTC AGGGCTTCTT TGATATACCA GTTGATAATC 
0361 TCT-ATGCCG AGCCGGCGGT ACTCAAGTGG ATCAAGGAGA ACATTCCCGA GTGGAAGAAC 
0421 TCGATCATTG TGTCGCCCGA TGCTGGCGGT GCCAAGCGTG TCACCTCAAT TGCCGATCGA 
0481 CTGAACGTGG AGTTCGCTCT G

Assemble Data


6767R-2.IR_full       1   TCGATCTCGGCAAGGTGGTTACGAAGAAGTTCAGCAACTTGGAGACCTGTGTGGAAATTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     1   TCGATCTCGGCAAGGTGGTTACGAAGAAGTTCAGCAACTTGGAGACCTGTGTGGAAATTG 60


6767R-2.IR_full       61  GGGAATCGGTGCGCGGCGAGGATGTGTACATCGTGCAATCGGGATCCGGCGAGATCAACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     61  GGGAATCGGTGCGCGGCGAGGATGTGTACATCGTGCAATCGGGATCCGGCGAGATCAACG 120


6767R-2.IR_full       121 ATAACTTGATGGAGCTGCTGATCATGATCAATGCGTGCAAAATTGCCTCAGCCTCTCGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     121 ATAACTTGATGGAGCTGCTGATCATGATCAATGCGTGCAAAATTGCCTCAGCCTCTCGTG 180


6767R-2.IR_full       181 TTACAGCTGTGATTCCTTGCTTTCCATATGCCCGCCAAGATAAAAAAGACAAGAGTCGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     181 TTACAGCTGTGATTCCTTGCTTTCCATATGCCCGCCAAGATAAAAAAGACAAGAGTCGTG 240


6767R-2.IR_full       241 CGCCCATCTCGGCCAAATTGGTGGCCAACATGCTGTCCGTTGCTGGAGCGGATCACATCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     241 CGCCCATCTCGGCCAAATTGGTGGCCAACATGCTGTCCGTTGCTGGAGCGGATCACATCA 300


6767R-2.IR_full       301 TCACCATGGATCTGCACGCCTCACAGATTCAGGGCTTCTTTGATATACCAGTTGATAATC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     301 TCACCATGGATCTGCACGCCTCACAGATTCAGGGCTTCTTTGATATACCAGTTGATAATC 360


6767R-2.IR_full       361 TCTNATGCCGAGCCGGCGGTACTCAAGTGGATCAAGGAGAACATTCCCGAGTGGAAGAAC 420
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     361 TCT-ATGCCGAGCCGGCGGTACTCAAGTGGATCAAGGAGAACATTCCCGAGTGGAAGAAC 420


6767R-2.IR_full       421 TCGATCATTGTGTCGCCCGATGCTGGCGGTGCCAAGCGTGTCACCTCAATTGCCGATCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6767R-2.in silico     421 TCGATCATTGTGTCGCCCGATGCTGGCGGTGCCAAGCGTGTCACCTCAATTGCCGATCGA 480


6767R-2.IR_full       481 CTGAACGTGGAGTTCGCTCTG 501
                          |||||||||||||||||||||
6767R-2.in silico     481 CTGAACGTGGAGTTCGCTCTG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	551	0	0	0	NM_168360.1	CG6767-RB, transcript variant B (CG6767), mRNA
84.39	465	3	2	2	NM_140088.2	CG6767-RA, transcript variant A (CG6767), mRNA
0	0	8	9	10	NM_170510.1	CG2246-RA, transcript variant A (CG2246), mRNA
0	0	8	9	10	NM_170511.1	CG2246-RC, transcript variant C (CG2246), mRNA
0	0	8	9	10	NM_170507.1	CG2246-RB, transcript variant B (CG2246), mRNA
0	0	8	9	10	NM_170508.1	CG2246-RD, transcript variant D (CG2246), mRNA
0	0	8	9	10	NM_170509.1	CG2246-RF, transcript variant F (CG2246), mRNA
0	0	8	9	10	NM_143556.2	CG2246-RE, transcript variant E (CG2246), mRNA
0	0	4	2	5	NM_135962.1	CG17905-RA (CG17905), mRNA
0	0	2	2	4	NM_132185.1	CG15325-RA (CG15325), mRNA
0	0	1	9	3	NM_078745.2	CG12676-RA (ed), mRNA
0	0	1	3	4	NM_080148.2	CG2522-RA (Gtp-bp), mRNA
0	0	1	3	3	NM_135386.3	CG13401-RA, transcript variant A (U26), mRNA
0	0	1	3	3	NM_205938.1	CG13401-RB, transcript variant B (U26), mRNA
0	0	1	2	4	NM_132206.1	CG2253-RA (Upf2), mRNA
0	0	1	2	3	NM_079545.2	CG1031-RA (alpha-Est1), mRNA
0	0	1	2	2	NM_132917.1	CG13010-RA (CG13010), mRNA
0	0	1	2	2	NM_164371.1	CG11455-RA, transcript variant A (CG11455), mRNA
0	0	1	2	2	NM_164372.1	CG11455-RB, transcript variant B (CG11455), mRNA
0	0	1	2	2	NM_134664.1	CG17075-RA (CG17075), mRNA
0	0	1	1	6	NM_132225.1	CG18262-RA (CG18262), mRNA
0	0	0	6	6	NM_132056.3	CG4790-RA (fs(1)M3), mRNA
0	0	0	5	19	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	5	6	NM_136595.1	CG13744-RA (CG13744), mRNA
0	0	0	5	2	NM_132151.1	CG14427-RA (CG14427), mRNA
0	0	0	4	9	NM_142312.1	CG14905-RA (CG14905), mRNA
0	0	0	4	6	NM_078535.2	CG1634-RA, transcript variant A (Nrg), mRNA
0	0	0	4	6	NM_167160.1	CG1634-RB, transcript variant B (Nrg), mRNA
0	0	0	4	6	NM_206657.1	CG1634-RC, transcript variant C (Nrg), mRNA
0	0	0	4	5	NM_078772.2	CG13772-RA (neuroligin), mRNA

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